2015
Phosphoenolpyruvate Is a Metabolic Checkpoint of Anti-tumor T Cell Responses
Ho PC, Bihuniak JD, Macintyre AN, Staron M, Liu X, Amezquita R, Tsui YC, Cui G, Micevic G, Perales JC, Kleinstein SH, Abel ED, Insogna KL, Feske S, Locasale JW, Bosenberg MW, Rathmell JC, Kaech SM. Phosphoenolpyruvate Is a Metabolic Checkpoint of Anti-tumor T Cell Responses. Cell 2015, 162: 1217-1228. PMID: 26321681, PMCID: PMC4567953, DOI: 10.1016/j.cell.2015.08.012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCD4-Positive T-LymphocytesEndoplasmic ReticulumGlycolysisHexokinaseImmunotherapyLymphocytes, Tumor-InfiltratingMelanomaMiceMonitoring, ImmunologicNFATC Transcription FactorsPhosphoenolpyruvateReceptors, Antigen, T-CellSarcoplasmic Reticulum Calcium-Transporting ATPasesSignal TransductionTransforming Growth Factor betaTumor MicroenvironmentConceptsAnti-tumor T cell responsesT cell responsesT cellsEffector functionsCell responsesTumor-reactive T cellsTumor-infiltrating T cellsPhosphoenolpyruvate carboxykinase 1Tumoricidal effector functionsTumor-specific CD4CD8 T cellsT cell activityMelanoma-bearing miceAerobic glycolysisActivated T cellsMetabolic checkpointTumor growthCell activityTumor microenvironmentNFAT SignalingMetabolic reprogrammingCarboxykinase 1Anabolic metabolismCellsATPase activity
2004
LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1
Yang X, Yu K, Hao Y, Li DM, Stewart R, Insogna KL, Xu T. LATS1 tumour suppressor affects cytokinesis by inhibiting LIMK1. Nature Cell Biology 2004, 6: 609-617. PMID: 15220930, DOI: 10.1038/ncb1140.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin Depolymerizing FactorsActomyosinAnimalsAnimals, NewbornAntibodiesCell CycleCell DivisionCells, CulturedDNA-Binding ProteinsFeedback, PhysiologicalFetusGiant CellsHeLa CellsHumansLim KinasesMiceMice, KnockoutMicrofilament ProteinsPhosphorylationPolymersProtein BindingProtein KinasesProtein Serine-Threonine KinasesRNA InterferenceConceptsTumor suppressorActomyosin contractile ringPhosphorylation of cofilinCytokinesis defectsCytoskeleton regulatorsAntibody microinjectionContractile ringActin polymerizationGene knockoutCytokinesisLIMK1LATS1Multinucleate cellsNegative modulationSuppressorDrosophilaCellsColocalizesCofilinMammals
1994
Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells.
Eielson C, Kaplan D, Mitnick MA, Paliwal I, Insogna K. Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells. Endocrinology 1994, 135: 1639-44. PMID: 7925127, DOI: 10.1210/endo.135.4.7925127.Peer-Reviewed Original ResearchEstrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells
Eielson C, Kaplan D, Mitnick M, Paliwal I, Insogna K. Estrogen modulates parathyroid hormone-induced fibronectin production in human and rat osteoblast-like cells. Endocrinology 1994, 135: 1639-1644. DOI: 10.1210/en.135.4.1639.Peer-Reviewed Original ResearchParathyroid hormone-related protein: evidence for isoform- and tissue-specific posttranslational processing.
Yang KH, dePapp AE, Soifer NE, Dreyer BE, Wu TL, Porter SE, Bellantoni M, Burtis WJ, Insogna KL, Broadus AE. Parathyroid hormone-related protein: evidence for isoform- and tissue-specific posttranslational processing. Biochemistry 1994, 33: 7460-9. PMID: 8003511, DOI: 10.1021/bi00189a054.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCHO CellsChromatography, AffinityChromatography, High Pressure LiquidCricetinaeCricetulusCulture Media, ConditionedHumansImmunohistochemistryMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinPeptide FragmentsProtein Processing, Post-TranslationalProteinsTumor Cells, CulturedConceptsHormone-related proteinCarboxy-terminal fragmentCell linesTissue-specific posttranslational processingRat insulinoma cellsDistinct paracrineHuman PTHrP geneHumoral hypercalcemiaMalignant tumorsAutocrine roleRIN cell linesMidregion fragmentPTHrP isoformsMost human cell linesNonmalignant tissuesPhysiological rationalePTHrP geneInsulinoma cellsChinese hamster ovary cellsHuman cell linesHamster ovary cellsSecretory formCellsOvary cellsPosttranslational processing
1990
THE PARATHYROID HORMONE-RELATED PROTEIN STIMULATES HUMAN OSTEOBLAST-LIKE CELLS TO SECRETE A 9,000 DALTON BONERESORBING PROTEIN
Morris CA, Mitnick ME, Weir EC, Horowitz M, Kreider BL, Insogna KL. THE PARATHYROID HORMONE-RELATED PROTEIN STIMULATES HUMAN OSTEOBLAST-LIKE CELLS TO SECRETE A 9,000 DALTON BONERESORBING PROTEIN. Endocrinology 1990, 126: 1783-1786. PMID: 2307127, DOI: 10.1210/endo-126-3-1783.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related proteinBone-resorbing activityHormone-related proteinBone resorptionPTH/Bone-resorbing cytokinesIL-1GM-CSFSingle fractionSequential reverse-phase HPLCII cellsUntreated cellsKD fractionHuman osteoblast-like cellsResorptionSize-exclusion HPLCNgeqOsteoblast-like cellsSpecific activityCellsKD proteinOsteoblastsReversed-phase HPLCCytokinesPGE2