Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins
Stone K, Williams K. Reverse-Phase HPLC Separation of Enzymatic Digests of Proteins. Springer Protocols Handbooks 2009, 941-950. DOI: 10.1007/978-1-59745-198-7_102.Peer-Reviewed Original ResearchLaser desorption mass spectrometrySites of chemicalHigh pressureUltra-high pressureRelative elution positionsBroad peakDesorption mass spectrometryPowerful techniquePhase resultsMobile phase resultsReversed-phase HPLCEnzymatic digestsLC systemRelevant parametersMatrix-assisted laser desorption mass spectrometryMass spectrometric approachReversed-phase HPLC separationReversephase HPLCTotal hydrophobicitySpectrometric approachAqueous mixturesMass spectrometryVolatile solventsResolutionHPLC separationEnzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels
Stone K, Gulcicek E, Williams K. Enzymatic Digestion of Proteins in Solution and in SDS Polyacrylamide Gels. Springer Protocols Handbooks 2009, 905-917. DOI: 10.1007/978-1-59745-198-7_99.Peer-Reviewed Original ResearchProtein of interestSDS-polyacrylamide gelsMass spectrometryGel digestion procedureChemical sequencingLaser desorption ionization mass spectrometryDesorption ionization mass spectrometryElectrospray ionization sourceIonization mass spectrometryComplex biological mixturesMass spectral acquisitionProtein identification analysisTandem mass spectrometrySDS-PAGEMass spectrometric analysisPolyacrylamide gelsIonization sourceNumerous genomesBiological mixturesPeptide mass dataProtein databaseCleavage procedureMass spectrometerGel matrixLC system