2024
Redefining intestinal stemness: The emergence of a new ISC population
Li M, Sumigray K. Redefining intestinal stemness: The emergence of a new ISC population. Cell 2024, 187: 2900-2902. PMID: 38848673, DOI: 10.1016/j.cell.2024.04.021.Peer-Reviewed Original Research
2023
Generation and Manipulation of Rat Intestinal Organoids.
Zagoren E, Santos A, Ameen N, Sumigray K. Generation and Manipulation of Rat Intestinal Organoids. Journal Of Visualized Experiments 2023 PMID: 37427951, DOI: 10.3791/65343.Peer-Reviewed Original ResearchAntibiotic-induced microbial depletion enhances murine small intestinal epithelial growth in a serotonin-dependent manner
Salvi P, Shaughnessy M, Sumigray K, Cowles R. Antibiotic-induced microbial depletion enhances murine small intestinal epithelial growth in a serotonin-dependent manner. AJP Gastrointestinal And Liver Physiology 2023, 325: g80-g91. PMID: 37158470, DOI: 10.1152/ajpgi.00113.2022.Peer-Reviewed Original ResearchConceptsSerotonin activitySERT protein expressionSerotonin potentiationEpithelial proliferationSerotonin transporterEndogenous serotoninVillus heightMicrobial depletionIntestinal epithelial growthProtein expressionEpithelial growthSerotonin-dependent mechanismIntestinal villus heightSmall intestine resultsIntestinal surface areaSmall intestinal villiSmall intestinal cryptsIntestinal pathologyPara-chlorophenylalanineISC numbersSerotonin synthesisIntestinal homeostasisEpithelial expressionMouse modelAnimal models
2019
Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut
Park J, Levic DS, Sumigray KD, Bagwell J, Eroglu O, Block CL, Eroglu C, Barry R, Lickwar CR, Rawls JF, Watts SA, Lechler T, Bagnat M. Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut. Developmental Cell 2019, 51: 7-20.e6. PMID: 31474562, PMCID: PMC6783362, DOI: 10.1016/j.devcel.2019.08.001.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAnimalsApoptosis Regulatory ProteinsDietary ProteinsDisease Models, AnimalEnterocytesFemaleGastrointestinal MicrobiomeGene DeletionGene Expression Regulation, DevelopmentalIleumIntestinal AbsorptionIntestinesKwashiorkorLigandsLysosomesMaleMembrane ProteinsMiceReceptors, Cell SurfaceZebrafishZebrafish ProteinsConceptsFluid-phase endocytosisEndocytic machineryTrans-cellular transportLuminal protein digestionVertebrate gutLarval zebrafishCritical developmental stagesStomachless fishMolecular mechanismsVertebrate growthProtein uptakeDevelopmental stagesIntracellular digestionProtein digestionConditional deletionStunted growthIntestinal cellsOral acquisitionDab2Dietary proteinSevere protein malnutritionDigestive functionNeonatal mammalsProteinMalnutrition syndrome
2018
Morphogenesis and Compartmentalization of the Intestinal Crypt
Sumigray KD, Terwilliger M, Lechler T. Morphogenesis and Compartmentalization of the Intestinal Crypt. Developmental Cell 2018, 45: 183-197.e5. PMID: 29689194, PMCID: PMC5987226, DOI: 10.1016/j.devcel.2018.03.024.Peer-Reviewed Original ResearchConceptsRac1 null miceAdult mammalian intestineCell shape changesProgenitor cellsStem cell nicheGene networksCrypt morphogenesisCrypt progenitor cellsEssential regulatorMammalian intestineCell nicheGenetic analysisUnexpected roleApical constrictionNiche formationHemidesmosomal adhesionCrypt developmentTissue architectureMouse cryptsMorphogenesisAbsorptive villiNull miceIntestinal cryptsQuantitative morphometricsShape changes