2000
Evidence for a functional association between phosphatidylinositol 3-kinase and c-src in the spreading response of osteoclasts to colony-stimulating factor-1.
Grey A, Chen Y, Paliwal I, Carlberg K, Insogna K. Evidence for a functional association between phosphatidylinositol 3-kinase and c-src in the spreading response of osteoclasts to colony-stimulating factor-1. Endocrinology 2000, 141: 2129-38. PMID: 10830300, DOI: 10.1210/endo.141.6.7480.Peer-Reviewed Original ResearchAndrostadienesAnimalsBinding SitesCell MovementChromonesEnzyme InhibitorsImmunosorbent TechniquesMacrophage Colony-Stimulating FactorMiceMorpholinesOsteoclastsPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsProlineProto-Oncogene Proteins pp60(c-src)RatsReceptor, Macrophage Colony-Stimulating FactorRegulatory Sequences, Nucleic AcidSrc Homology DomainsWortmannin
1997
Colony-stimulating factor-1 induces cytoskeletal reorganization and c-src-dependent tyrosine phosphorylation of selected cellular proteins in rodent osteoclasts.
Insogna KL, Sahni M, Grey AB, Tanaka S, Horne WC, Neff L, Mitnick M, Levy JB, Baron R. Colony-stimulating factor-1 induces cytoskeletal reorganization and c-src-dependent tyrosine phosphorylation of selected cellular proteins in rodent osteoclasts. Journal Of Clinical Investigation 1997, 100: 2476-2485. PMID: 9366562, PMCID: PMC508448, DOI: 10.1172/jci119790.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnimals, NewbornCell MovementCells, CulturedCytoskeletonKineticsMacrophage Colony-Stimulating FactorMolecular Sequence DataOsteoclastsPeptidesPhosphoproteinsPhosphorylationPhosphotyrosinePolymerase Chain ReactionProto-Oncogene Proteins pp60(c-src)RatsSrc-Family KinasesSubstrate SpecificityConceptsColony-stimulating factor-1CSF-1 treatmentC-SrcC-fmsCellular proteinsC-Src-dependent tyrosine phosphorylationGrb2-binding proteinSrc kinase activityFactor 1Normal cytoskeletal architectureCytoskeletal architectureSrc geneCytoskeletal reorganizationTyrosine phosphorylationKinase activityPhosphotyrosine stainingCellular eventsF-actinActin distributionTargeted disruptionProteinRapid cytoplasmicLike cellsPhosphorylationOsteoclast-like cellsRole of c‐Src in cellular events associated with colony‐stimulating factor‐1‐induced spreading in osteoclasts
Insogna K, Tanaka S, Neff L, Horne W, Levy J, Baron R. Role of c‐Src in cellular events associated with colony‐stimulating factor‐1‐induced spreading in osteoclasts. Molecular Reproduction And Development 1997, 46: 104-108. PMID: 8981371, DOI: 10.1002/(sici)1098-2795(199701)46:1<104::aid-mrd16>3.0.co;2-2.BooksMeSH KeywordsActininAnimalsBone MarrowBone Marrow CellsCalcitriolCell SizeCells, CulturedCytoskeletonFemaleHumansMacrophage Colony-Stimulating FactorMiceMicroscopy, ConfocalOsteoblastsOsteoclastsOsteoporosisPhosphatidylinositol 4,5-DiphosphatePhosphorylationProtein Processing, Post-TranslationalProto-Oncogene Proteins pp60(c-src)RatsSignal TransductionConceptsOsteoclast-like cellsNeonatal rat osteoclastsRat osteoclastsCSF-1 treatmentMonoclonal antibodiesC-fmsCSF-1C-SrcCellular eventsWestern blot analysisTime-dependent increaseMurine bone marrowBasal abnormalitiesBone marrowConfocal microscopic studiesPIP2 contentSignificant doseReceptor tyrosine kinasesOsteoclastsTyrosine kinase activityC-Src kinase activityCell-signaling eventsTargeted disruptionTreatmentIntrinsic tyrosine kinase activity