2018
The contribution of cross-talk between the cell-surface proteins CD36 and CD47–TSP-1 in osteoclast formation and function
Koduru SV, Sun BH, Walker JM, Zhu M, Simpson C, Dhodapkar M, Insogna KL. The contribution of cross-talk between the cell-surface proteins CD36 and CD47–TSP-1 in osteoclast formation and function. Journal Of Biological Chemistry 2018, 293: 15055-15069. PMID: 30082316, PMCID: PMC6166722, DOI: 10.1074/jbc.ra117.000633.Peer-Reviewed Original ResearchConceptsOsteoclast formationTSP-1Competitive NO synthase inhibitorPro-osteoclastogenic effectAntibody-mediated blockadeNitroarginine methyl esterNO synthase inhibitorNitric Oxide SignalingPTH infusionDifferentiation 47Parathyroid hormoneBone resorptionFemale miceHypercalcemic responseOsteoclast maturationSynthase inhibitorResorptive activitySynthetic agonistsCD36Osteoclast progenitorsMiceInhibitory effectCD47OsteoclastsWT osteoblasts
2013
EBI2 guides osteoclast precursors to bone niches and regulates osteoclastogenesis and bone mass homeostasis. (P5099)
Nevius E, Horowitz M, Insogna K, Ishii M, Pereira J. EBI2 guides osteoclast precursors to bone niches and regulates osteoclastogenesis and bone mass homeostasis. (P5099). The Journal Of Immunology 2013, 190: 129.14-129.14. DOI: 10.4049/jimmunol.190.supp.129.14.Peer-Reviewed Original ResearchOsteoclast precursorsMature osteoclastsBone nicheBone surfaceBone lining cellsEstrogen deficiencyBone marrow nicheBone disordersEBI2Osteoclast differentiationBone mass homeostasisMonocytic precursorsOsteoclastsMarrow nicheBone outgrowthsGuidance cuesSynthesis enzymesHomeostasisDifferentiationCH25HOsteoporosisCYP7B1OsteoclastogenesisMice
2010
Osteoclasts Lacking Rac2 Have Defective Chemotaxis and Resorptive Activity
Itokowa T, Zhu ML, Troiano N, Bian J, Kawano T, Insogna K. Osteoclasts Lacking Rac2 Have Defective Chemotaxis and Resorptive Activity. Calcified Tissue International 2010, 88: 75-86. PMID: 21110188, PMCID: PMC3155765, DOI: 10.1007/s00223-010-9435-3.Peer-Reviewed Original ResearchConceptsTrabecular bone massBone massMature osteoclastsFemoral cortical thicknessTotal bone areaNumber of osteoclastsWild-type miceNormal tooth eruptionNumber of osteoblastsWild-type animalsBone resorptionCortical porosityActin ring formationCortical thicknessTooth eruptionOsteoclast functionResorptive activityDefective chemotaxisBone areaFemale animalsMiceOsteoclastsMigratory rateNormal sizeSignificant differences
2006
Colony-Stimulating Factor-1 Increases Osteoclast Intracellular pH and Promotes Survival via the Electroneutral Na/HCO3 Cotransporter NBCn1
Bouyer P, Sakai H, Itokawa T, Kawano T, Fulton CM, Boron WF, Insogna KL. Colony-Stimulating Factor-1 Increases Osteoclast Intracellular pH and Promotes Survival via the Electroneutral Na/HCO3 Cotransporter NBCn1. Endocrinology 2006, 148: 831-840. PMID: 17068143, DOI: 10.1210/en.2006-0547.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAlkaliesAmilorideAnimalsBicarbonatesCarbon DioxideCaspase InhibitorsCell SeparationCell SurvivalCells, CulturedEnzyme InhibitorsHydrogen-Ion ConcentrationIntracellular FluidMacrolidesMacrophage Colony-Stimulating FactorMiceMice, Inbred StrainsOsteoclastsProtonsRatsSodiumSodium-Bicarbonate SymportersConceptsProlong osteoclast survivalOsteoclast-like cellsColony-stimulating factor-1Osteoclast survivalCotransporter NBCn1Survival of osteoclastsHCO3- cotransporter NBCn1Caspase-8Inhibition of apoptosisExtracellular sodiumPromotes SurvivalOsteoclastsRT-PCRCellular alkalinizationSurvivalFactor 1Caspase-3NBCn1Intracellular pHCell survivalElectroneutral Na/Block activationPresent studySame extentFirst evidence
1997
Role of c‐Src in cellular events associated with colony‐stimulating factor‐1‐induced spreading in osteoclasts
Insogna K, Tanaka S, Neff L, Horne W, Levy J, Baron R. Role of c‐Src in cellular events associated with colony‐stimulating factor‐1‐induced spreading in osteoclasts. Molecular Reproduction And Development 1997, 46: 104-108. PMID: 8981371, DOI: 10.1002/(sici)1098-2795(199701)46:1<104::aid-mrd16>3.0.co;2-2.BooksMeSH KeywordsActininAnimalsBone MarrowBone Marrow CellsCalcitriolCell SizeCells, CulturedCytoskeletonFemaleHumansMacrophage Colony-Stimulating FactorMiceMicroscopy, ConfocalOsteoblastsOsteoclastsOsteoporosisPhosphatidylinositol 4,5-DiphosphatePhosphorylationProtein Processing, Post-TranslationalProto-Oncogene Proteins pp60(c-src)RatsSignal TransductionConceptsOsteoclast-like cellsNeonatal rat osteoclastsRat osteoclastsCSF-1 treatmentMonoclonal antibodiesC-fmsCSF-1C-SrcCellular eventsWestern blot analysisTime-dependent increaseMurine bone marrowBasal abnormalitiesBone marrowConfocal microscopic studiesPIP2 contentSignificant doseReceptor tyrosine kinasesOsteoclastsTyrosine kinase activityC-Src kinase activityCell-signaling eventsTargeted disruptionTreatmentIntrinsic tyrosine kinase activity