2023
The ion transporter Na+-K+-ATPase enables pathological B cell survival in the kidney microenvironment of lupus nephritis
Chernova I, Song W, Steach H, Hafez O, Al Souz J, Chen P, Chandra N, Cantley L, Veselits M, Clark M, Craft J. The ion transporter Na+-K+-ATPase enables pathological B cell survival in the kidney microenvironment of lupus nephritis. Science Advances 2023, 9: eadf8156. PMID: 36724234, PMCID: PMC9891690, DOI: 10.1126/sciadv.adf8156.Peer-Reviewed Original ResearchConceptsB cellsAutoimmune diseasesAmelioration of proteinuriaLupus nephritis biopsiesB cell infiltrationSodium-potassium adenosine triphosphataseB cell survivalPotassium adenosine triphosphataseLupus nephritisCell infiltrationKidney microenvironmentTissue injuryTherapeutic targetPharmacological inhibitionElevated sodium concentrationLupusHostile microenvironmentHigh expressionKidneySodium concentrationGenetic knockoutCell survivalDiseaseCellsAdenosine triphosphatase
2022
Authors' Reply: Most Arginase-1 Positive Cells Are Likely Injured S3 Proximal Tubular Cells Carrying Upregulated Phagocytotic Capacity rather than M2 Macrophages—Too Many To Be True
Shin N, Marlier A, Xu L, Doilicho N, Linberg D, Guo J, Cantley L. Authors' Reply: Most Arginase-1 Positive Cells Are Likely Injured S3 Proximal Tubular Cells Carrying Upregulated Phagocytotic Capacity rather than M2 Macrophages—Too Many To Be True. Journal Of The American Society Of Nephrology 2022, 33: 2124-2125. PMID: 36316094, PMCID: PMC9678031, DOI: 10.1681/asn.2022070836.Peer-Reviewed Original Research
2019
Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry
Singh N, Avigan ZM, Kliegel JA, Shuch BM, Montgomery RR, Moeckel GW, Cantley LG. Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry. JCI Insight 2019, 4: e129477. PMID: 31217358, PMCID: PMC6629112, DOI: 10.1172/jci.insight.129477.Peer-Reviewed Original ResearchConceptsCell typesIndividual cell typesCritical baseline dataRenal cell typesMass cytometryQuantitative atlasNormal human samplesHuman kidneyRelative abundanceDevelopment of therapiesHuman kidney diseaseKidney diseaseMetal-conjugated antibodiesQuantitative interrogationScarce samplesMachine-learning pipelineDiscovery purposesFuture quantitative analysisNovel abnormalityNormal human kidneySingle tissue sectionHuman samplesRenal biopsyImmune cellsCells
2013
The Terminator mouse is a diphtheria toxin–receptor knock-in mouse strain for rapid and efficient enrichment of desired cell lineages
Guo JK, Shi H, Koraishy F, Marlier A, Ding Z, Shan A, Cantley LG. The Terminator mouse is a diphtheria toxin–receptor knock-in mouse strain for rapid and efficient enrichment of desired cell lineages. Kidney International 2013, 84: 1041-1046. PMID: 23739236, PMCID: PMC3775868, DOI: 10.1038/ki.2013.202.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersBlotting, WesternCell LineageCell SeparationCell SurvivalDiphtheria ToxinGenotypeHeparin-binding EGF-like Growth FactorImmunohistochemistryIntegrasesIntercellular Signaling Peptides and ProteinsKidney Tubules, ProximalMiceMice, Inbred C57BLMice, TransgenicPhenotypePodocytesPrimary Cell CultureRNA, UntranslatedTime FactorsConceptsDiphtheria toxin treatmentToxin treatmentPrimary culturesDiphtheria toxin receptor expressionDiphtheria toxin receptorCell typesProximal tubule cellsPodocin-cre miceToxin exposureTubule cellsMiceMouse strainsWestern blottingToxin receptorKidney cellsSpecific cell typesMarker proteinsCell lineagesCellsTreatmentBrg1 Determines Urothelial Cell Fate during Ureter Development
Weiss RM, Guo S, Shan A, Shi H, Romano RA, Sinha S, Cantley LG, Guo JK. Brg1 Determines Urothelial Cell Fate during Ureter Development. Journal Of The American Society Of Nephrology 2013, 24: 618-626. PMID: 23449535, PMCID: PMC3609140, DOI: 10.1681/asn.2012090902.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsUreter developmentBasal cell populationUreteral smooth muscle cellsHoxb7-CreTerminal differentiationPPARγ expressionUreteral epitheliumMuscle cellsUrothelial cellsP63 expressionRole of BRG1Basal cellsUmbrella cellsCell populationsSonic hedgehog expressionEpithelial stratificationAdult ureterUreterCell developmentBRG1 expressionSmooth muscle cell developmentShh expressionCellsHedgehog expression
2009
Chapter 23 An Overview of Renal Development
Marlier A, Cantley L. Chapter 23 An Overview of Renal Development. 2009, 365-392. DOI: 10.1016/b978-0-12-449851-8.00023-1.Peer-Reviewed Original ResearchMetanephric mesenchymeNephric ductUreteric budCell deathBcl-2Anti-apoptotic proteinsRegulated processDifferentiation signalsUB tipsSix2 expressionUninduced mesenchymeMetanephric kidneyRenal vesiclesMesenchymeRenal developmentBudsNearby cellsUbProteinExpressionApoptosisLow expressionCellsOutgrowthInitial outgrowth
2001
HGF promotes adhesion of ATP-depleted renal tubular epithelial cells in a MAPK-dependent manner
Liu Z, Nickel C, Cantley L. HGF promotes adhesion of ATP-depleted renal tubular epithelial cells in a MAPK-dependent manner. American Journal Of Physiology. Renal Physiology 2001, 281: f62-f70. PMID: 11399647, DOI: 10.1152/ajprenal.2001.281.1.f62.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseATP-depleted cellsHepatocyte growth factorCell adhesionAddition of HGFATP depletionExtracellular signal-regulated kinase (ERK) phosphorylationMAPK-dependent mannerSignal-regulated kinase phosphorylationLoss of adhesionDuct 3 cellsEffects of HGFProtein kinaseERK activationMAPK pathwayKinase phosphorylationRenal tubular epithelial cellsEpithelial cellsAdherent cellsGrowth factorCellsATPTubular epithelial cellsPathwayAdhesionCell Surface Glypicans Are Low-Affinity Endostatin Receptors
Karumanchi S, Jha V, Ramchandran R, Karihaloo A, Tsiokas L, Chan B, Dhanabal M, Hanai J, Venkataraman G, Shriver Z, Keiser N, Kalluri R, Zeng H, Mukhopadhyay D, Chen R, Lander A, Hagihara K, Yamaguchi Y, Sasisekharan R, Cantley L, Sukhatme V. Cell Surface Glypicans Are Low-Affinity Endostatin Receptors. Molecular Cell 2001, 7: 811-822. PMID: 11336704, DOI: 10.1016/s1097-2765(01)00225-8.Peer-Reviewed Original ResearchConceptsHeparan sulfate glycosaminoglycansPotent anti-angiogenic proteinCell surface proteoglycansBranching morphogenesisEndothelial cellsGenetic studiesGlypicansExpression cloningSurface proteoglycansAntisense experimentsCell surfaceAnti-angiogenic proteinEndothelial cell surfaceLow-affinity receptorsSulfate glycosaminoglycansEndostatin bindingCellsReceptorsMorphogenesisEndostatin activityCloningProteinEndostatinBindingProteoglycans
1999
Activated ERK2 Interacts with and Phosphorylates the Docking Protein GAB1*
Roshan B, Kjelsberg C, Spokes K, Eldred A, Crovello C, Cantley L. Activated ERK2 Interacts with and Phosphorylates the Docking Protein GAB1*. Journal Of Biological Chemistry 1999, 274: 36362-36368. PMID: 10593929, DOI: 10.1074/jbc.274.51.36362.Peer-Reviewed Original Research
1998
Activation of Phospholipase C-γ by Phosphatidylinositol 3,4,5-Trisphosphate*
Bae Y, Cantley L, Chen C, Kim S, Kwon K, Rhee S. Activation of Phospholipase C-γ by Phosphatidylinositol 3,4,5-Trisphosphate*. Journal Of Biological Chemistry 1998, 273: 4465-4469. PMID: 9468499, DOI: 10.1074/jbc.273.8.4465.Peer-Reviewed Original ResearchConceptsPLC-gamma isozymesPhospholipase CSrc homology 2 domainNIH 3T3 cellsCOS-7 cellsSpecific phospholipase CPLC gamma tyrosine phosphorylationAction of PISignal transductionCatalytic subunitTyrosine phosphorylationSpecific PIInhibitor LY294002PLC activationCell membranePhosphatidylinositolInositol phosphate formationIsozymesActivationCellsTransductionPIPhosphorylationSubunitsBisphosphate
1997
The Lipid Products of Phosphoinositide 3-Kinase Increase Cell Motility through Protein Kinase C*
Derman M, Toker A, Hartwig J, Spokes K, Falck JR, Chen C, Cantley L, Cantley L. The Lipid Products of Phosphoinositide 3-Kinase Increase Cell Motility through Protein Kinase C*. Journal Of Biological Chemistry 1997, 272: 6465-6470. PMID: 9045671, DOI: 10.1074/jbc.272.10.6465.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAndrostadienesAnimalsCell MembraneCell MovementDown-RegulationEnzyme ActivationEnzyme InhibitorsMiceNaphthalenesPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphotransferases (Alcohol Group Acceptor)Platelet-Derived Growth FactorProtein Kinase CSignal TransductionWortmanninConceptsProtein kinase CCell motilityLipid productsKinase CPKC family membersNIH 3T3 cellsIndependent PKC isoformsMembrane rufflingActin reorganizationCellular motilityPKC isoformsPtdInsVivo rolePhosphoinositideMotilityFirst demonstrationRecent studiesFamily membersComplete inhibitionCellsRufflingPhosphatidylinositolPrevious observationsIsoformsTrisphosphate