2024
Redefining intestinal stemness: The emergence of a new ISC population
Li M, Sumigray K. Redefining intestinal stemness: The emergence of a new ISC population. Cell 2024, 187: 2900-2902. PMID: 38848673, DOI: 10.1016/j.cell.2024.04.021.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationHomeostasisHumansIntestinal MucosaIntestinesMiceReceptors, G-Protein-CoupledStem Cells
2023
Generation and Manipulation of Rat Intestinal Organoids.
Zagoren E, Santos A, Ameen N, Sumigray K. Generation and Manipulation of Rat Intestinal Organoids. Journal Of Visualized Experiments 2023 PMID: 37427951, DOI: 10.3791/65343.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationHumansIntestinal MucosaIntestinesJejunumMiceOrganoidsRatsStem Cells
2022
Maf family transcription factors are required for nutrient uptake in the mouse neonatal gut
Bara A, Chen L, Ma C, Underwood J, Moreci R, Sumigray K, Sun T, Diao Y, Verzi M, Lechler T. Maf family transcription factors are required for nutrient uptake in the mouse neonatal gut. Development 2022, 149 PMID: 36504079, PMCID: PMC10112929, DOI: 10.1242/dev.201251.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCell DifferentiationMaf Transcription FactorsMiceNutrientsProto-Oncogene Proteins c-mafTranscription FactorsConceptsNutrient uptakeTranscription factorsMaf family transcription factorsMajor transcriptional changesFamily transcription factorsLoss of Blimp1Transcription factor MafBMaf proteinsCell fateTranscriptional regulatorsTranscriptional changesRNA-seqMaster regulatorEnterocyte genesFatty acid oxidationGene expressionPeroxisome numberAdult intestineMetabolic pathwaysMolecular componentsSubsequent degradationMaf factorsC-MafSimilar defectsIntestinal enterocytes
2018
Morphogenesis and Compartmentalization of the Intestinal Crypt
Sumigray KD, Terwilliger M, Lechler T. Morphogenesis and Compartmentalization of the Intestinal Crypt. Developmental Cell 2018, 45: 183-197.e5. PMID: 29689194, PMCID: PMC5987226, DOI: 10.1016/j.devcel.2018.03.024.Peer-Reviewed Original ResearchConceptsRac1 null miceAdult mammalian intestineCell shape changesProgenitor cellsStem cell nicheGene networksCrypt morphogenesisCrypt progenitor cellsEssential regulatorMammalian intestineCell nicheGenetic analysisUnexpected roleApical constrictionNiche formationHemidesmosomal adhesionCrypt developmentTissue architectureMouse cryptsMorphogenesisAbsorptive villiNull miceIntestinal cryptsQuantitative morphometricsShape changes
2015
Chapter Twelve Cell Adhesion in Epidermal Development and Barrier Formation
Sumigray KD, Lechler T. Chapter Twelve Cell Adhesion in Epidermal Development and Barrier Formation. Current Topics In Developmental Biology 2015, 112: 383-414. PMID: 25733147, PMCID: PMC4737682, DOI: 10.1016/bs.ctdb.2014.11.027.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAnimalsCell AdhesionCell Adhesion MoleculesCell DifferentiationEpidermal CellsEpidermisHumansSignal TransductionConceptsEpidermal developmentAdhesion proteinsCell-cell adhesionCell-cell junctionsCell biological studiesCell adhesion proteinsNoncanonical roleComposite proteinsAdhesive functionBarrier formationGrowth controlCell adhesionTissue physiologyProteinBiological studiesJunctional systemEpidermisTransductionAdhesionStructural integrityDifferentiationPhysiologyRoleFunctionJunction
2012
Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation
Sumigray KD, Foote HP, Lechler T. Noncentrosomal microtubules and type II myosins potentiate epidermal cell adhesion and barrier formation. Journal Of Cell Biology 2012, 199: 513-525. PMID: 23091070, PMCID: PMC3483132, DOI: 10.1083/jcb.201206143.Peer-Reviewed Original ResearchConceptsReorganization of microtubulesAdherens junctionsNoncentrosomal microtubulesCortical microtubulesCell adhesionCell-cell junctionsMyosin II recruitmentType II myosinMost cell typesDisruption of microtubulesMicrotubule cytoskeletonCell cortexEpidermal cell adhesionMyosin IITight junction functionMyosin IIAEpidermal cellsPhysiological roleBarrier activityCell typesMicrotubulesJunction functionDifferentiating epidermisChemical barrierCell sheets
2011
Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis
Sumigray KD, Chen H, Lechler T. Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. Journal Of Cell Biology 2011, 194: 631-642. PMID: 21844209, PMCID: PMC3160577, DOI: 10.1083/jcb.201104009.Peer-Reviewed Original ResearchMeSH Keywords1-Alkyl-2-acetylglycerophosphocholine EsteraseAlpha CateninAnimalsCarrier ProteinsCell DifferentiationCell ProliferationCells, CulturedDesmoplakinsDesmosomesEpidermisFluorescent Antibody TechniqueKeratinocytesMiceMice, KnockoutMicrotubule-Associated ProteinsMicrotubulesPermeabilityProtein TransportRecombinant Fusion ProteinsTransfectionConceptsDesmosomal protein desmoplakinCortical microtubule organizationCentrosomal proteinsMicrotubule organizationCell cortexMicrotubule reorganizationCell-cell adhesion structuresPenetrant perinatal lethalityDramatic defectsDesmosome stabilityCytoskeletal networkAdhesion structuresPerinatal lethalityUnexpected roleSingle isoformDesmosomal componentsBarrier activityCell typesDesmosomal proteinsEpidermal differentiationKeratin filamentsIntermediate filamentsProteinLIS1Specific subset