Clinical Application of cDNA Microarrays in Oncology
Pusztai L, Ayers M, Stec J, Hortobágyi GN. Clinical Application of cDNA Microarrays in Oncology. The Oncologist 2003, 8: 252-258. PMID: 12773747, DOI: 10.1634/theoncologist.8-3-252.Peer-Reviewed Original ResearchConceptsHundreds of genesGene expression patternsSingle-gene markersIndividual genesTranscriptional profilingCDNA microarrayMRNA speciesDNA microarraysExpression patternsComplex biologyNovel targetImportant new toolMicroarrayClinical outcomesGenesHuman tissuesImportant clinical outcomesDiseased tissuesDrug developmentTrue clinical utilityClinical utilityClinical OncologyNew toolBiologyExciting new technologyTotal RNA yield and microarray gene expression profiles from fine‐needle aspiration biopsy and core‐needle biopsy samples of breast carcinoma
Symmans WF, Ayers M, Clark EA, Stec J, Hess KR, Sneige N, Buchholz TA, Krishnamurthy S, Ibrahim NK, Buzdar AU, Theriault RL, Rosales MF, Thomas ES, Gwyn KM, Green MC, Syed AR, Hortobagyi GN, Pusztai L. Total RNA yield and microarray gene expression profiles from fine‐needle aspiration biopsy and core‐needle biopsy samples of breast carcinoma. Cancer 2003, 97: 2960-2971. PMID: 12784330, DOI: 10.1002/cncr.11435.Peer-Reviewed Original ResearchConceptsGene expression profilesTranscriptional profilesExpression profilesFine-needle aspiration biopsyGenomic databasesStromal gene expressionGene expressionTotal RNA yieldTotal RNABreast carcinomaTumor cell populationSubset of genesCDNA microarray analysisStromal cellsBiopsy samplesGene expression profilingCell populationsMicroscopic cell countsRNA yieldAspiration biopsyGenomic studiesTranscriptional profilingCDNA microarrayNonlymphoid stromal cellsExpression profilingMolecular profiles of invasive mucinous and ductal carcinomas of the breast a molecular case study
Pusztai L, Sotiriou C, Buchholz TA, Meric F, Symmans WF, Esteva FJ, Sahin A, Liu ET, Hortobagi GN. Molecular profiles of invasive mucinous and ductal carcinomas of the breast a molecular case study. Cancer Genetics 2003, 141: 148-153. PMID: 12606133, DOI: 10.1016/s0165-4608(02)00737-9.Peer-Reviewed Original ResearchConceptsExpression profilingComprehensive genetic analysisExpression of enzymesCDNA microarrayGenetic analysisMolecular case studyExpression profilesInhibitory genesNovel pathwayTumor phenotypeMolecular profileTherapeutic targetPhenotypeTarget therapeuticsBilateral cancerHistologic subclassificationDuctal carcinomaDifferent histologyProfilingHuman backgroundInvasive tumorsMucin productionCellular infiltrationMucinous phenotypeMolecular classification