2023
ATR promotes clearance of damaged DNA and damaged cells by rupturing micronuclei
Joo Y, Black E, Trier I, Haakma W, Zou L, Kabeche L. ATR promotes clearance of damaged DNA and damaged cells by rupturing micronuclei. Molecular Cell 2023, 83: 3642-3658.e4. PMID: 37788673, PMCID: PMC10599252, DOI: 10.1016/j.molcel.2023.09.003.Peer-Reviewed Original ResearchMeSH KeywordsAtaxia Telangiectasia Mutated ProteinsDNADNA DamageHumansLamin Type ANucleotidyltransferasesPhosphorylationConceptsATR inhibitorsLamin A/CCytoplasmic DNA sensor cGASHuman ataxia telangiectasiaDNA sensor cGASCdk1 phosphorylationGenomic integrityKinase functionMicronuclear DNAAtaxia telangiectasiaCDK1 inhibitionChromosomal instabilityS phaseAutonomous mechanismsAutophagosome accumulationATRCancer cellsDNACell populationsCGASCellsMicronucleiRad3GenomeChromothripsis
2017
A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase
Cullati SN, Kabeche L, Kettenbach AN, Gerber SA. A bifurcated signaling cascade of NIMA-related kinases controls distinct kinesins in anaphase. Journal Of Cell Biology 2017, 216: 2339-2354. PMID: 28630147, PMCID: PMC5551695, DOI: 10.1083/jcb.201512055.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnaphaseChromatography, AffinityCytokinesisHeLa CellsHumansHydrolysisIntracellular Signaling Peptides and ProteinsKinesinsNIMA-Related KinasesOncogene ProteinsPhosphorylationProtein Serine-Threonine KinasesRNA InterferenceSignal TransductionSpindle ApparatusTandem Mass SpectrometryTime FactorsTransfectionConceptsProtein-protein interactionsAnaphase central spindleNEK kinasesKinase NEK6Distinct kinesinsBundling activityPosttranslational modificationsCentral spindleCytoskeletal structuresNek9MKlp2Genetic materialTimely localizationKinesinCytokinesisNEK6Sophisticated networkKinaseNIMANEK7KIF14CascadeAnaphaseLocalizationMidzone
2012
Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore–microtubule attachments
Maia AR, Garcia Z, Kabeche L, Barisic M, Maffini S, Macedo-Ribeiro S, Cheeseman IM, Compton DA, Kaverina I, Maiato H. Cdk1 and Plk1 mediate a CLASP2 phospho-switch that stabilizes kinetochore–microtubule attachments. Journal Of Cell Biology 2012, 199: 285-301. PMID: 23045552, PMCID: PMC3471233, DOI: 10.1083/jcb.201203091.Peer-Reviewed Original ResearchConceptsSpindle assembly checkpointKT-MT attachmentsChromosome alignmentAccurate chromosome segregationC-terminal phosphorylationKinetochore-microtubule attachmentsUnderlying regulatory mechanismsPlk1 recruitmentMitosis reliesChromosome segregationChromosome movementMT attachmentPlk1 kinaseSpindle bipolarityMicrotubule interfaceMT dynamicsRegulatory mechanismsCLASP2Plk1CDK1PhosphorylationAttachment stabilityKinetochoresMitosisKinase