Featured Publications
Single-cell microRNA-mRNA co-sequencing reveals non-genetic heterogeneity and mechanisms of microRNA regulation
Wang N, Zheng J, Chen Z, Liu Y, Dura B, Kwak M, Xavier-Ferrucio J, Lu YC, Zhang M, Roden C, Cheng J, Krause DS, Ding Y, Fan R, Lu J. Single-cell microRNA-mRNA co-sequencing reveals non-genetic heterogeneity and mechanisms of microRNA regulation. Nature Communications 2019, 10: 95. PMID: 30626865, PMCID: PMC6327095, DOI: 10.1038/s41467-018-07981-6.Peer-Reviewed Original ResearchConceptsSame single cellMicroRNA-mRNASingle cellsGenome-scale analysisNon-genetic cellNon-genetic heterogeneityMultiple omic profilesGenomic approachesMicroRNA regulationMolecular regulationTarget mRNAsExpression variabilityCellular pathwaysRegulatory relationshipsLevels of microRNAsIntercellular heterogeneityOmics profilesIntercellular variabilityCell heterogeneityMRNA profilesMicroRNAsMRNACellsRegulationExpression
2019
Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State
Chen Z, Lu Y, Zhang K, Xiao Y, Lu J, Fan R. Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State. Advanced Science 2019, 6: 1801361. PMID: 31065513, PMCID: PMC6498135, DOI: 10.1002/advs.201801361.Peer-Reviewed Original ResearchImmune cellsLigand lipopolysaccharideTime pointsToll-like receptor 4 ligand lipopolysaccharideBasal stateIndividual immune cellsActivation stateHuman macrophage responseEffector responsesMultiple time pointsInflammatory programSingle-cell RNA sequencingMacrophage responsePathogenic challengeSecretion assaysCell populationsSame single cellTime courseHomogeneous cell populationSecretion analysisLongitudinal trackingRNA sequencingCellsHeterogeneous responseSequential measurements