2004
MMP‐2 null mice exhibit an early onset and severe experimental autoimmune encephalomyelitis due to an increase in MMP‐9 expression and activity
Esparza J, Kruse M, Lee J, Michaud M, Madri JA. MMP‐2 null mice exhibit an early onset and severe experimental autoimmune encephalomyelitis due to an increase in MMP‐9 expression and activity. The FASEB Journal 2004, 18: 1682-1691. PMID: 15522913, DOI: 10.1096/fj.04-2445com.Peer-Reviewed Original ResearchMeSH KeywordsAge of OnsetAnimalsBasement MembraneBlood-Brain BarrierBrainCD3 ComplexCell MovementEncephalomyelitis, Autoimmune, ExperimentalEnzyme ActivationGene Expression Regulation, EnzymologicMatrix Metalloproteinase 14Matrix Metalloproteinase 2Matrix Metalloproteinase 9Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMice, KnockoutT-Lymphocyte SubsetsT-LymphocytesConceptsMMP-2 KO miceExperimental autoimmune encephalomyelitisMMP-9 expressionAutoimmune encephalomyelitisEarly onsetWT miceKO miceMMP-9Bone marrowSevere experimental autoimmune encephalomyelitisMMP-2 null miceSimilar early onsetWT bone marrowClinical disease scoresMMP-2-deficient miceKO bone marrowMatrix metalloproteinase-2MT1-MMP expressionEndothelial cell monolayersSevere diseaseDisease scoreMetalloproteinase-2Collagen type IVMMP-2Matrix metalloproteinase
2002
Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpression
2000
Matrix metalloproteinase activity is required for activity-induced angiogenesis in rat skeletal muscle
Haas T, Milkiewicz M, Davis S, Zhou A, Egginton S, Brown M, Madri J, Hudlicka O. Matrix metalloproteinase activity is required for activity-induced angiogenesis in rat skeletal muscle. AJP Heart And Circulatory Physiology 2000, 279: h1540-h1547. PMID: 11009439, DOI: 10.1152/ajpheart.2000.279.4.h1540.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCapillariesCell DivisionDipeptidesElectric StimulationImmunohistochemistryMatrix Metalloproteinase 2Matrix Metalloproteinase InhibitorsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMicroscopy, ElectronMotor ActivityMuscle, SkeletalNeovascularization, PhysiologicProtease InhibitorsRatsRNA, MessengerConceptsMembrane proteinsBasement membrane proteinsEndothelial cell sprout formationRat skeletal muscleSkeletal muscleMatrix metalloproteinasesMembrane type 1Inflammation-mediated angiogenesisPhysiological angiogenesisBasement membraneCell proliferationMMP proteolysisProtein levelsProteolysisSprout formationMajor classesCritical roleProteinMatrix metalloproteinase activityMetalloproteinase activityProliferationAngiogenesisNew capillariesMembraneMMP inhibition
1999
Egr-1 Mediates Extracellular Matrix-driven Transcription of Membrane Type 1 Matrix Metalloproteinase in Endothelium*
Haas T, Stitelman D, Davis S, Apte S, Madri J. Egr-1 Mediates Extracellular Matrix-driven Transcription of Membrane Type 1 Matrix Metalloproteinase in Endothelium*. Journal Of Biological Chemistry 1999, 274: 22679-22685. PMID: 10428849, DOI: 10.1074/jbc.274.32.22679.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCloning, MolecularDNA-Binding ProteinsEarly Growth Response Protein 1Endothelium, VascularExtracellular MatrixGene Expression Regulation, EnzymologicHalf-LifeImmediate-Early ProteinsMatrix Metalloproteinase 14Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMolecular Sequence DataNeovascularization, PhysiologicProtein BindingRatsRNA, MessengerSp1 Transcription FactorTranscription FactorsTranscription, GeneticUp-RegulationConceptsMembrane type 1 matrix metalloproteinaseEgr-1MT1-MMPTranscription factor Egr-1Number of proteinsExtracellular matrix environmentEnhanced transcriptional activityEndothelial cellsTranscriptional activityPromoter correlatesIncreased transcriptionCellular invasionInvasive phenotypeMatrix metalloproteinaseTranscriptionMatrix environmentMatrix metalloproteinase activityMetalloproteinase activityCellsMatrix metalloproteinasesInvasionIncrease productionAngiogenesisMetalloproteinaseProteinExtracellular Matrix-Driven Matrix Metalloproteinase Production in Endothelial Cells Implications for Angiogenesis
Haas T, Madri J. Extracellular Matrix-Driven Matrix Metalloproteinase Production in Endothelial Cells Implications for Angiogenesis. Trends In Cardiovascular Medicine 1999, 9: 70-77. PMID: 10578520, DOI: 10.1016/s1050-1738(99)00014-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CommunicationCells, CulturedEndothelium, VascularExtracellular MatrixHumansMatrix Metalloproteinase 2Matrix MetalloproteinasesMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PhysiologicConceptsInterstitial matrix moleculesCell-extracellular matrix interactionsBasement membrane matrixMatrix metalloproteinasesTranscriptional activationBlood vessel growthMatrix interactionsNew blood vessel growthMatrix moleculesCell surfaceExtracellular matrixMT1-MMPAdjacent cellsCell interactionsMatrix metalloproteinase productionCurrent understandingVessel growthParticular matrix metalloproteinasesEndothelial cell interactionsPotential roleAngiogenesisMetalloproteinase productionBasement membraneMMP-2Signaling
1998
The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis.
Graesser D, Mahooti S, Haas T, Davis S, Clark R, Madri J. The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis. Laboratory Investigation 1998, 78: 1445-58. PMID: 9840619.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDCell AdhesionCell MembraneCell MovementCells, CulturedClone CellsEncephalomyelitis, Autoimmune, ExperimentalEndothelium, VascularGelatinasesIntegrin alpha4Matrix Metalloproteinase 14Matrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMice, Inbred StrainsProtease InhibitorsT-LymphocytesTissue Inhibitor of Metalloproteinase-2TransfectionConceptsExperimental autoimmune encephalomyelitisT cell clonesRecombinant vascular cell adhesion molecule-1Autoreactive T cell clonesAlpha4 integrinsMMP-2EAE inductionAutoimmune encephalomyelitisMyelin basic protein-reactive T cell clonesDisease processMatrix metalloproteinaseVascular cell adhesion molecule-1Cell adhesion molecule-1Human multiple sclerosisSusceptible mouse strainsAdhesion molecule-1T cell transmigrationMatrix metalloproteinase-2Microvascular endothelial cellsAlpha4 integrin expressionExpression of alpha4Adoptive transferMultiple sclerosisMouse modelEndothelial cell layerThree-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*
Haas T, Davis S, Madri J. Three-dimensional Type I Collagen Lattices Induce Coordinate Expression of Matrix Metalloproteinases MT1-MMP and MMP-2 in Microvascular Endothelial Cells*. Journal Of Biological Chemistry 1998, 273: 3604-3610. PMID: 9452488, DOI: 10.1074/jbc.273.6.3604.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCollagenDrug CombinationsEndothelium, VascularEnzyme ActivationEnzyme InhibitorsGelatinasesLamininMatrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNeovascularization, PhysiologicProtein ConformationProteoglycansRatsRats, Sprague-DawleyRNA, MessengerSignal TransductionTissue Inhibitor of Metalloproteinase-2ConceptsMT1-MMPTwo-dimensional cultureEndothelial cellsThree-dimensional collagen matrixEndothelial cell organizationEndothelial cell angiogenesisEndothelial cell migrationCell organizationCoordinate expressionPutative activatorMT1-MMP activationMMP-2Matrix metalloproteinasesCell migrationFunctional roleMatrix interactionsMatrix remodelingCollagen matrixCell angiogenesisEndothelial cell networksMMP-2 proteinProtein