2014
Discovery and characterization of small molecules that target the GTPase Ral
Yan C, Liu D, Li L, Wempe MF, Guin S, Khanna M, Meier J, Hoffman B, Owens C, Wysoczynski CL, Nitz MD, Knabe WE, Ahmed M, Brautigan DL, Paschal BM, Schwartz MA, Jones DN, Ross D, Meroueh SO, Theodorescu D. Discovery and characterization of small molecules that target the GTPase Ral. Nature 2014, 515: 443-447. PMID: 25219851, PMCID: PMC4351747, DOI: 10.1038/nature13713.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP-Binding Cassette TransportersCell Line, TumorCell ProliferationComputer SimulationDrug Screening Assays, AntitumorFemaleGTPase-Activating ProteinsHumansMiceModels, MolecularMolecular Targeted TherapyNeoplasmsProtein BindingRal GTP-Binding ProteinsRas ProteinsSignal TransductionSmall Molecule LibrariesSubstrate SpecificityXenograft Model Antitumor Assays
2003
Differential αv integrin–mediated Ras-ERK signaling during two pathways of angiogenesis
Hood JD, Frausto R, Kiosses WB, Schwartz MA, Cheresh DA. Differential αv integrin–mediated Ras-ERK signaling during two pathways of angiogenesis. Journal Of Cell Biology 2003, 162: 933-943. PMID: 12952943, PMCID: PMC2172815, DOI: 10.1083/jcb.200304105.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChick EmbryoEnzyme ActivationFibroblast Growth Factor 2Focal Adhesion Protein-Tyrosine KinasesIntegrin alphaVbeta3IntegrinsMitogen-Activated Protein KinasesNeovascularization, PhysiologicP21-Activated KinasesProtein Serine-Threonine KinasesProtein-Tyrosine KinasesProto-Oncogene Proteins c-rafRas ProteinsReceptors, VitronectinSignal TransductionSrc-Family KinasesVascular Endothelial Growth Factor AConceptsPathways of angiogenesisC-RafDistinct vascular responsesDownstream of RasUpstream of RasC-Raf activationC-Raf activityKinase-dependent phosphorylationRas-ERK pathwayInhibition of FAKVascular responsesPhosphorylation/activationSignal-related kinaseEndothelial cell survivalSerine 338Blood vesselsRas-ERKActive RAChick chorioallantoic membraneRA activityAngiogenesisERK activityRetroviral deliveryVEGFCell survival
2000
Death Effector Domain Protein PEA-15 Potentiates Ras Activation of Extracellular Signal Receptor-activated Kinase by an Adhesion-independent Mechanism
Ramos J, Hughes P, Renshaw M, Schwartz M, Formstecher E, Chneiweiss H, Ginsberg M. Death Effector Domain Protein PEA-15 Potentiates Ras Activation of Extracellular Signal Receptor-activated Kinase by an Adhesion-independent Mechanism. Molecular Biology Of The Cell 2000, 11: 2863-2872. PMID: 10982386, PMCID: PMC14961, DOI: 10.1091/mbc.11.9.2863.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsApoptosis Regulatory ProteinsCell AdhesionCell LineCHO CellsCricetinaeEnzyme ActivationGuanosine TriphosphateHumansIntracellular Signaling Peptides and ProteinsJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase Kinase 1MiceMitogen-Activated Protein KinasesP38 Mitogen-Activated Protein KinasesPhosphoproteinsProtein Serine-Threonine KinasesRas ProteinsRecombinant Fusion ProteinsSignal TransductionConceptsPEA-15 expressionPEA-15ERK activationMitogen-activated protein kinase kinaseMitogen-activated protein kinase pathwayAdhesion-independent mechanismsRas-dependent mannerProtein kinase kinaseRegulation of apoptosisProtein kinase pathwayChinese hamster ovary cellsRas guanosineKinase kinaseRas activationSignal receptorHamster ovary cellsH-RasKinase pathwayERK activityIntegrin activationERK signalingAnchorage dependenceOncogenic processesOvary cellsApoptosis
1999
Focal Adhesion Kinase Mediates the Integrin Signaling Requirement for Growth Factor Activation of Map Kinase
Renshaw M, Price L, Schwartz M. Focal Adhesion Kinase Mediates the Integrin Signaling Requirement for Growth Factor Activation of Map Kinase. Journal Of Cell Biology 1999, 147: 611-618. PMID: 10545504, PMCID: PMC2151196, DOI: 10.1083/jcb.147.3.611.Peer-Reviewed Original Research3T3 CellsAnimalsCell AdhesionCell Adhesion MoleculesCell DivisionCell Line, TransformedCell Transformation, NeoplasticEnzyme ActivationFibroblastsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesGene DeletionGenes, DominantGrowth SubstancesIntegrin beta1MiceMitogen-Activated Protein Kinase 1Oncogene Protein pp60(v-src)Protein-Tyrosine KinasesRas ProteinsRecombinant Fusion ProteinsSignal TransductionTransfection
1997
Suppression of Integrin Activation: A Novel Function of a Ras/Raf-Initiated MAP Kinase Pathway
Hughes P, Renshaw M, Pfaff M, Forsyth J, Keivens V, Schwartz M, Ginsberg M. Suppression of Integrin Activation: A Novel Function of a Ras/Raf-Initiated MAP Kinase Pathway. Cell 1997, 88: 521-530. PMID: 9038343, DOI: 10.1016/s0092-8674(00)81892-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein KinasesCell SizeCHO CellsCricetinaeCytoplasmDNA, ComplementaryEndoribonucleasesEnzyme ActivationExtracellular Matrix ProteinsFibronectinsFlow CytometryFungal ProteinsGene Expression Regulation, EnzymologicIntegrinsProtein BiosynthesisProtein Serine-Threonine KinasesProtein Structure, TertiaryProto-Oncogene ProteinsProto-Oncogene Proteins c-rafRas ProteinsRecombinant Fusion ProteinsTranscription, GeneticConceptsMAP kinase pathwayKinase pathwayIntegrin activationBeta-subunit cytoplasmic domainH-RasTranscription-independent functionsSubunit cytoplasmic domainERK MAP kinase pathwayIntegrin affinity stateCell adhesion receptorsIntegrin cell adhesion receptorsActivation of integrinsNegative feedback loopSmall GTPCytoplasmic domainEffector kinaseIntegrin phosphorylationRaf-1Novel functionIntegrin functionNegative regulatorAdhesion receptorsProtein synthesisMRNA transcriptionDistinct alpha