2023
THU313 Lactation As A Possible Protective Factor For Postpartum Diabetes In Women With Gestational Diabetes Mellitus: A Retrospective Analysis
Dávila-Parrilla V, Stanton C, Hackerson C, Song H, Hens J, Wysolmerski J, Merriam A, De Aguiar R. THU313 Lactation As A Possible Protective Factor For Postpartum Diabetes In Women With Gestational Diabetes Mellitus: A Retrospective Analysis. Journal Of The Endocrine Society 2023, 7: bvad114.747. PMCID: PMC10553496, DOI: 10.1210/jendso/bvad114.747.Peer-Reviewed Original ResearchGestational diabetes mellitusYale-New Haven HospitalInfant feeding practicesExclusive lactationFeeding practicesDiabetes mellitusRetrospective analysisOral glucose tolerance test resultsGlucose tolerance test resultsSignificant differencesExclusive formula feedingWeek postpartum visitPre-pregnancy BMITolerance test resultsDevelopment of T2DMPre-diabetic statusPopulation of womenNew Haven HospitalAfrican AmericansPossible protective factorDM statusPostpartum visitChart reviewFormula feedingDietary modificationTHU302 Protective Effects Of Lactation On Maternal Metabolism
Hens J, Ding Y, Brown S, Song H, Wysolmerski J, De Aguiar R. THU302 Protective Effects Of Lactation On Maternal Metabolism. Journal Of The Endocrine Society 2023, 7: bvad114.737. PMCID: PMC10554876, DOI: 10.1210/jendso/bvad114.737.Peer-Reviewed Original ResearchNL miceGlucose metabolismL miceBody compositionEuglycemic hyperinsulinemic clamp techniquePlasma free fatty acidsHistory of lactationMaternal glucose metabolismTissue triglyceride levelsGlucose tolerance testType 2 diabetesEuglycemic hyperinsulinemic clampGlucose infusion rateAge-matched cohortNumber of isletsAge-matched virginsLiver triglyceridesInsulin levelsTriglyceride levelsLiver weightTolerance testInsulin secretionMaternal metabolismFree fatty acidsInfusion rate
2022
Hyperinsulinemia induces early and dyssynchronous puberty in lean female mice.
Saleh FL, Joshi AA, Tal A, Xu P, Hens J, Wong SL, Flannery C. Hyperinsulinemia induces early and dyssynchronous puberty in lean female mice. Journal Of Endocrinology 2022, 254: 121-135. PMID: 35904489, PMCID: PMC9837806, DOI: 10.1530/joe-21-0447.Peer-Reviewed Original ResearchConceptsVaginal openingInsulin resistanceDay of VOIGF-1 levelsInsulin-like growth factor 1 receptorGreater insulin resistanceHigher insulin levelsEffect of hyperinsulinemiaGrowth factor 1 receptorGonadotropin-releasing hormoneLower body weightFactor 1 receptorReceptor isoform expressionMammary gland developmentLH levelsInsulin levelsInsulin receptor isoform expressionKisspeptin expressionChildhood obesityFemale miceHormone levelsEarly initiationHyperinsulinemiaBody weightOvarian folliclesPTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development
Grinman DY, Boras-Granic K, Takyar FM, Dann P, Hens JR, Marmol C, Lee J, Choi J, Chodosh LA, Sola MEG, Wysolmerski JJ. PTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development. Breast Cancer Research 2022, 24: 30. PMID: 35440032, PMCID: PMC9020078, DOI: 10.1186/s13058-022-01523-1.Peer-Reviewed Original ResearchConceptsOverexpression of PTHrPSecretory differentiationEpithelial cellsPTHrP overexpressionTumor latencyBreast cancerMammary epithelial cellsType 1 PTH/PTHrP receptorMMTV-PyMT breast cancer modelPTH/PTHrP receptorMMTV-PyMT miceMammary tumor developmentBreast cancer riskBreast cancer biologyExpression of PTHrPHormone-related proteinHuman breast cancerBreast cancer modelE74-like factor 5Normal mammary epithelial cellsBreast cancer progressionMouse mammary tumorsExpression of markersAlveolar epithelial cellsLuminal epithelial cells
2019
NHERF1 Is Required for Localization of PMCA2 and Suppression of Early Involution in the Female Lactating Mammary Gland
Jeong J, Kim W, Hens J, Dann P, Schedin P, Friedman PA, Wysolmerski JJ. NHERF1 Is Required for Localization of PMCA2 and Suppression of Early Involution in the Female Lactating Mammary Gland. Endocrinology 2019, 160: 1797-1810. PMID: 31087002, PMCID: PMC6619491, DOI: 10.1210/en.2019-00230.Peer-Reviewed Original ResearchConceptsPlasma membrane calcium ATPase 2Mammary epithelial cellsSpecialized plasma membrane domainsMammary epithelial cell deathLuminal epithelial cellsCell deathPremature mammary gland involutionBreast cancer cellsApical-basal polarityEpithelial cellsPlasma membrane domainsApical membraneSecretory luminal epithelial cellsExchanger regulatory factor 1Cell death pathwaysNHERF1 expressionMammary gland involutionCancer cellsRegulatory factor 1Sodium-hydrogen exchanger regulatory factor-1Lactating Mammary GlandNormal mammary epithelial cellsMembrane localizationEpithelial cell deathMembrane domains
2017
METHIONINE RESTRICTION ALTERS HEPATIC MIRNAS INVOLVED IN METABOLISM IN YOUNG, OBESE, AND AGED MICE
Park M, Cooke D, Plummer J, Ables G, Hens J. METHIONINE RESTRICTION ALTERS HEPATIC MIRNAS INVOLVED IN METABOLISM IN YOUNG, OBESE, AND AGED MICE. Innovation In Aging 2017, 1: 857-857. PMCID: PMC6184731, DOI: 10.1093/geroni/igx004.3084.Peer-Reviewed Original ResearchDIO miceAged miceMethionine restrictionYoung miceNonalcoholic fatty liver diseaseDiet-induced obese miceMiR-33-5pFatty liver conditionFatty liver diseaseDietary methionine restrictionType II diabetesExpression of mRNAAge-associated disordersBile acid transportLiver diseaseLiver functionObese miceLet-7gLiver conditionsMR dietOld miceHepatic expressionTransport of cholesterolBile acidsII diabetes
2016
Erratum to: Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice
Hens J, Sinha I, Perodin F, Cooper T, Sinha R, Plummer J, Perrone C, Orentreich D. Erratum to: Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice. BMC Cancer 2016, 16: 474. PMID: 27417544, PMCID: PMC4944442, DOI: 10.1186/s12885-016-2404-0.Peer-Reviewed Original ResearchMethionine‐Restricted Diet Increases miRNAs That Can Target RUNX2 Expression and Alters Bone Structure in Young Mice
Plummer J, Park M, Perodin F, Horowitz MC, Hens JR. Methionine‐Restricted Diet Increases miRNAs That Can Target RUNX2 Expression and Alters Bone Structure in Young Mice. Journal Of Cellular Biochemistry 2016, 118: 31-42. PMID: 27191548, PMCID: PMC5426510, DOI: 10.1002/jcb.25604.Peer-Reviewed Original ResearchConceptsDietary methionine restrictionBone tissue densityMR miceMethionine restrictionBone marrowMale C57BL/6J miceBone-related biomarkersYoung male C57BL/6J miceBone structureMiR-335-5pPlasma levelsC57BL/6J micePlasma glucoseRodent modelsBone volumeFat depotsTrabecular thicknessFat accretionBone remodelingMiceBiomechanical testingMiR-204Reduced expressionMiR-31Significant decreaseMethionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice
Hens JR, Sinha I, Perodin F, Cooper T, Sinha R, Plummer J, Perrone CE, Orentreich D. Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell cycle inhibitors in athymic nude mice. BMC Cancer 2016, 16: 349. PMID: 27255182, PMCID: PMC4891836, DOI: 10.1186/s12885-016-2367-1.Peer-Reviewed Original ResearchConceptsMethionine restrictionMR miceMR dietCell cycle inhibitorsNude micePlasma amino acid concentrationsBreast cancer xenograft modelCancer xenograft modelAthymic nude miceBreast cancer cell linesTumors of miceCycle inhibitorsMDA-MB-231 cellsConventional cancer therapiesAqueous One Solution Cell ProliferationCell Titer 96MDA-MB-231Cancer cell linesProgression of cancerInsulin sensitivityMammary gland tissuePlasma concentrationsMammary tumorsSmall tumorsAmino acid concentrationsMethionine restriction beyond life‐span extension
Ables GP, Hens JR, Nichenametla SN. Methionine restriction beyond life‐span extension. Annals Of The New York Academy Of Sciences 2016, 1363: 68-79. PMID: 26916321, DOI: 10.1111/nyas.13014.Peer-Reviewed Original ResearchConceptsMethionine restrictionLife span extensionPossible downstream effectorsMitochondrial oxidative stressAge-related diseasesCystathionine β-synthaseIntracellular regulatory mechanismsEpigenetic mechanismsNoncoding RNAsDownstream effectorsSpecific genesReactive oxygen speciesRegulatory mechanismsCell cycleBody sizeMethionine cycleEpigeneticsCancer progressionΒ-synthaseDietary methionine restrictionCell apoptosisFactor 1Fibroblast growth factor 21Hepatic glucose metabolismInsulin-like growth factor-1
2012
Parathyroid hormone-related protein activates Wnt signaling to specify the embryonic mammary mesenchyme
Hiremath M, Dann P, Fischer J, Butterworth D, Boras-Granic K, Hens J, Van Houten J, Shi W, Wysolmerski J. Parathyroid hormone-related protein activates Wnt signaling to specify the embryonic mammary mesenchyme. Development 2012, 139: 4239-4249. PMID: 23034629, PMCID: PMC3478689, DOI: 10.1242/dev.080671.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta CateninCell DifferentiationFemaleGene Expression Regulation, DevelopmentalKeratinocytesLymphoid Enhancer-Binding Factor 1Mammary Glands, AnimalMesodermMiceMice, KnockoutParathyroid Hormone-Related ProteinReceptors, Parathyroid HormoneThrombospondinsWnt ProteinsWnt Signaling PathwayConceptsLoss of PTHrPOverexpression of PTHrPHormone-related proteinMammary mesenchymeΒ-cateninEmbryonic mammary mesenchymeWnt pathwayWnt/β-cateninEmbryonic mammary developmentCanonical Wnt pathwayPTHrPMammary developmentMammary budAbnormal differentiationReduced expressionBasal keratinocytesVentral skinReporter activityBud cellsMarkersCanonical WntInappropriate differentiationAbolished expressionMesenchyme markersOverexpressionThe role and function of cadherins in the mammary gland
Andrews JL, Kim AC, Hens JR. The role and function of cadherins in the mammary gland. Breast Cancer Research 2012, 14: 203. PMID: 22315958, PMCID: PMC3496113, DOI: 10.1186/bcr3065.Peer-Reviewed Original ResearchConceptsMesenchymal-epithelial transitionEpithelial-mesenchymal transitionFunction of cadherinsMammary glandMechanism of regulationCell-cell contactPartial epithelial-mesenchymal transitionMetastatic cancer cellsLarge superfamilyNormal physiological conditionsTransmembrane receptorsCell motilityHeterophilic interactionsCadherinCadherin expressionCancer cellsMesenchymal markersPhysiological conditionsEpithelial cellsCurrent understandingRegulationMotilityDifferent organ systemsExpressionProliferation
2011
Localization of CTGF in mouse embryonic mammary gland development
Sambamurty A, Kim A, Barkley T, Hens J. Localization of CTGF in mouse embryonic mammary gland development. Developmental Biology 2011, 356: 144. DOI: 10.1016/j.ydbio.2011.05.148.Peer-Reviewed Original ResearchRegulation and expression of CTGF during adult mammary gland morphogenesis
Kim A, Sambamurty A, Barkley T, Hens J. Regulation and expression of CTGF during adult mammary gland morphogenesis. Developmental Biology 2011, 356: 144. DOI: 10.1016/j.ydbio.2011.05.149.Peer-Reviewed Original Research
2010
Cadherin-11 functions during mammary gland branching morphogenesis
Hens J, Kumar A, Nuttall J, Sanyal N, Vos M. Cadherin-11 functions during mammary gland branching morphogenesis. Developmental Biology 2010, 344: 446. DOI: 10.1016/j.ydbio.2010.05.137.Peer-Reviewed Original Research
2009
Analysis of gene expression in PTHrP−/− mammary buds supports a role for BMP signaling and MMP2 in the initiation of ductal morphogenesis
Hens J, Dann P, Hiremath M, Pan T, Chodosh L, Wysolmerski J. Analysis of gene expression in PTHrP−/− mammary buds supports a role for BMP signaling and MMP2 in the initiation of ductal morphogenesis. Developmental Dynamics 2009, 238: 2713-2724. PMID: 19795511, PMCID: PMC2862621, DOI: 10.1002/dvdy.22097.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 4Cells, CulturedDipeptidesDown-RegulationGene Expression Regulation, DevelopmentalHydroxamic AcidsIntercellular Signaling Peptides and ProteinsKeratinsMammary Glands, AnimalMatrix Metalloproteinase 2Matrix Metalloproteinase InhibitorsMesodermMiceMice, KnockoutMorphogenesisOligonucleotide Array Sequence AnalysisParathyroid Hormone-Related ProteinProtease InhibitorsSignal TransductionTranscription FactorsUp-RegulationConceptsGene expressionMammary budMammary mesenchymeDuctal outgrowthMesenchymal cellsEmbryonic mammary developmentMMP2 gene expressionEmbryonic mammary budFunctional roleGenesDuctal morphogenesisBud culturesMammary developmentBudsPTHrP effectsBMPMesenchymeExpressionMMP2 activityMMP2OutgrowthVentral skinCellsMorphogenesisBMP4
2008
Preservation of β-Galactosidase Transgene in Decalcified Murine Bone Specimens Embedded in Paraffin
Dann P, Hens J, Troiano N, Wysolmerski J, Kacena M. Preservation of β-Galactosidase Transgene in Decalcified Murine Bone Specimens Embedded in Paraffin. Journal Of Histotechnology 2008, 31: 61-64. DOI: 10.1179/his.2008.31.2.61.Peer-Reviewed Original ResearchPreservation of b-galactosidase transgene in decalcified murine bone specimens embedded in paraffin
Dann P, Hens JR, Troiano NW, Wysolmerski J, Kacena MA. Preservation of b-galactosidase transgene in decalcified murine bone specimens embedded in paraffin. J Histotech 31(2): 61-64, 2008Peer-Reviewed Original Research
2007
BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction
Hens JR, Dann P, Zhang JP, Harris S, Robinson GW, Wysolmerski J. BMP4 and PTHrP interact to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Development 2007, 134: 1221-1230. PMID: 17301089, DOI: 10.1242/dev.000182.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Morphogenetic Protein 4Bone Morphogenetic Protein Receptors, Type IBone Morphogenetic ProteinsDNA-Binding ProteinsEmbryo, MammalianGene Expression Regulation, DevelopmentalHair FollicleHomeodomain ProteinsMammary Glands, AnimalMesodermMiceMice, Mutant StrainsParathyroid Hormone-Related ProteinRNA, MessengerSignal TransductionUp-RegulationConceptsMammary mesenchymeBMP signalingMammary budMesenchymal cellsMammary epithelial cell fateEpithelial cell fateParathyroid hormone-related proteinHair follicle inductionEmbryonic mammary developmentMammary bud formationHormone-related proteinHair follicle formationMammary epithelial cellsMsx2 gene expressionCell fateEmbryonic epidermisMsx2 geneMsx2 expressionMammary placodesMouse embryosGene expressionVentral epidermisDuctal developmentBMP4Bud formation
2006
Initial Characterization of PTH‐Related Protein Gene‐Driven lacZ Expression in the Mouse*
Chen X, Macica CM, Dreyer BE, Hammond VE, Hens JR, Philbrick WM, Broadus AE. Initial Characterization of PTH‐Related Protein Gene‐Driven lacZ Expression in the Mouse*. Journal Of Bone And Mineral Research 2006, 21: 113-123. PMID: 16355280, DOI: 10.1359/jbmr.051005.Peer-Reviewed Original Research