High-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity
Lu Y, Chen JJ, Mu L, Xue Q, Wu Y, Wu PH, Li J, Vortmeyer AO, Miller-Jensen K, Wirtz D, Fan R. High-Throughput Secretomic Analysis of Single Cells to Assess Functional Cellular Heterogeneity. Analytical Chemistry 2013, 85: 2548-2556. PMID: 23339603, PMCID: PMC3589817, DOI: 10.1021/ac400082e.Peer-Reviewed Original ResearchMeSH KeywordsAntibodiesCell LineChemokinesCytokinesEnzyme-Linked Immunospot AssayHigh-Throughput Screening AssaysHumansIntercellular Signaling Peptides and ProteinsProteinsTissue Array AnalysisTumor Cells, CulturedConceptsCellular functionsSingle cellsCellular heterogeneityArray of proteinsMultiplexed protein detectionSecreted proteinsSecretomic analysisLow sample consumptionComplex biological samplesProtein secretionIndividual cellsPrimary cellsProtein detectionAssay platformHigh throughputCell linesSample consumptionStandardized data analysisEase of operationProteinBiological samplesCellsHuman healthInformative monitoringDistinct heterogeneity