2024
Redefining intestinal stemness: The emergence of a new ISC population
Li M, Sumigray K. Redefining intestinal stemness: The emergence of a new ISC population. Cell 2024, 187: 2900-2902. PMID: 38848673, DOI: 10.1016/j.cell.2024.04.021.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationHomeostasisHumansIntestinal MucosaIntestinesMiceReceptors, G-Protein-CoupledStem Cells
2023
Generation and Manipulation of Rat Intestinal Organoids.
Zagoren E, Santos A, Ameen N, Sumigray K. Generation and Manipulation of Rat Intestinal Organoids. Journal Of Visualized Experiments 2023 PMID: 37427951, DOI: 10.3791/65343.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationHumansIntestinal MucosaIntestinesJejunumMiceOrganoidsRatsStem CellsAntibiotic-induced microbial depletion enhances murine small intestinal epithelial growth in a serotonin-dependent manner
Salvi P, Shaughnessy M, Sumigray K, Cowles R. Antibiotic-induced microbial depletion enhances murine small intestinal epithelial growth in a serotonin-dependent manner. AJP Gastrointestinal And Liver Physiology 2023, 325: g80-g91. PMID: 37158470, DOI: 10.1152/ajpgi.00113.2022.Peer-Reviewed Original ResearchConceptsSerotonin activitySERT protein expressionSerotonin potentiationEpithelial proliferationSerotonin transporterEndogenous serotoninVillus heightMicrobial depletionIntestinal epithelial growthProtein expressionEpithelial growthSerotonin-dependent mechanismIntestinal villus heightSmall intestine resultsIntestinal surface areaSmall intestinal villiSmall intestinal cryptsIntestinal pathologyPara-chlorophenylalanineISC numbersSerotonin synthesisIntestinal homeostasisEpithelial expressionMouse modelAnimal models
2015
The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestine
Zhou K, Sumigray KD, Lechler T. The Arp2/3 complex has essential roles in vesicle trafficking and transcytosis in the mammalian small intestine. Molecular Biology Of The Cell 2015, 26: 1995-2004. PMID: 25833710, PMCID: PMC4472011, DOI: 10.1091/mbc.e14-10-1481.Peer-Reviewed Original ResearchConceptsArp2/3 complexVesicle traffickingCell type-specific phenotypesCortical F-actinF-actin assemblyEssential roleMammalian small intestineF-actin filamentsCell polarityEndolysosomal systemEssential functionsF-actinCortical poolCell migrationCultured cellsIntestinal developmentTraffickingIntestinal epitheliumPhenotypeWide arrayComplexesComplex functionsTranscytosisLipid absorptionTranscytosis of IgGEpigenetic control of intestinal barrier function and inflammation in zebrafish
Marjoram L, Alvers A, Deerhake ME, Bagwell J, Mankiewicz J, Cocchiaro JL, Beerman RW, Willer J, Sumigray KD, Katsanis N, Tobin DM, Rawls JF, Goll MG, Bagnat M. Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 2770-2775. PMID: 25730872, PMCID: PMC4352795, DOI: 10.1073/pnas.1424089112.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsUbiquitin-like proteinEpigenetic controlTumor necrosis factorEpigenetic repressionLoss of functionIntestinal epitheliumPrecise regulationInflammatory bowel diseasePromoter methylationBarrier functionChronic inflammationEpithelial cellsTNF inductionPotent cytokineImmune cell recruitmentIntestinal barrier functionCell sheddingTNFA expressionRing fingerProinflammatory stimuliProper responseExpressionHoming signalsCell recruitment
2012
Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium
Sumigray KD, Lechler T. Desmoplakin controls microvilli length but not cell adhesion or keratin organization in the intestinal epithelium. Molecular Biology Of The Cell 2012, 23: 792-799. PMID: 22238362, PMCID: PMC3290639, DOI: 10.1091/mbc.e11-11-0923.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell AdhesionDesmoplakinsDesmosomesIntestinal MucosaKeratinsMiceMice, KnockoutMicrovilliConceptsDesmosomal protein desmoplakinProper cell-cell adhesionCell adhesionSimple epitheliaCell adhesion structuresApical junctional regionCell-cell adhesionTissue-specific functionsFunction of desmosomesKeratin filament networkTight junctionsKeratin organizationAdherens junctionsTissue homeostasisIntestinal epitheliumAdhesion structuresFilament networkCanonical functionFilament localizationKeratin filamentsMicrovilli lengthMicrovillus structureStratified epitheliumDesmoplakinDesmosomes