1997
Modulation of c-fos and egr-1 expression in the isolated perfused kidney by agents that alter tubular work
Joannidis M, Cantley L, Spokes K, Stuart-Tilley A, Alper S, Epstein F. Modulation of c-fos and egr-1 expression in the isolated perfused kidney by agents that alter tubular work. Kidney International 1997, 52: 130-139. PMID: 9211355, DOI: 10.1038/ki.1997.312.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsAnimalsBlotting, NorthernCell HypoxiaCells, CulturedDNA-Binding ProteinsDogsEarly Growth Response Protein 1Fluorescent Antibody Technique, IndirectGene Expression RegulationGlycineImmediate-Early ProteinsImmunohistochemistryIn Vitro TechniquesKidneyMaleOuabainProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleyTime FactorsTranscription FactorsConceptsMedullary thick ascending limbThick ascending limbHypoxic injuryOuter medullaStandard perfusionC-fosMRNA levelsIEG expressionAscending limbEgr-1Immediate early gene c-fosAbsence of reperfusionEarly gene c-fosKrebs-Henseleit bufferGene c-fosImmediate early gene expressionInhibition of NaEgr-1 expressionHypoxic damageRenal cortexImmunohistochemical demonstrationRenal epithelial cellsTubular transportCultured renal epithelial cellsIEG mRNA levels
1995
Induction of heat-shock proteins does not prevent renal tubular injury following ischemia
Joannidis M, Cantley L, Spokes K, Medina R, Pullman J, Rosen S, Epstein F. Induction of heat-shock proteins does not prevent renal tubular injury following ischemia. Kidney International 1995, 47: 1752-1759. PMID: 7643546, DOI: 10.1038/ki.1995.242.Peer-Reviewed Original ResearchConceptsHeat shock proteinsMedullary hypoxic injuryPossible protective effectRenal tubular injuryDifferent experimental modelsSevere morphological damageSerum creatinineTubular injuryCross clampingIschemic injuryRight kidneyRenal arteryHypoxic injuryControl ratsIntact ratsRenal cortexProtective effectImmunohistochemical meansUrea nitrogenProximal tubulesHeat shockRenal cellsRat kidneyKidneyMorphological damage