2019
RNA–DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast
Hu Y, Bennett HW, Liu N, Moravec M, Williams JF, Azzalin CM, King MC. RNA–DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast. Genetics 2019, 213: 431-447. PMID: 31405990, PMCID: PMC6781888, DOI: 10.1534/genetics.119.302606.Peer-Reviewed Original ResearchMeSH KeywordsDNA, FungalDNA-Binding ProteinsRecombinational DNA RepairRNA, FungalSchizosaccharomycesSchizosaccharomyces pombe ProteinsShelterin ComplexTelomeraseTelomereTelomere HomeostasisTelomere ShorteningTelomere-Binding ProteinsConceptsTelomeric repeat-containing RNAHomology-directed repairFission yeastR-loopsLinear chromosomesTelomere maintenanceRecombination-based telomere maintenanceTelomerase-independent telomere maintenanceShelterin component Rap1State of telomeresTelomeric R-loopsRepeat-containing RNARNA-DNA hybridsDeletion of Rap1Telomerase-independent mechanismChromosome endsShelterin componentsGrowth crisisTelomerase functionTelomeric DNARecombination factorsSubset of cancersTelomeresAlternative lengtheningShort telomeres
2018
Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks
Leland BA, Chen AC, Zhao AY, Wharton RC, King MC. Rev7 and 53BP1/Crb2 prevent RecQ helicase-dependent hyper-resection of DNA double-strand breaks. ELife 2018, 7: e33402. PMID: 29697047, PMCID: PMC5945276, DOI: 10.7554/elife.33402.Peer-Reviewed Original ResearchMeSH KeywordsCell Cycle ProteinsDNA Breaks, Double-StrandedDNA RepairDNA-Directed DNA PolymeraseMicroscopyNuclear ProteinsRecQ HelicasesSchizosaccharomycesSchizosaccharomyces pombe ProteinsSingle-Cell AnalysisConceptsDNA double-strand breaksDouble-strand breaksLong-range resectionPARPi resistanceBRCA1-deficient cellsHomology-directed repairExonuclease Exo1RecQ helicaseNucleolytic processingMicroscopy assaysREV7Cancer cellsMachineryPreclinical modelsResectionPolymerase inhibitorsPathwayCellsRecQOrthologuesHelicaseExo1AssaysDNA2Breaks
2014
A Role for Nuclear Envelope Bridging Complexes in Homology-Directed Repair
Swartz RK, Rodriguez EC, King MC. A Role for Nuclear Envelope Bridging Complexes in Homology-Directed Repair. Molecular Biology Of The Cell 2014, 25: mbc.e13-10-0569. PMID: 24943839, PMCID: PMC4142617, DOI: 10.1091/mbc.e13-10-0569.Peer-Reviewed Original ResearchMeSH KeywordsCell CycleDNA Breaks, Double-StrandedDNA RepairDNA, FungalDNA, Single-StrandedGenomic InstabilityMicrotubulesNuclear EnvelopeSchizosaccharomycesSchizosaccharomyces pombe ProteinsConceptsDNA double-strand breaksHomology-directed repairRepair of DSBsNumber of genesDouble-strand breaksSame gene productCytoskeleton (LINC) complexLINC complexGenome instabilityATR kinaseGene conversionKms1DSB resectionGene productsDepolymerization of microtubulesCell cycleCytoplasmic microtubulesG2 phaseDNA damageSad1MicrotubulesComplexesNucleoskeletonMTO1CytoskeletonThe KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body
Wälde S, King MC. The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body. Journal Of Cell Science 2014, 127: 3625-3640. PMID: 24963130, PMCID: PMC4132395, DOI: 10.1242/jcs.154997.Peer-Reviewed Original ResearchMeSH KeywordsCell Cycle ProteinsMicrotubule-Associated ProteinsMitosisNuclear ProteinsPhosphoproteinsProtein BindingProtein Serine-Threonine KinasesSchizosaccharomycesSchizosaccharomyces pombe ProteinsSpindle Pole BodiesConceptsSpindle pole bodyMitotic entryPole bodySUN domain protein Sad1Nuclear envelopeMitotic spindle pole bodiesPolo kinase Plo1SUN-KASH complexesYeast centrosome equivalentBipolar spindle formationCentrosome equivalentMitotic remodelingSchizosaccharomyces pombeSpindle formationSpindle polesMonopolar spindlesMitotic catastrophePlo1Cut12PCP1KMS2Sad1PombeBiogenesisRemodeling
2008
A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules
King MC, Drivas TG, Blobel G. A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules. Cell 2008, 134: 427-438. PMID: 18692466, PMCID: PMC2617791, DOI: 10.1016/j.cell.2008.06.022.Peer-Reviewed Original ResearchMeSH KeywordsCell Cycle ProteinsCell NucleusCentromereChromosomal Proteins, Non-HistoneCytoskeletonHeterochromatinMembrane ProteinsMicrotubulesNuclear EnvelopeSchizosaccharomyces pombe ProteinsConceptsSUN-KASH complexesNuclear membrane proteinsMembrane proteinsIntegral inner nuclear membrane proteinsSUN domain protein Sad1Nuclear envelopeSpindle pole body componentInner nuclear membrane proteinFission yeast S. pombeOuter nuclear membrane proteinsCytoplasmic microtubulesYeast S. pombeMicrotubule-dependent forcesNuclear envelope membranesCentromeric DNAS. pombeCentromeric heterochromatinHeterochromatic regionsEnvelope membraneIMA1MicrotubulesSad1HeterochromatinProteinNuclear heterochromatin