1996
HIV-1 Reverse Transcriptase Resistance to Nonnucleoside Inhibitors †
Spence R, Anderson K, Johnson K. HIV-1 Reverse Transcriptase Resistance to Nonnucleoside Inhibitors †. Biochemistry 1996, 35: 1054-1063. PMID: 8547241, DOI: 10.1021/bi952058+.Peer-Reviewed Original ResearchConceptsMutant enzymesPre-steady-state techniquesSingle nucleotide incorporationWild-type complexMaximum incorporation rateNucleotide incorporationEnzyme complexDuplex DNAAffinity 2Cysteine mutationsTwo-step bindingWild-typeConformational changesDecreased affinityEnzymePresence of nevirapineInhibitor resistanceMutationsIncorporation rateY181C mutationWild-type RTReverse transcriptaseHIV-1NevirapineY181C
1992
Mechanism and fidelity of HIV reverse transcriptase.
Kati W, Johnson K, Jerva L, Anderson K. Mechanism and fidelity of HIV reverse transcriptase. Journal Of Biological Chemistry 1992, 267: 25988-25997. PMID: 1281479, DOI: 10.1016/s0021-9258(18)35706-5.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBinding SitesDeoxyribonucleotidesDNAEscherichia coliHIV Reverse TranscriptaseHIV-1KineticsModels, BiologicalMolecular Sequence DataNucleic Acid HeteroduplexesOligodeoxyribonucleotidesOligoribonucleotidesRecombinant ProteinsRNARNA-Directed DNA PolymeraseSubstrate SpecificityTemplates, GeneticConceptsRNA templateRNA/DNA heteroduplexesRNA cleavageDuplex DNAPre-steady state burstRate of DNA polymerizationDNA-dependent polymerasesRNA cleavage productsBinding of dNTPSingle nucleotide incorporationRibonuclease domainRNA-dependentVirus reverse transcriptasePublished crystal structureNucleotide incorporationNucleoside triphosphatesHuman immunodeficiency virus reverse transcriptaseDNTP complexDissociation of DNADNA polymerizationReverse transcriptaseDATPHeteroduplexCleavage productsRNA