2017
A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
Cullati SN, Kabeche L, Kettenbach AN, Gerber SA. A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase. Journal Of Cell Biology 2017, 216: 2339-2354. PMID: 28630147, PMCID: PMC5551695, DOI: 10.1083/jcb.201512055.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnaphaseChromatography, AffinityCytokinesisHeLa CellsHumansHydrolysisIntracellular Signaling Peptides and ProteinsKinesinsNIMA-Related KinasesOncogene ProteinsPhosphorylationProtein Serine-Threonine KinasesRNA InterferenceSignal TransductionSpindle ApparatusTandem Mass SpectrometryTime FactorsTransfectionConceptsProtein-protein interactionsAnaphase central spindleNEK kinasesKinase NEK6Distinct kinesinsBundling activityPosttranslational modificationsCentral spindleCytoskeletal structuresNek9MKlp2Genetic materialTimely localizationKinesinCytokinesisNEK6Sophisticated networkKinaseNIMANEK7KIF14CascadeAnaphaseLocalizationMidzone
2012
Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore–microtubule attachments
Maia AR, Garcia Z, Kabeche L, Barisic M, Maffini S, Macedo-Ribeiro S, Cheeseman IM, Compton DA, Kaverina I, Maiato H. Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore–microtubule attachments. Journal Of Cell Biology 2012, 199: 285-301. PMID: 23045552, PMCID: PMC3471233, DOI: 10.1083/jcb.201203091.Peer-Reviewed Original ResearchConceptsSpindle assembly checkpointKT-MT attachmentsChromosome alignmentAccurate chromosome segregationC-terminal phosphorylationKinetochore-microtubule attachmentsUnderlying regulatory mechanismsPlk1 recruitmentMitosis reliesChromosome segregationChromosome movementMT attachmentPlk1 kinaseSpindle bipolarityMicrotubule interfaceMT dynamicsRegulatory mechanismsCLASP2Plk1CDK1PhosphorylationAttachment stabilityKinetochoresMitosisKinase