2023
CD31 as a probable responding and gate-keeping protein of the blood-brain barrier and the risk of Alzheimer’s disease
Zhang Z, Gan Q, Han J, Tao Q, Qiu W, Madri J. CD31 as a probable responding and gate-keeping protein of the blood-brain barrier and the risk of Alzheimer’s disease. Cerebrovascular And Brain Metabolism Reviews 2023, 43: 1027-1041. PMID: 37051650, PMCID: PMC10291450, DOI: 10.1177/0271678x231170041.Peer-Reviewed Original ResearchConceptsPlatelet endothelial cell adhesion moleculeImmune cellsDisease riskBlood-brain barrier permeabilityMajor genetic risk factorBlood-brain barrierNeuronal cell injuryEndothelial cell adhesion moleculesAlzheimer's disease riskGenetic risk factorsPeripheral inflammationBrain axisAPOE4 carriersAD pathogenesisRisk factorsBarrier permeabilityAD developmentCell adhesion moleculeCell injuryImmune systemAlzheimer's diseaseCD31Transendothelial migrationPotential drug targetsAdhesion molecules
2020
Glycocalyx‐Like Hydrogel Coatings for Small Diameter Vascular Grafts
Dimitrievska S, Wang J, Lin T, Weyers A, Bai H, Qin L, Li G, Cai C, Kypson A, Kristofik N, Gard A, Sundaram S, Yamamoto K, Wu W, Zhao L, Kural M, Yuan Y, Madri J, Kyriakides T, Linhardt R, Niklason L. Glycocalyx‐Like Hydrogel Coatings for Small Diameter Vascular Grafts. Advanced Functional Materials 2020, 30 DOI: 10.1002/adfm.201908963.Peer-Reviewed Original ResearchA Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells.
Boisvert EM, Means RE, Michaud M, Thomson JJ, Madri JA, Katz SG. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 PMID: 32202516, PMCID: PMC7245934, DOI: 10.3791/60379.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsCell typesStem cellsIntrinsic developmental cuesHuman embryonic stem cellsHuman pluripotent stem cellsBrain organoidsBrain cell typesPluripotent stem cellsBasement membrane matrixMultiple cell typesDevelopmental cuesUse of organoidsExogenous growth factorsQuantitative reverse transcription polymerase chain reactionMultitude of diseasesHuman brain organoidsOrganoid growthSingle cellsReal-time quantitative reverse transcription polymerase chain reactionSpatial organizationOrganoidsGenetic disordersGrowth factorReverse transcription-polymerase chain reactionA Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
Boisvert E, Means R, Michaud M, Thomson J, Madri J, Katz S. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal Of Visualized Experiments 2020 DOI: 10.3791/60379-v.Peer-Reviewed Case Reports and Technical Notes
2019
Somatic PRKAR1A mutation in sporadic atrial myxoma with cerebral parenchymal metastases: a case report
Roque A, Kimbrough T, Traner C, Baehring JM, Huttner A, Adams J, Canosa S, Sklar J, Madri JA. Somatic PRKAR1A mutation in sporadic atrial myxoma with cerebral parenchymal metastases: a case report. Journal Of Medical Case Reports 2019, 13: 389. PMID: 31874650, PMCID: PMC6930684, DOI: 10.1186/s13256-019-2317-z.Peer-Reviewed Original ResearchMeSH KeywordsBrain NeoplasmsCarney ComplexChemoradiotherapyCyclic AMP-Dependent Protein Kinase RIalpha SubunitDopamine AgentsExome SequencingFemaleGene Expression Regulation, NeoplasticGenes, Tumor SuppressorGerm-Line MutationHeart NeoplasmsHumansIntracranial HemorrhagesMemantineMiddle AgedMyxomaTreatment OutcomeConceptsAtrial myxomaSporadic tumorsExtra-cardiac complicationsMetastatic cardiac myxomaMajority of tumorsDissemination of tumorsIntracranial hemorrhagic lesionsWhole-exome sequencingAutosomal dominant conditionConclusionsOur patientsSporadic myxomasInvasive tumor cellsParenchymal metastasesCardiac myxomaCase reportClinical behaviorHemorrhagic lesionsAneurysm formationBenign neoplasmsMyxomaSporadic lesionsVascular wallCarney complexTumorsGermline mutationsMinocycline mitigates the effect of neonatal hypoxic insult on human brain organoids
Boisvert EM, Means RE, Michaud M, Madri JA, Katz SG. Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids. Cell Death & Disease 2019, 10: 325. PMID: 30975982, PMCID: PMC6459920, DOI: 10.1038/s41419-019-1553-x.Peer-Reviewed Original ResearchConceptsNeonatal hypoxic injuryBrain developmentEfficacy of minocyclineLow birth weightUse of minocyclineEffects of hypoxiaNormal brain developmentCerebral organoid modelHuman brain organoidsLater time pointsAnimal model systemsNeonatal hypoxicDevastating causeCerebral palsySignificant morbidityHuman brain developmentNeurological consequencesBirth weightHypoxic injuryNeuronal deathCortical neuronsInjury resultsGlial cellsForebrain markersPotential treatment
2018
Immunocytochemical Techniques in Connective Tissue Research
Roll F, Madri J. Immunocytochemical Techniques in Connective Tissue Research. 2018, 49-88. DOI: 10.1201/9781351073424-3.Peer-Reviewed Original ResearchThe Immunobiology and Immunogenetics of the Collagens*
Kemp J, Madri J. The Immunobiology and Immunogenetics of the Collagens*. 2018, 175-186. DOI: 10.1201/9781351073424-7.Peer-Reviewed Original Research
2017
MMP-2: A modulator of neuronal precursor activity and cognitive and motor behaviors
Li Q, Michaud M, Shankar R, Canosa S, Schwartz M, Madri JA. MMP-2: A modulator of neuronal precursor activity and cognitive and motor behaviors. Behavioural Brain Research 2017, 333: 74-82. PMID: 28666838, DOI: 10.1016/j.bbr.2017.06.041.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell MovementCell ProliferationCells, CulturedCognitionExploratory BehaviorGene Expression RegulationMatrix Metalloproteinase 2MiceMice, Inbred C57BLMice, KnockoutMotor ActivityNerve Tissue ProteinsNeural Stem CellsNeurogenesisOncogene Protein v-aktProliferating Cell Nuclear AntigenReceptors, CXCR4Spatial LearningConceptsNeural precursor cellsBroad substrate specificityNeurosphere formationAdherent neurospheresSecondary neurosphere formationNPC activitySubstrate specificityNPC numberCell surface moleculesZinc-containing enzymesAkt activationAbsence of MMP2Cell typesExtracellular matrixActivity assaysPrecursor cellsImportant roleNPC migrationMatrix metalloproteinase2Surface moleculesExpressionKO miceBioactive moleculesNestin expressionMMP2CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation
Kuwahara G, Hashimoto T, Tsuneki M, Yamamoto K, Assi R, Foster TR, Hanisch JJ, Bai H, Hu H, Protack CD, Hall MR, Schardt JS, Jay SM, Madri JA, Kodama S, Dardik A. CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation. Arteriosclerosis Thrombosis And Vascular Biology 2017, 37: 1147-1156. PMID: 28450292, PMCID: PMC5467640, DOI: 10.1161/atvbaha.117.309385.Peer-Reviewed Original ResearchConceptsExtracellular matrix depositionKnockout miceExtracellular matrix componentsExtracellular matrix productionMatrix depositionAdhesion molecule-1 expressionM2 macrophagesProtein 1Matrix productionCell adhesion molecule-1 expressionMolecule-1 expressionProtein expressionMatrix componentsCD44 knockout miceProtein-1 expressionMajor receptorCD44 activityMaturationVascular cell adhesion molecule-1 expressionAdhesion moleculesExpressionCD44 mRNAChemoattractant protein-1 expressionWild-type C57BL/6JArteriovenous fistulaNOD Mice Having a Lyn Tyrosine Kinase Mutation Exhibit Abnormal Neutrophil Chemotaxis
Wu Y, Hannigan M, Zhan L, Madri JA, Huang C. NOD Mice Having a Lyn Tyrosine Kinase Mutation Exhibit Abnormal Neutrophil Chemotaxis. Journal Of Cellular Physiology 2017, 232: 1689-1695. PMID: 27591397, DOI: 10.1002/jcp.25583.Peer-Reviewed Original ResearchAs human lung microvascular endothelia achieve confluence, src family kinases are activated, and tyrosine-phosphorylated p120 catenin physically couples NEU1 sialidase to CD31
Hyun SW, Liu A, Liu Z, Lillehoj EP, Madri JA, Reynolds AB, Goldblum SE. As human lung microvascular endothelia achieve confluence, src family kinases are activated, and tyrosine-phosphorylated p120 catenin physically couples NEU1 sialidase to CD31. Cellular Signalling 2017, 35: 1-15. PMID: 28343945, DOI: 10.1016/j.cellsig.2017.03.014.Peer-Reviewed Original ResearchMeSH KeywordsCateninsCell LineCell-Free SystemDelta CateninEndothelial CellsHumansLungMicrovesselsN-Acetylneuraminic AcidNeovascularization, PhysiologicNeuraminidasePhosphorylationPlatelet Endothelial Cell Adhesion Molecule-1Protein BindingProtein Interaction MapsProto-Oncogene Proteins c-fynProto-Oncogene Proteins c-yesSignal TransductionSrc-Family KinasesIncreased Oxidative Stress and Hypoxia Inducible Factor-1 Expression during Arteriovenous Fistula Maturation
Sadaghianloo N, Yamamoto K, Bai H, Tsuneki M, Protack CD, Hall MR, Declemy S, Hassen-Khodja R, Madri J, Dardik A. Increased Oxidative Stress and Hypoxia Inducible Factor-1 Expression during Arteriovenous Fistula Maturation. Annals Of Vascular Surgery 2017, 41: 225-234. PMID: 28163173, PMCID: PMC5411319, DOI: 10.1016/j.avsg.2016.09.014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaArteriovenous Shunt, SurgicalGene Expression RegulationHeme Oxygenase-1Hydrogen PeroxideHyperplasiaHypoxia-Inducible Factor 1, alpha SubunitMaleMembrane ProteinsMice, Inbred C57BLNADPH Oxidase 2NeointimaOxidative StressReactive Oxygen SpeciesSignal TransductionTime FactorsTyrosineVascular Endothelial Growth Factor AVascular PatencyVena Cava, InferiorConceptsHeme oxygenase-1Arteriovenous fistulaAVF maturationNOX-2HIF-1αOxidative stressHypoxia-inducible factor 1 (HIF-1) expressionSham-operated micePoor clinical resultsHIF-1α immunoreactivityInferior vena cavaArteriovenous fistula maturationVascular endothelial growth factorHypoxia-inducible factor-1 (HIF-1) pathwayFactor-1 expressionEndothelial growth factorHIF-1 pathwayHuman AVF maturationQuantitative polymerase chain reactionOxidative stress increasesAortocaval fistulaFistula maturationVena cavaClinical resultsPolymerase chain reactionThe role of endothelial HIF-1 αin the response to sublethal hypoxia in C57BL/6 mouse pups
Li Q, Michaud M, Park C, Huang Y, Couture R, Girodano F, Schwartz ML, Madri JA. The role of endothelial HIF-1 αin the response to sublethal hypoxia in C57BL/6 mouse pups. Laboratory Investigation 2017, 97: 356-369. PMID: 28092362, DOI: 10.1038/labinvest.2016.154.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornApoptosisBlotting, WesternCell HypoxiaCell ProliferationCells, CulturedDentate GyrusEndothelial CellsFemaleHypoxiaHypoxia-Inducible Factor 1, alpha SubunitLateral VentriclesMaleMice, Inbred C57BLMice, KnockoutMice, TransgenicMicroscopy, FluorescenceMotor ActivityNeural Stem CellsConceptsHIF-1 αBrain microvascular endothelial cellsNeuronal precursor cellsSubventricular zoneMicrovascular endothelial cellsOpen-field activityEndothelial cellsSublethal hypoxiaHIF-1 α expressionOpen-field activity testChronic sublethal hypoxiaEndothelial HIF-1Hypoxic conditionsC57BL/6 mouse pupsGender-specific differencesPremature birthC57BL/6 WTDentate gyrusHippocampal tissueDeficient miceΑ expressionMouse pupsMotor handicapParacrine effectsDentate gyrus tissue
2016
Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices
Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomaterialia 2016, 46: 91-100. PMID: 27693690, PMCID: PMC5451113, DOI: 10.1016/j.actbio.2016.09.043.Peer-Reviewed Original ResearchConceptsExtracellular matrixLung extracellular matrixMatrix proteinsECM-associated proteinsCell-matrix interactionsProtein extraction methodsWhole organ regenerationRegenerative medicineOrganotypic cell culturesQuantitative proteomicsAcellular extracellular matrixECM proteinsOrgan regenerationCell adhesionProteomicsProtein analysisQuantitative biochemical dataProteinPotent substrateXenogeneic extracellular matrixTargeted proteomicsCell nuclear antigenBiochemical dataImportant glycoproteinCell cultures
2015
ENPP1-Fc prevents mortality and vascular calcifications in rodent model of generalized arterial calcification of infancy
Albright RA, Stabach P, Cao W, Kavanagh D, Mullen I, Braddock AA, Covo MS, Tehan M, Yang G, Cheng Z, Bouchard K, Yu ZX, Thorn S, Wang X, Folta-Stogniew EJ, Negrete A, Sinusas AJ, Shiloach J, Zubal G, Madri JA, De La Cruz EM, Braddock DT. ENPP1-Fc prevents mortality and vascular calcifications in rodent model of generalized arterial calcification of infancy. Nature Communications 2015, 6: 10006. PMID: 26624227, PMCID: PMC4686714, DOI: 10.1038/ncomms10006.Peer-Reviewed Original ResearchConceptsChronic kidney diseaseVascular calcificationArterial calcificationOrphan diseaseCommon diseaseSequelae of diseaseEctopic vascular calcificationInternal elastic laminaPrevent mortalityRenal failureCardiac failureKidney diseaseSubcutaneous administrationRodent modelsAnimal modelsEctopic calcificationVascular wallLarge arteriesElastic laminaDiseaseCalcificationCalciphylaxisDecreased concentrationSclerosisArteryCD44 Influences Fibroblast Behaviors Via Modulation of Cell–Cell and Cell–Matrix Interactions, Affecting Survivin and Hippo Pathways
Tsuneki M, Madri JA. CD44 Influences Fibroblast Behaviors Via Modulation of Cell–Cell and Cell–Matrix Interactions, Affecting Survivin and Hippo Pathways. Journal Of Cellular Physiology 2015, 231: 731-743. PMID: 26248063, DOI: 10.1002/jcp.25123.Peer-Reviewed Original ResearchConceptsHippo pathwayN-cadherinCaspase-3Cell-matrix interactionsSiRNA knock-downCollagen type IPivotal roleNon-coated dishesKnock-downExtracellular matrix expressionHigh cell densitySiRNA knockdownCell adhesionCell behaviorCell typesDiverse arrayPhospho-YAPNuclear fractionRole of CD44Fibroblast migrationFibroblast behaviorType IMesenchymal tissuePathwayCells cellsModulation of Sox10, HIF-1α, Survivin, and YAP by Minocycline in the Treatment of Neurodevelopmental Handicaps following Hypoxic Insult
Li Q, Tsuneki M, Krauthammer M, Couture R, Schwartz M, Madri JA. Modulation of Sox10, HIF-1α, Survivin, and YAP by Minocycline in the Treatment of Neurodevelopmental Handicaps following Hypoxic Insult. American Journal Of Pathology 2015, 185: 2364-2378. PMID: 26209807, PMCID: PMC5801488, DOI: 10.1016/j.ajpath.2015.05.016.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsApoptosisCell Cycle ProteinsDisease Models, AnimalHypoxiaHypoxia-Inducible Factor 1, alpha SubunitInhibitor of Apoptosis ProteinsMice, Inbred C57BLMinocyclineMultiple SclerosisPhosphoproteinsRepressor ProteinsSOXE Transcription FactorsSurvivinUp-RegulationYAP-Signaling ProteinsConceptsMinocycline treatmentNeurodevelopmental handicapHypoxic insultEffects of minocyclineUntoward side effectsAnimal model studiesPotential therapeutic targetSublethal hypoxic conditionsPremature infantsMultiple sclerosisCurrent therapiesTreatment trialsChronic hypoxiaSynaptic transmissionMurine modelMouse pupsMotor handicapNewborn populationSide effectsTherapeutic targetSublethal hypoxiaHIF-1αNerve transmissionMinocyclineCognitive functionA hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway
Tsuneki M, Hardee S, Michaud M, Morotti R, Lavik E, Madri JA. A hydrogel-endothelial cell implant mimics infantile hemangioma: modulation by survivin and the Hippo pathway. Laboratory Investigation 2015, 95: 765-780. PMID: 25961170, PMCID: PMC4828971, DOI: 10.1038/labinvest.2015.61.Peer-Reviewed Original ResearchAdaptor Proteins, Signal TransducingAnimalsCell Cycle ProteinsCells, CulturedChildChild, PreschoolDisease Models, AnimalEndothelial CellsFemaleHemangiomaHumansHydrogel, Polyethylene Glycol DimethacrylateInfantInhibitor of Apoptosis ProteinsLIM Domain ProteinsMacrophagesMaleMice, Inbred C57BLPhosphoproteinsRepressor ProteinsSurvivinTissue Array AnalysisTissue ScaffoldsYAP-Signaling ProteinsCell–extracellular matrix interactions in oral tumorigenesis: Roles of podoplanin and CD44 and modulation of Hippo pathway
Tsuneki M, Madri J, Saku T. Cell–extracellular matrix interactions in oral tumorigenesis: Roles of podoplanin and CD44 and modulation of Hippo pathway. Journal Of Oral Biosciences 2015, 57: 45-53. DOI: 10.1016/j.job.2015.02.004.Peer-Reviewed Original ResearchCell-extracellular matrix interactionsHippo pathwayExtracellular matrixDynamic reciprocal interactionsMatrix interactionsCell surfaceHyaluronan-rich extracellular matrixMultifunctional transmembrane proteinBasolateral cell surfaceVascular endothelialEndothelial cell survivalAdhesion moleculesStromal cellsVascular endothelial cell survivalTransmembrane proteinMurine hemangioendothelioma cellsTumor cellsSquamous cell carcinoma cellsOral squamous cell carcinoma cellsCell survivalContact inhibitionVE-cadherinTissue architectureTumor survivalIntercellular spaces