2019
RNA–DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast
Hu Y, Bennett HW, Liu N, Moravec M, Williams JF, Azzalin CM, King MC. RNA–DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast. Genetics 2019, 213: 431-447. PMID: 31405990, PMCID: PMC6781888, DOI: 10.1534/genetics.119.302606.Peer-Reviewed Original ResearchConceptsTelomeric repeat-containing RNAHomology-directed repairFission yeastR-loopsLinear chromosomesTelomere maintenanceRecombination-based telomere maintenanceTelomerase-independent telomere maintenanceShelterin component Rap1State of telomeresTelomeric R-loopsRepeat-containing RNARNA-DNA hybridsDeletion of Rap1Telomerase-independent mechanismChromosome endsShelterin componentsGrowth crisisTelomerase functionTelomeric DNARecombination factorsSubset of cancersTelomeresAlternative lengtheningShort telomeres
2018
Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks
Leland BA, Chen AC, Zhao AY, Wharton RC, King MC. Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks. ELife 2018, 7: e33402. PMID: 29697047, PMCID: PMC5945276, DOI: 10.7554/elife.33402.Peer-Reviewed Original ResearchConceptsDNA double-strand breaksDouble-strand breaksLong-range resectionPARPi resistanceBRCA1-deficient cellsHomology-directed repairExonuclease Exo1RecQ helicaseNucleolytic processingMicroscopy assaysREV7Cancer cellsMachineryPreclinical modelsResectionPolymerase inhibitorsPathwayCellsRecQOrthologuesHelicaseExo1AssaysDNA2Breaks
2014
A Role for Nuclear Envelope Bridging Complexes in Homology-Directed Repair
Swartz RK, Rodriguez EC, King MC. A Role for Nuclear Envelope Bridging Complexes in Homology-Directed Repair. Molecular Biology Of The Cell 2014, 25: mbc.e13-10-0569. PMID: 24943839, PMCID: PMC4142617, DOI: 10.1091/mbc.e13-10-0569.Peer-Reviewed Original ResearchConceptsDNA double-strand breaksHomology-directed repairRepair of DSBsNumber of genesDouble-strand breaksSame gene productCytoskeleton (LINC) complexLINC complexGenome instabilityATR kinaseGene conversionKms1DSB resectionGene productsDepolymerization of microtubulesCell cycleCytoplasmic microtubulesG2 phaseDNA damageSad1MicrotubulesComplexesNucleoskeletonMTO1Cytoskeleton