2008
Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone Remodeling
Garimella R, Tague SE, Zhang J, Belibi F, Nahar N, Sun BH, Insogna K, Wang J, Anderson HC. Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone Remodeling. Journal Of Histochemistry & Cytochemistry 2008, 56: 569-577. PMID: 18319273, PMCID: PMC2386763, DOI: 10.1369/jhc.2008.950394.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone Marrow CellsBone Morphogenetic ProteinsBone RemodelingCoculture TechniquesFemurImmunohistochemistryIn Situ HybridizationIsoenzymesMiceOsteoclastsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSkullTartrate-Resistant Acid PhosphataseTibiaConceptsBone morphogenetic proteinBMP-2Morphogenetic proteinsBMP-6 mRNAExpression of transcriptsMouse calvarial osteoblastsDifferentiation of osteoblastsBMP-4Osteoclast lysatesOrchestrated processIntercellular communicationReal-time PCRCalvarial osteoblastsBone marrow cellsSitu hybridizationDirect rolePossible direct roleProteinWestern blottingBone remodeling siteBone formationOsteoblastsAnabolic boneMarrow cellsExpression
1997
Role of c‐Src in cellular events associated with colony‐stimulating factor‐1‐induced spreading in osteoclasts
Insogna K, Tanaka S, Neff L, Horne W, Levy J, Baron R. Role of c‐Src in cellular events associated with colony‐stimulating factor‐1‐induced spreading in osteoclasts. Molecular Reproduction And Development 1997, 46: 104-108. PMID: 8981371, DOI: 10.1002/(sici)1098-2795(199701)46:1<104::aid-mrd16>3.0.co;2-2.BooksMeSH KeywordsActininAnimalsBone MarrowBone Marrow CellsCalcitriolCell SizeCells, CulturedCytoskeletonFemaleHumansMacrophage Colony-Stimulating FactorMiceMicroscopy, ConfocalOsteoblastsOsteoclastsOsteoporosisPhosphatidylinositol 4,5-DiphosphatePhosphorylationProtein Processing, Post-TranslationalProto-Oncogene Proteins pp60(c-src)RatsSignal TransductionConceptsOsteoclast-like cellsNeonatal rat osteoclastsRat osteoclastsCSF-1 treatmentMonoclonal antibodiesC-fmsCSF-1C-SrcCellular eventsWestern blot analysisTime-dependent increaseMurine bone marrowBasal abnormalitiesBone marrowConfocal microscopic studiesPIP2 contentSignificant doseReceptor tyrosine kinasesOsteoclastsTyrosine kinase activityC-Src kinase activityCell-signaling eventsTargeted disruptionTreatmentIntrinsic tyrosine kinase activity
1996
Colony stimulating factor‐1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone‐related protein
Weir E, Lowik C, Paliwal I, Insogna K. Colony stimulating factor‐1 plays a role in osteoclast formation and function in bone resorption induced by parathyroid hormone and parathyroid hormone‐related protein. Journal Of Bone And Mineral Research 1996, 11: 1474-1481. PMID: 8889847, DOI: 10.1002/jbmr.5650111014.Peer-Reviewed Original ResearchConceptsBone resorptionMature osteoclastsParathyroid hormoneOsteoclast precursorsFetal rat long bonesParathyroid hormone-related proteinResistant acid phosphatase-positive osteoclastsMitogenic activityFactor 1Hormone-related proteinOrgan cultureCSF-1Rat long bonesTreatment of boneSignificant increaseOrgan culture assayHistological examinationBone assayOsteoclast recruitmentOsteoclast formationRat osteoclastsColony stimulating factor 1CSF-1 productionPTHResorption
1993
Macrophage colony‐stimulating factor release and receptor expression in bone cells
Weir E, Horowitz M, Baron R, Centrella M, Kacinski B, Insogna K. Macrophage colony‐stimulating factor release and receptor expression in bone cells. Journal Of Bone And Mineral Research 1993, 8: 1507-1518. PMID: 8304053, DOI: 10.1002/jbmr.5650081214.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowBone Marrow CellsCell DivisionCells, CulturedColony-Forming Units AssayCulture Media, ConditionedGranulocyte-Macrophage Colony-Stimulating FactorMacrophage Colony-Stimulating FactorMacrophagesMaleOsteoblastsOsteoclastsParathyroid HormoneParathyroid Hormone-Related ProteinProteinsRatsRats, Sprague-DawleyRats, WistarReceptor, Macrophage Colony-Stimulating FactorTumor Cells, CulturedConceptsColony-stimulating factorColony-stimulating activityM-CSFBone resorptionReceptor expressionMature osteoclastsPreincubation of CMColony-stimulating factor releaseHormone-related peptideM-CSF receptor c-FmsMacrophage colony-stimulating factorOsteoblast-osteoclast interactionsM-CSF mRNA levelsBone marrow cellsCSF-1 receptor expressionRat bone marrow cellsReceptor c-fmsROS 17/2.8 cellsParacrine roleImmunohistochemical techniquesRat osteoblastsPTHrP treatmentMarrow cellsFactor releasePTH