RNA sequencing atopic dermatitis transcriptome profiling provides insights into novel disease mechanisms with potential therapeutic implications
Suárez-Fariñas M, Ungar B, da Rosa J, Ewald DA, Rozenblit M, Gonzalez J, Xu H, Zheng X, Peng X, Estrada YD, Dillon SR, Krueger JG, Guttman-Yassky E. RNA sequencing atopic dermatitis transcriptome profiling provides insights into novel disease mechanisms with potential therapeutic implications. Journal Of Allergy And Clinical Immunology 2015, 135: 1218-1227. PMID: 25840722, DOI: 10.1016/j.jaci.2015.03.003.Peer-Reviewed Original ResearchMeSH KeywordsAdultComputational BiologyDermatitis, AtopicFemaleGene Expression ProfilingHumansInterleukin-1MaleMembrane GlycoproteinsMiddle AgedMolecular Sequence AnnotationReceptors, ImmunologicReproducibility of ResultsSequence Analysis, RNASignal TransductionSkinTranscriptomeTriggering Receptor Expressed on Myeloid Cells-1ConceptsAtopic dermatitisAD transcriptomeNonlesional skinMyeloid cells 1 (TREM1) signalingUnderstanding of ADTREM-1 pathwayRT-PCRSevere atopic dermatitisIL-36 cytokinesInfection-related inflammationNovel therapeutic targetPotential therapeutic implicationsAD-related genesNovel disease mechanismsTREM-1Real-time PCRAdaptive immunityAD phenotypeTherapeutic implicationsTherapeutic targetNext-generation RNA sequencingDisease pathologyGenomic profilingSame cohortDisease transcriptomeIdentification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection
Esaki H, Ewald DA, Ungar B, Rozenblit M, Zheng X, Xu H, Estrada YD, Peng X, Mitsui H, Litman T, Suárez-Fariñas M, Krueger JG, Guttman-Yassky E. Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection. Journal Of Allergy And Clinical Immunology 2015, 135: 153-163. PMID: 25567045, PMCID: PMC4452382, DOI: 10.1016/j.jaci.2014.10.037.Peer-Reviewed Original ResearchConceptsNonlesional AD skinLaser capture microdissectionAD transcriptomeNormal skinAD skinNonlesional skinNovel ImmuneCapture microdissectionAtopic dermatitis lesionsBarrier genesPossible cellular sourcesAtopic dermatitisHealthy volunteersEpidermal alterationsBarrier phenotypeCellular sourceImmune moleculesCellular subsetsDermatitis lesionsImmuneDermal compartmentSkinGenomic profilesPatientsMolecular signatures