2010
Targeted overexpression of Dkk1 in osteoblasts reduces bone mass but does not impair the anabolic response to intermittent PTH treatment in mice
Yao GQ, Wu JJ, Troiano N, Insogna K. Targeted overexpression of Dkk1 in osteoblasts reduces bone mass but does not impair the anabolic response to intermittent PTH treatment in mice. Journal Of Bone And Mineral Metabolism 2010, 29: 141-148. PMID: 20602130, PMCID: PMC3457021, DOI: 10.1007/s00774-010-0202-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesHumansIntercellular Signaling Peptides and ProteinsMiceMice, TransgenicOsteoblastsOsteoclastsParathyroid HormoneRatsReverse Transcriptase Polymerase Chain ReactionConceptsParathyroid hormonePTH treatmentBone massTg miceAnabolic responseDKK1 expressionSingle daily subcutaneous doseDaily subcutaneous doseBone formationIntermittent PTH treatmentPotent anabolic agentOverexpression of DKK1Number of osteoblastsSubcutaneous doseWT miceReal-time PCRSkeletal sitesDickkopf-1Anabolic agentsBody weightTransgenic miceHistomorphometric parametersHistomorphometric analysisTargeted overexpressionPrimary murine osteoblasts
2008
Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone Remodeling
Garimella R, Tague SE, Zhang J, Belibi F, Nahar N, Sun BH, Insogna K, Wang J, Anderson HC. Expression and Synthesis of Bone Morphogenetic Proteins by Osteoclasts: A Possible Path to Anabolic Bone Remodeling. Journal Of Histochemistry & Cytochemistry 2008, 56: 569-577. PMID: 18319273, PMCID: PMC2386763, DOI: 10.1369/jhc.2008.950394.Peer-Reviewed Original ResearchMeSH KeywordsAcid PhosphataseAnimalsBlotting, WesternBone Marrow CellsBone Morphogenetic ProteinsBone RemodelingCoculture TechniquesFemurImmunohistochemistryIn Situ HybridizationIsoenzymesMiceOsteoclastsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSkullTartrate-Resistant Acid PhosphataseTibiaConceptsBone morphogenetic proteinBMP-2Morphogenetic proteinsBMP-6 mRNAExpression of transcriptsMouse calvarial osteoblastsDifferentiation of osteoblastsBMP-4Osteoclast lysatesOrchestrated processIntercellular communicationReal-time PCRCalvarial osteoblastsBone marrow cellsSitu hybridizationDirect rolePossible direct roleProteinWestern blottingBone remodeling siteBone formationOsteoblastsAnabolic boneMarrow cellsExpression
2005
Multiple melanocortin receptors are expressed in bone cells
Zhong Q, Sridhar S, Ruan L, Ding KH, Xie D, Insogna K, Kang B, Xu J, Bollag RJ, Isales CM. Multiple melanocortin receptors are expressed in bone cells. Bone 2005, 36: 820-831. PMID: 15804492, DOI: 10.1016/j.bone.2005.01.020.Peer-Reviewed Original ResearchMeSH KeywordsAdrenocorticotropic HormoneAnimalsBase SequenceBlotting, NorthernBlotting, SouthernCyclic AMPDNA PrimersHumansImmunohistochemistryMiceOligonucleotide Array Sequence AnalysisOsteoblastsPro-OpiomelanocortinProtein BindingRatsReceptors, CorticotropinReceptors, MelanocortinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerThymidineConceptsMelanocortin receptorsBone turnoverOsteoclastic cellsReceptor familySteroid hormone secretionBone cellsMelanocortin receptor familyFamily of receptorsHormone secretionBeta-endorphinSpecific effectsPOMC fragmentsPOMC mRNACell subpopulationsAlpha betaGamma-MSHReceptorsPeptide hormonesPOMCG proteinsVivo dataBoneHormoneOsteoblastic cellsVariety of genes