1998
Purinergic regulation of acid/base transport in human and rat biliary epithelial cell lines
Zsembery Á, Spirlì C, Granato A, LaRusso N, Okolicsanyi L, Crepaldi G, Strazzabosco M. Purinergic regulation of acid/base transport in human and rat biliary epithelial cell lines. Hepatology 1998, 28: 914-920. PMID: 9755225, DOI: 10.1002/hep.510280403.Peer-Reviewed Original ResearchConceptsCholangiocyte cell lineNHE activityPurinergic agonistsBiliary epithelial cell lineExtracellular ATPCell linesIntracellular cyclic adenosine monophosphate (cAMP) concentrationsBiliary HCO3- secretionRat cholangiocyte cell lineCyclic adenosine monophosphate concentrationsAdenosine receptor agonistsBiliary epithelial cellsMz-ChA-1 cellsAdenosine monophosphate concentrationsSynergistic stimulatory effectApical administrationEpithelial cell lineReceptor agonistP2Y2 receptorPurinergic receptorsBile volumePharmacological profileApical Cl- channelsBasolateral administrationHCO3- secretion
1995
Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor‐α, and insulinlike growth factor‐II on Na+/H+ exchange activity
Strazzabosco M, Poci C, Spirlì C, Zsembery A, Granato A, Massimino M, Crepaldi G. Intracellular pH regulation in hep G2 cells: Effects of epidermal growth factor, transforming growth factor‐α, and insulinlike growth factor‐II on Na+/H+ exchange activity. Hepatology 1995, 22: 588-597. PMID: 7635429, DOI: 10.1002/hep.1840220232.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAmilorideAmmonium ChlorideAnimalsCarcinoma, HepatocellularCarrier ProteinsCattleCell DivisionDNAEpidermal Growth FactorFetal BloodFluoresceinsFluorescent DyesHumansHydrogen-Ion ConcentrationInsulin-Like Growth Factor IILiver NeoplasmsSodium-Bicarbonate SymportersSodium-Hydrogen ExchangersTransforming Growth Factor alphaTumor Cells, CulturedConceptsInsulinlike growth factor IIEpidermal growth factorTGF-alphaGrowth factor IIHep G2 cellsGrowth factorFactor IIG2 cellsAdministration of EGFLiver growth factorCl/HCO3 exchangeExtrahepatic tumorsAcid/base homeostasisHepatic tumorsAcid loaderFetal calf serumAcid extrudersHepatoma cell linePresence of FCSAcid loadExchange activityMetabolic activationHCO3- exchangeProliferative stimulusAmiloride