1998
High-Efficiency Gene Transfer and Pharmacologic Selection of Genetically Engineered Human Keratinocytes
Deng H, Choate K, Lin Q, Khavari P. High-Efficiency Gene Transfer and Pharmacologic Selection of Genetically Engineered Human Keratinocytes. BioTechniques 1998, 25: 274-280. PMID: 9714888, DOI: 10.2144/98252gt02.Peer-Reviewed Original ResearchConceptsCutaneous gene deliveryGene delivery efficiencyEfficient retroviral gene transferGene transferFuture genetic therapiesTherapeutic genesGene deliveryDelivery efficiencyRetroviral gene transferRetroviral vectorsGenetic therapiesLow efficiencyProduction of populationsSomatic cellsEfficiencyHuman keratinocytesArrayGeneticallyBlasticidinTransferDeliveryEpidermolysis bullosaDelivery effortsCapabilityProduction
1997
Direct Cutaneous Gene Delivery in a Human Genetic Skin Disease
Choate K, Khavari P. Direct Cutaneous Gene Delivery in a Human Genetic Skin Disease. Human Gene Therapy 1997, 8: 1659-1665. PMID: 9322868, DOI: 10.1089/hum.1997.8.14-1659.Peer-Reviewed Original ResearchConceptsLamellar ichthyosisCutaneous gene deliveryHuman genetic skin diseaseFunctional abnormalitiesDeficient patientsTherapeutic goalsNude miceSkin diseasesIntact skinVivo injectionPlasmid injectionSuprabasal epidermisPatient's skinGenetic skin diseasesAccessible somatic tissueDisease tissuesGene delivery approachSkinTherapeutic gene transferInjectionRetroviral expression vectorDiseaseNaked DNAPartial restorationTissue locationSustainability of Keratinocyte Gene Transfer and Cell Survival In Vivo
Choate K, Khavari P. Sustainability of Keratinocyte Gene Transfer and Cell Survival In Vivo. Human Gene Therapy 1997, 8: 895-901. PMID: 9195211, DOI: 10.1089/hum.1997.8.8-895.Peer-Reviewed Original ResearchConceptsGene deliveryCutaneous gene deliveryTherapeutic gene deliveryRetroviral vectorsNormal gene expression levelsBeta-gal expressionMultilayer epidermisGene transferRetroviral long terminal repeatEpidermal progenitor cellsSpecies-specific antibodiesVector sequencesCellular promotersKeratinocyte transglutaminaseDeliveryMarker gene expressionAttractive sitesGene productionType VII collagenHuman keratinocytesEfficiencyHuman involucrinTerminal repeat