2017
Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively Enriched Peripheral Blood Samples
Miller B, Lustberg M, Summers T, Chalmers J. Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively Enriched Peripheral Blood Samples. Methods In Molecular Biology 2017, 1634: 219-234. PMID: 28819855, DOI: 10.1007/978-1-4939-7144-2_19.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorBreast NeoplasmsCarbocyaninesCell CountCell Line, TumorEpithelial Cell Adhesion MoleculeFemaleFluorescent DyesHumansHydrazinesImage Processing, Computer-AssistedIndolesKeratinsLeukocyte Common AntigensLeukocytes, MononuclearLiquid CrystalsNeoplastic Cells, CirculatingOptical ImagingSoftwareSuccinimidesConceptsPeripheral blood samplesBlood samplesPeripheral bloodTumor cellsLiquid biopsyUseful predictive markerSolid tumor malignanciesVariety of disordersUndefined cell typesLow EpCAM expressionSpecific cell surface markersCell surface markersVariety of biomarkersPredictive markerTherapeutic responseSolid tumorsPositive selection technologyDisease statusTumor malignancyFuture validation studiesEpCAM expressionSurface markersMultispectral imaging analysisProtein levelsExact role
2015
Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells
Oliveira-Costa J, de Carvalho A, da Silveira G, Amaya P, Wu Y, Park K, Gigliola M, Lustberg M, Buim M, Ferreira E, Kowalski L, Chalmers J, Soares F, Carraro D, Ribeiro-Silva A. Gene expression patterns through oral squamous cell carcinoma development: PD-L1 expression in primary tumor and circulating tumor cells. Oncotarget 2015, 6: 20902-20920. PMID: 26041877, PMCID: PMC4673238, DOI: 10.18632/oncotarget.3939.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdultAgedAged, 80 and overAutoimmune DiseasesB7-H1 AntigenBiomarkers, TumorCarcinoma, Squamous CellCohort StudiesCytoplasmDNA-Binding ProteinsFemaleGene Expression ProfilingGene Expression Regulation, NeoplasticHomeodomain ProteinsHumansKaplan-Meier EstimateLymphatic MetastasisMaleMiddle AgedMouth NeoplasmsNeoplastic Cells, CirculatingOligonucleotide Array Sequence AnalysisPrognosisProportional Hazards ModelsTissue BanksTreatment OutcomeConceptsOral squamous cell carcinomaPD-L1Tumor sizePerineural invasionPrimary tumorAdvanced oral squamous cell carcinomaTumor cellsSquamous cell carcinoma developmentStrong cytoplasmatic expressionPD-L1 positivityDisease-specific survivalOral squamous cell carcinoma developmentPD-L1 expressionIndependent prognostic factorLymph node metastasisT cell activitySquamous cell carcinomaSub-classify patientsSpecific survivalNode metastasisPD-1Prognostic factorsPoor prognosisAutoimmune diseasesCell carcinoma
2014
A Phase II study of bevacizumab in combination with trastuzumab and docetaxel in HER2 positive metastatic breast cancer
Zhao M, Pan X, Layman R, Lustberg M, Mrozek E, Macrae E, Wesolowski R, Carothers S, Puhalla S, Shapiro C, Ramaswamy B. A Phase II study of bevacizumab in combination with trastuzumab and docetaxel in HER2 positive metastatic breast cancer. Investigational New Drugs 2014, 32: 1285-1294. PMID: 24894652, PMCID: PMC4303337, DOI: 10.1007/s10637-014-0122-5.Peer-Reviewed Original ResearchConceptsProgression-free survivalLeft ventricular ejection fractionClinical benefit rateHER2-positive MBCObjective response rateComplete responsePartial responseBreast cancerStable diseaseFree survivalGrade 3HER2-positive metastatic breast cancerResponse rateAdditional overall survival benefitsMedian progression-free survivalMetastatic breast cancer patientsPositive metastatic breast cancerVascular epithelial growth factorCommon grade 3Cycles of bevacizumabGrade 2 hypertensionMethods Eligible patientsPrior chemotherapy regimensCombination of bevacizumabHand-foot syndromeHeterogeneous atypical cell populations are present in blood of metastatic breast cancer patients
Lustberg M, Balasubramanian P, Miller B, Garcia-Villa A, Deighan C, Wu Y, Carothers S, Berger M, Ramaswamy B, Macrae E, Wesolowski R, Layman R, Mrozek E, Pan X, Summers T, Shapiro C, Chalmers J. Heterogeneous atypical cell populations are present in blood of metastatic breast cancer patients. Breast Cancer Research 2014, 16: r23. PMID: 24602188, PMCID: PMC4053256, DOI: 10.1186/bcr3622.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAntigens, CDAntigens, Differentiation, MyelomonocyticAntigens, NeoplasmBiomarkers, TumorBreast NeoplasmsCell Adhesion MoleculesCell Line, TumorEpithelial Cell Adhesion MoleculeErbB ReceptorsFemaleFlow CytometryHumansImmunohistochemistryKeratin-18Keratin-19Keratin-8Leukocyte Common AntigensMCF-7 CellsMicroscopy, ConfocalMiddle AgedNeoplasm MetastasisNeoplastic Cells, CirculatingPrognosisProspective StudiesVimentinConceptsMetastatic breast cancerBreast cancer patientsBlood samplesCancer patientsBreast cancerMetastatic breast cancer patientsPatient samplesMultiparametric flow cytometry analysisAtypical cell populationNumber of CKPresent prospective trialWorse overall survivalTumor-associated macrophagesCell populationsPan-hematopoietic marker CD45Confocal microscopyEpidermal growth factor receptorEpithelial cell adhesion moleculeNormal control samplesCell surface markersRole of EpCAMFlow cytometry analysisGrowth factor receptorOverall survivalProspective trial
2013
Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology
Wu Y, Deighan C, Miller B, Balasubramanian P, Lustberg M, Zborowski M, Chalmers J. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods 2013, 64: 169-182. PMID: 24056212, PMCID: PMC3874448, DOI: 10.1016/j.ymeth.2013.09.006.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorBreast NeoplasmsCell CountCell Line, TumorFemaleFlow CytometryFluorescent Antibody TechniqueHumansImmunomagnetic SeparationLeukocyte Common AntigensNeoplastic Cells, CirculatingConceptsCancer patientsMetastatic breast cancer patientsBreast cancer patientsPeripheral blood samplesRed blood cell lysisRare cellsNon-hematopoietic cellsBlood cell lysisImmunomagnetic depletionCD45 antibodyBlood samplesLog depletionPatientsImmunofluorescence stainingNucleated cellsWhole bloodBloodCD45RBC lysisCellsCell lysisLysisAntibodies
2012
Multiparameter Analysis, including EMT Markers, on Negatively Enriched Blood Samples from Patients with Squamous Cell Carcinoma of the Head and Neck
Balasubramanian P, Lang J, Jatana K, Miller B, Ozer E, Old M, Schuller D, Agrawal A, Teknos T, Summers T, Lustberg M, Zborowski M, Chalmers J. Multiparameter Analysis, including EMT Markers, on Negatively Enriched Blood Samples from Patients with Squamous Cell Carcinoma of the Head and Neck. PLOS ONE 2012, 7: e42048. PMID: 22844540, PMCID: PMC3406036, DOI: 10.1371/journal.pone.0042048.Peer-Reviewed Original ResearchConceptsEpithelial surface markersN-cadherinEpidermal growth factor receptorGrowth factor receptorSurface markersPositive selection approachEpithelial markersAforementioned proteinsMesenchymal transitionFactor receptorConfocal microscopyCancer metastasisEMT processCancer cellsPhenotypic patternsMesenchymal markersTumor metastasisEnrichment methodologyEMT markersEnrichment technologyCellsEMTExpressionTumor cellsMarkersEmerging Technologies for CTC Detection Based on Depletion of Normal Cells
Lustberg M, Jatana K, Zborowski M, Chalmers J. Emerging Technologies for CTC Detection Based on Depletion of Normal Cells. Recent Results In Cancer Research 2012, 195: 97-110. PMID: 22527498, PMCID: PMC3775349, DOI: 10.1007/978-3-642-28160-0_9.Peer-Reviewed Original Research