A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics
Champion EA, Kundrat L, Regan L, Baserga SJ. A structural model for the HAT domain of Utp6 incorporating bioinformatics and genetics. Protein Engineering Design And Selection 2009, 22: 431-439. PMID: 19515729, PMCID: PMC2699269, DOI: 10.1093/protein/gzp022.Peer-Reviewed Original ResearchConceptsHAT domainRepeat motifsTetratricopeptide repeat (TPR) motifsKey functional rolesStructure-function relationshipsRNA metabolismLoss of functionMutagenesis studiesInteraction surfaceHomology modelingTertiary structureHomology modelFunctional roleMotifResiduesUTP6BioinformaticsProteinStructural contextDirect effectDomainMutagenesisGeneticsFunction mapsMutationsRedesign of a protein–peptide interaction: Characterization and applications
Jackrel ME, Valverde R, Regan L. Redesign of a protein–peptide interaction: Characterization and applications. Protein Science 2009, 18: 762-774. PMID: 19309728, PMCID: PMC2762588, DOI: 10.1002/pro.75.Peer-Reviewed Original ResearchConceptsProtein-peptide interactionsProtein-peptide pairsPurification applicationsRecognition pairsCorresponding peptide ligandsRepeat protein scaffoldsMolecular recognitionAntibody-antigen interactionsFacile replacementChemical considerationsProtein scaffoldsPeptide ligandsTypical antibody-antigen interactionsHarsh conditionsProtein designModerate affinityScaffoldsPractical applicationsAffinity purificationAffinityLigandsPropertiesInteractionSuccessful applicationDissociation