2018
Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment
Bonello TT, Perez-Vale KZ, Sumigray KD, Peifer M. Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment. Development 2018, 145: dev157941. PMID: 29361565, PMCID: PMC5825837, DOI: 10.1242/dev.157941.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAnimalsAnimals, Genetically ModifiedCell PolarityDrosophila melanogasterDrosophila ProteinsFemaleGastrulationGene Knockdown TechniquesGuanine Nucleotide Exchange FactorsIntracellular Signaling Peptides and ProteinsMaleModels, BiologicalProtein Interaction Domains and MotifsProtein TransportRNA InterferenceShelterin ComplexTelomere-Binding ProteinsConceptsApical-basal polarityPolarity establishmentMembrane localizationEpithelial apical-basal polarityAdherens junction positioningBazooka/Par3Adherens junction assemblyMost animal tissuesSmall GTPase Rap1Polarity initiationApical retentionNascent junctionsRA domainJunction assemblyAdherens junctionsTricellular junctionsPolarized cellsGTPase Rap1Macromolecular assembliesApical activationRap1Spatial organizationAnimal tissuesMultiple mechanismsEstablishment model
2013
Rap1 and Canoe/afadin are essential for establishment of apical–basal polarity in the Drosophila embryo
Choi W, Harris NJ, Sumigray KD, Peifer M. Rap1 and Canoe/afadin are essential for establishment of apical–basal polarity in the Drosophila embryo. Molecular Biology Of The Cell 2013, 24: 945-963. PMID: 23363604, PMCID: PMC3608504, DOI: 10.1091/mbc.e12-10-0736.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAnimalsCell LineCell PolarityCell ShapeCytoskeletonDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianGreen Fluorescent ProteinsIntracellular Signaling Peptides and ProteinsMicroscopy, ConfocalModels, BiologicalMutationProtein Kinase CRap1 GTP-Binding ProteinsRNA InterferenceConceptsAtypical protein kinase CCanoe/AfadinPolarity establishmentPolarity cuesDrosophila embryosAdherens junctionsApical-basal cell polarityBazooka/Par3Apical-basal polarityAbsence of Rap1Columnar cell shapeSmall GTPase Rap1Protein kinase CCell polarityBazookaGTPase Rap1Protein networkRap1Cell shapeLinear pathwaySuperb modelKinase COrgan architectureAfadinCytoskeleton