2014
Methylation of Twelve CpGs in Human Papillomavirus Type 16 (HPV16) as an Informative Biomarker for the Triage of Women Positive for HPV16 Infection
Brandsma JL, Harigopal M, Kiviat NB, Sun Y, Deng Y, Zelterman D, Lizardi PM, Shabanova VS, Levi A, Yaping T, Hu X, Feng Q. Methylation of Twelve CpGs in Human Papillomavirus Type 16 (HPV16) as an Informative Biomarker for the Triage of Women Positive for HPV16 Infection. Cancer Prevention Research 2014, 7: 526-533. PMID: 24556390, DOI: 10.1158/1940-6207.capr-13-0354.Peer-Reviewed Original ResearchConceptsCervical intraepithelial neoplasiaCytology samplesCutoff scoreColposcopy of womenCervical cancer preventionManagement of womenDetection of CIN3Cervical cytology samplesHuman papillomavirus type 16Human papillomavirus type 16 DNAMethylation biomarkersPapillomavirus type 16Histologic severityIntraepithelial neoplasiaCervical cancerHPV16 infectionCancer preventionDNA bisulfite sequencingType 16Prognostic potentialAccurate biomarkersProspective biomarkersScreening testInformative biomarkersBiomarkers
2012
Increasing Cytotechnologist Workload Above 100 Slides per Day Using the BD FocalPoint GS Imaging System Negatively Affects Screening Performance
Levi AW, Galullo P, Gordy K, Mikolaiski N, Schofield K, Elsheikh TM, Harigopal M, Chhieng DC. Increasing Cytotechnologist Workload Above 100 Slides per Day Using the BD FocalPoint GS Imaging System Negatively Affects Screening Performance. American Journal Of Clinical Pathology 2012, 138: 811-815. PMID: 23161714, DOI: 10.1309/ajcptydd9g2ncumf.Peer-Reviewed Original ResearchMeSH KeywordsCarcinoma, Squamous CellCytodiagnosisDiagnostic ImagingFalse Negative ReactionsFemaleHumansImage Processing, Computer-AssistedMass ScreeningMedical Laboratory PersonnelQuality ControlSensitivity and SpecificityTime FactorsUterine Cervical DysplasiaUterine Cervical NeoplasmsVaginal SmearsWorkloadThe Positive Impact of Simultaneous Implementation of the BD FocalPoint GS Imaging System and Lean Principles on the Operation of Gynecologic Cytology
Wong R, Levi AW, Harigopal M, Schofield K, Chhieng DC. The Positive Impact of Simultaneous Implementation of the BD FocalPoint GS Imaging System and Lean Principles on the Operation of Gynecologic Cytology. Archives Of Pathology & Laboratory Medicine 2012, 136: 183-9. PMID: 22288966, DOI: 10.5858/arpa.2011-0139-oa.Peer-Reviewed Original Research
2011
Implementation of FocalPoint GS location‐guided imaging system
Levi AW, Chhieng DC, Schofield K, Kowalski D, Harigopal M. Implementation of FocalPoint GS location‐guided imaging system. Cancer Cytopathology 2011, 120: 126-133. PMID: 22505211, DOI: 10.1002/cncy.20187.Peer-Reviewed Original ResearchConceptsASC-US casesHR-HPV positive rateLow-risk patient populationHigh-risk human papillomavirusASC-US interpretationsRoutine clinical settingPromising screening toolPap testHuman papillomavirusPapanicolaou testPatient populationBacterial vaginosisDrug AdministrationSIL ratioClinical settingScreening toolDiagnostic categoriesLaboratory information systemGynecologic cytologyPrimary screeningSignificant increaseFalse negative fractionPositive rateHigh percentageCurrent studyUse of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions
Levi AW, Harigopal M, Hui P, Schofield K, Chhieng DC. Use of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions. Cancer Cytopathology 2011, 119: 228-234. PMID: 21732551, DOI: 10.1002/cncy.20172.Peer-Reviewed Original ResearchConceptsLow-grade squamous intraepithelial lesionsHR-HPV testingHPV testingCIN 2Colposcopy rateHR-HPVRelative light units/cutoffCervical intraepithelial neoplasia 2High-risk human papillomavirusHR-HPV statusRLU/COIntraepithelial neoplasia 2Hybrid Capture 2Squamous intraepithelial lesionsCIN 1 lesionsLarge teaching hospitalNegative predictive valueLSIL cytologyHPV statusIntraepithelial lesionsCapture 2Human papillomavirusTeaching hospitalCytologic diagnosisSevere lesions
2009
Distinct human papillomavirus type 16 methylomes in cervical cells at different stages of premalignancy
Brandsma JL, Sun Y, Lizardi PM, Tuck DP, Zelterman D, Haines GK, Martel M, Harigopal M, Schofield K, Neapolitano M. Distinct human papillomavirus type 16 methylomes in cervical cells at different stages of premalignancy. Virology 2009, 389: 100-107. PMID: 19443004, PMCID: PMC2918277, DOI: 10.1016/j.virol.2009.03.029.Peer-Reviewed Original ResearchConceptsRoutine cervical cancer screeningCervical cellsCervical cancer screeningHigh-grade lesionsLow-grade lesionsHPV16-positive samplesCervical progressionNegative cytologyCervical lesionsCancer screeningCervical samplesPathologic diagnosisCervical carcinogenesisClinical significanceHuman papillomavirus gene expressionLow-level methylationLarger studyE6 geneL1 regionLesionsAbnormal patternsClinical samplesPattern ADistinct methylation patternsHigh-level methylation
2004
Comparison of human papillomavirus DNA prevalence in atypical squamous cells of undetermined significance subcategories as defined by the original Bethesda 1991 and the new Bethesda 2001 Systems.
Pirog EC, Erroll M, Harigopal M, Centeno BA. Comparison of human papillomavirus DNA prevalence in atypical squamous cells of undetermined significance subcategories as defined by the original Bethesda 1991 and the new Bethesda 2001 Systems. Archives Of Pathology & Laboratory Medicine 2004, 128: 527-32. PMID: 15086283, DOI: 10.5858/2004-128-527-cohpdp.Peer-Reviewed Original ResearchConceptsAtypical squamous cellsHigh-grade squamous intraepithelial lesionsTBS 2001Squamous intraepithelial lesionsSquamous cellsASCUS casesIntraepithelial lesionsIndividual morphologic featuresMorphologic featuresHPV DNAHPV detectionPap testAtypical cellsUndetermined significanceBroad-spectrum HPV DNA amplificationControl groupHuman papillomavirus (HPV) DNA prevalenceASC-H casesBethesda 2001 systemPrevalence of HPVThinPrep Pap testsHPV DNA amplificationHuman papillomavirus DNAPanel of pathologistsBethesda System 2001