2005
Drosophila myosin V is required for larval development and spermatid individualization
Mermall V, Bonafé N, Jones L, Sellers JR, Cooley L, Mooseker MS. Drosophila myosin V is required for larval development and spermatid individualization. Developmental Biology 2005, 286: 238-255. PMID: 16126191, DOI: 10.1016/j.ydbio.2005.07.028.Peer-Reviewed Original ResearchConceptsInvestment conesLarval developmentClass V myosinsIndividualization complexSpermatid individualizationCytological defectsTruncation alleleVesicular trafficRNA transportActin structuresLarval tissuesMutant animalsMature spermSperm nucleiMyoVSpermatid maturationMolecular motorsMyosin VMechanochemical couplingDetectable defectsV geneMicrotubulesIndividual membranesActinSpermatogenesis
2002
Arp2/3-Dependent Psuedocleavage Furrow Assembly in Syncytial Drosophila Embryos
Stevenson V, Hudson A, Cooley L, Theurkauf WE. Arp2/3-Dependent Psuedocleavage Furrow Assembly in Syncytial Drosophila Embryos. Current Biology 2002, 12: 705-711. PMID: 12007413, DOI: 10.1016/s0960-9822(02)00807-2.Peer-Reviewed Original ResearchConceptsDrosophila embryosPseudocleavage furrowsCell cycleActin capActin reorganizationSomatic cell divisionLocal actin polymerizationSyncytial Drosophila embryosARPC1 subunitArp2/3 complexNuclear positioningEmbryonic divisionsComplex localizeCell divisionActin polymerizationCleavage furrowFurrow formationCap functionSpindle fusionMolecular mechanismsArp2/3EmbryosVivo analysisMutationsAssembly
1999
Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila
Buszczak M, Freeman M, Carlson J, Bender M, Cooley L, Segraves W. Ecdysone response genes govern egg chamber development during mid-oogenesis in Drosophila. Development 1999, 126: 4581-4589. PMID: 10498692, DOI: 10.1242/dev.126.20.4581.Peer-Reviewed Original ResearchConceptsEgg chamber developmentGermline clonesResponse genesGene expressionDorsal follicle cell fatesEgg chamber maturationMutant egg chambersFollicle cell fatesSteroid hormone ecdysoneEcdysone response genesProgression of oogenesisTemperature-sensitive mutationStage-specific mannerTemporal expression profilesEarly response genesChamber developmentEcdysone responsivenessChamber maturationHormone ecdysoneGenetic hierarchyEcR expressionDrosophila melanogasterEgg chambersCell fateEarly gene expression
1998
Apoptosis in late stage Drosophila nurse cells does not require genes within the H99 deficiency
Foley K, Cooley L. Apoptosis in late stage Drosophila nurse cells does not require genes within the H99 deficiency. Development 1998, 125: 1075-1082. PMID: 9463354, DOI: 10.1242/dev.125.6.1075.Peer-Reviewed Original ResearchConceptsEgg chambersNurse cellsDNA fragmentationDrosophila nurse cellsMutant egg chambersDrosophila egg chamberOvarian expression patternsDrosophila apoptosisGermline clonesHead involutionCytoplasm transportPositive regulatorRegulatory genesStage 13Cytoplasm transferApoptotic vesiclesCytoplasmic factorsNegative regulatorExpression patternsWild typeGenesFragmented DNAOogenesisApoptosisStage 12Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail
Cant K, Knowles B, Mahajan-Miklos S, Heintzelman M, Cooley L. Drosophila fascin mutants are rescued by overexpression of the villin-like protein, quail. Journal Of Cell Science 1998, 111: 213-221. PMID: 9405306, DOI: 10.1242/jcs.111.2.213.Peer-Reviewed Original ResearchConceptsActin-bundling proteinActin bundle formationVillin-like proteinBundling proteinNurse cellsActin bundle assemblyCytoplasmic actin bundlesDistinct biochemical propertiesBundle formationCytoplasmic actin networksQuail geneDrosophila germlineDrosophila oogenesisFascin functionGermline transformationEgg chambersCytoplasm transportDrosophila bristlesRedundant functionsActin networkActin bundlesWild typeBundle assemblyQuail proteinSpecialized structures
1997
Examination of the function of two kelch proteins generated by stop codon suppression
Robinson D, Cooley L. Examination of the function of two kelch proteins generated by stop codon suppression. Development 1997, 124: 1405-1417. PMID: 9118811, DOI: 10.1242/dev.124.7.1405.Peer-Reviewed Original ResearchMeSH KeywordsAlanineAnimalsAnimals, Genetically ModifiedCarrier ProteinsCodon, TerminatorDrosophilaDrosophila ProteinsFemaleGene Expression Regulation, DevelopmentalImmunohistochemistryInfertility, FemaleInsect ProteinsMaleMicrofilament ProteinsMutationOogenesisOpen Reading FramesOvaryRNA, MessengerSuppression, GeneticTissue DistributionConceptsRing canalsKelch proteinStop codon suppressionStop codonCodon suppressionDrosophila kelch geneOvarian ring canalsUGA stop codonFull-length proteinOpen reading frameTissue-specific mannerUAA stop codonFemale sterilitySense codonsReading frameSingle transcriptKelch geneORF1 proteinCodonKelchDifferent tissuesProteinMutantsORF1Transcripts
1994
Morphogenesis of Drosophila ovarian ring canals
Robinson D, Cant K, Cooley L. Morphogenesis of Drosophila ovarian ring canals. Development 1994, 120: 2015-2025. PMID: 7925006, DOI: 10.1242/dev.120.7.2015.Peer-Reviewed Original ResearchConceptsRing canalsEgg chambersHu-li tai shaoMutant egg chambersOvarian ring canalsEgg chamber developmentDrosophila egg chamberCanal developmentCarboxy-terminal tailCytoskeletal protein assemblyAnti-phosphotyrosine antibodyProtein assembliesHT proteinsTyrosine phosphorylationFilamentous actinCytoplasmic bridgesActinChamber developmentKelchProteinCanal componentsAssemblyMorphogenesisGermariumPhosphorylation
1984
The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation.
Schaack J, Sharp S, Dingermann T, Burke DJ, Cooley L, Söll D. The extent of a eukaryotic tRNA gene. 5‘- and 3‘-flanking sequence dependence for transcription and stable complex formation. Journal Of Biological Chemistry 1984, 259: 1461-1467. PMID: 6693417, DOI: 10.1016/s0021-9258(17)43429-6.Peer-Reviewed Original ResearchConceptsStable complex formationBase pairsDrosophila Kc cell extractSequence requirementsCell extractsEukaryotic tRNA genesStable transcription complexesHeLa cell extractsTRNA genesComplex formationTranscription complexArg genesEfficient transcriptionTranscription assaysTranscription propertiesCell-free extractsTranscriptionHomologous systemGenesSequenceSequence dependenceCellular sourceExtractAssaysPairs