2002
A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex
Hudson AM, Cooley L. A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex. Journal Of Cell Biology 2002, 156: 677-687. PMID: 11854308, PMCID: PMC2174088, DOI: 10.1083/jcb.200109065.Peer-Reviewed Original ResearchConceptsArp2/3 complexRing canal growthActin-related proteinsParallel actin bundlesNurse cell cytoplasmActin filament nucleationDynamic actin rearrangementsActin cytoskeletonRing canalsActin structuresSlow spontaneous rateActin rearrangementPupal epitheliumPlasma membraneFilament nucleationShaft cellsActin bundlesActin filamentsComplex contributesFunction mutationsCanal growthCell cytoplasmSubunitsMutationsComplexes
1999
Drosophila quail, a villin-related protein, bundles actin filaments in apoptotic nurse cells
Matova N, Mahajan-Miklos S, Mooseker M, Cooley L. Drosophila quail, a villin-related protein, bundles actin filaments in apoptotic nurse cells. Development 1999, 126: 5645-5657. PMID: 10572041, DOI: 10.1242/dev.126.24.5645.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActinsAmino Acid SequenceAnimalsApoptosisBiological TransportCalciumCarrier ProteinsCloning, MolecularCytoplasmDrosophila melanogasterEscherichia coliHumansInsect ProteinsMicrofilament ProteinsMolecular Sequence DataRecombinant Fusion ProteinsSequence Homology, Amino AcidConceptsEgg chambersNurse cellsFilamentous actinActin filamentsCytoplasm transportNuclear envelopeQuail proteinGermline-specific proteinsMutant egg chambersNurse cell apoptosisActin bundle assemblyNew actin filamentsApoptotic nurse cellsActin-regulating proteinsBundles actin filamentsHuman villinDrosophila germlineSequence homologyBiochemical experimentsActin bundlesElevated cytoplasmic calciumProteinVillinActinAbundant networkDrosophila Filamin encoded by the cheerio locus is a component of ovarian ring canals
Sokol N, Cooley L. Drosophila Filamin encoded by the cheerio locus is a component of ovarian ring canals. Current Biology 1999, 9: 1221-1230. PMID: 10556087, DOI: 10.1016/s0960-9822(99)80502-8.Peer-Reviewed Original ResearchConceptsRing canalsDrosophila filaminPlasma membraneFilamentous actinActin filamentsOvarian ring canalsFruit fly DrosophilaLoss of filaminActin-binding domainActin-binding proteinsParallel actin bundlesLocalization of filaminFlow of cytoplasmFly DrosophilaActin structuresFilamin isoformsTransmembrane proteinCell cortexActin arraysContractile ringKelch geneCytoskeletal proteinsCleavage furrowStress fibersActin bundles
1998
Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail
Cant K, Knowles B, Mahajan-Miklos S, Heintzelman M, Cooley L. Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail. Journal Of Cell Science 1998, 111: 213-221. PMID: 9405306, DOI: 10.1242/jcs.111.2.213.Peer-Reviewed Original ResearchConceptsActin-bundling proteinActin bundle formationVillin-like proteinBundling proteinNurse cellsActin bundle assemblyCytoplasmic actin bundlesDistinct biochemical propertiesBundle formationCytoplasmic actin networksQuail geneDrosophila germlineDrosophila oogenesisFascin functionGermline transformationEgg chambersCytoplasm transportDrosophila bristlesRedundant functionsActin networkActin bundlesWild typeBundle assemblyQuail proteinSpecialized structures
1996
Single Amino Acid Mutations in Drosophila Fascin Disrupt Actin Bundling Function in Vivo
Cant K, Cooley L. Single Amino Acid Mutations in Drosophila Fascin Disrupt Actin Bundling Function in Vivo. Genetics 1996, 143: 249-258. PMID: 8722779, PMCID: PMC1207258, DOI: 10.1093/genetics/143.1.249.Peer-Reviewed Original ResearchConceptsEMS mutagenesis screenMutagenesis screenCytoplasm transportActin-bundling functionDiverse cellular processesIntragenic suppressor mutationsBundles actin filamentsCytoplasmic actin bundlesSingle amino acid mutationSerine 289Glutamic acid resultsAmino acid mutationsDominant suppressorsFascin functionFemale sterileSuppressor mutationsCellular processesC-terminusActin bundlesCentral domainActin filamentsSevere defectsMicrovillar projectionsAcid mutationsFilopodial extensions
1994
Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension.
Cant K, Knowles BA, Mooseker MS, Cooley L. Drosophila singed, a fascin homolog, is required for actin bundle formation during oogenesis and bristle extension. Journal Of Cell Biology 1994, 125: 369-380. PMID: 8163553, PMCID: PMC2120035, DOI: 10.1083/jcb.125.2.369.Peer-Reviewed Original ResearchConceptsActin filament bundle formationActin filament bundlesSevere mutantsBundle formationFilament bundlesActin bundle formationBundles actin filamentsNurse cell nucleiDrosophila homologBristle phenotypeSocket cellsFemale sterileEgg chambersRing canalsCytoplasm transportSea urchin eggsNurse cellsActin bundlesCellular extensionsSevere allelesActin filamentsDrosophilaMutantsMigratory cellsFilopodial extensions