1996
In vitro inhibition of cell proliferation, viability, and invasiveness in U87MG human glioblastoma cells by estramustine phosphate.
Yoshida D, Piepmeier J, Teramoto A. In vitro inhibition of cell proliferation, viability, and invasiveness in U87MG human glioblastoma cells by estramustine phosphate. Neurosurgery 1996, 39: 360-6. PMID: 8832674, DOI: 10.1097/00006123-199608000-00025.Peer-Reviewed Original ResearchConceptsEstramustine phosphateMumol/LGlioblastoma cell linesCell proliferationRelative survival ratesTreatment of glioblastomaCell linesTime-dependent depressionAnti-invasive abilityDevelopment of agentsHuman glioblastoma cell linesU87MG human glioblastoma cellsHuman glioblastoma cellsSurvival rateTumor proliferationDrug concentrationsMonotetrazolium assayAntiproliferative capacityCell invasivenessGlioblastoma cellsNon-DNA targetsBasement membraneInvasion indexInvasive potentialSelective antiproliferative activity
1994
Selective antimitotic effects of estramustine correlate with its antimicrotubule properties on glioblastoma and astrocytes.
Yoshida D, Cornell-Bell A, Piepmeier J. Selective antimitotic effects of estramustine correlate with its antimicrotubule properties on glioblastoma and astrocytes. Neurosurgery 1994, 34: 863-7; discussion 867-8. PMID: 8052384, DOI: 10.1227/00006123-199405000-00012.Peer-Reviewed Original ResearchConceptsAntimitotic effectHuman glioblastoma cellsGlioma cellsGlioblastoma cellsEstramustine binding proteinModest antiproliferative effectsConcentration-dependent cytotoxic effectConcentration-dependent inhibitionEstramustine treatmentImmunohistochemical analysisAstrocyte viabilityEstramustineAstrocyte culturesAntiproliferative effectsMonoclonal antibodiesAstrocytesHuman glioblastomaTumor culturesCytotoxic effectsDimethylthiazol-2Diphenyltetrazolium bromideAntimicrotubule activityDeoxyribonucleic acid synthesisEstramustine sensitizes human glioblastoma cells to irradiation.
Yoshida D, Piepmeier J, Weinstein M. Estramustine sensitizes human glioblastoma cells to irradiation. Cancer Research 1994, 54: 1415-7. PMID: 8137240.Peer-Reviewed Original ResearchConceptsMicroM estramustineRadiation enhancerGlioma cellsEffect of estramustineGlioblastoma cellsG2M cellsConcentration-dependent inhibitionMalignant glioma cellsHuman glioma cellsMinimal systemic toxicityEstramustineHuman glioblastoma cellsPotentiation factorSystemic toxicityG2M fractionFlow cytometryClonogenic survivalAntimicrotubule agentsCytotoxic effectsControl cellsH treatmentDaily scheduleCellsCell cycleRadiation sensitivity