2019
Ablation of SUN2-containing LINC complexes drives cardiac hypertrophy without interstitial fibrosis
Stewart RM, Rodriguez EC, King MC. Ablation of SUN2-containing LINC complexes drives cardiac hypertrophy without interstitial fibrosis. Molecular Biology Of The Cell 2019, 30: 1664-1675. PMID: 31091167, PMCID: PMC6727752, DOI: 10.1091/mbc.e18-07-0438.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCardiomegalyCell AdhesionCell Nucleus ShapeDNA-Binding ProteinsFibrosisGene DeletionIntegrinsMAP Kinase Signaling SystemMembrane ProteinsMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesMyocardiumNuclear EnvelopeProto-Oncogene Proteins c-aktSarcomeresTelomere-Binding ProteinsTransforming Growth Factor betaConceptsLINC complexType laminsInner nuclear membrane protein MAN1Nuclear envelopeA-type laminsSarcomeric contractile apparatusNuclear laminaDisease etiologyProfibrotic signalingCytoskeletal linkageAKT/MAPK signalingCardiomyocyte cytoskeletonPlasma membraneMAPK signalingNegative regulatorAntagonistic rolesTGFβ signalingLaminsCellular adhesionSignalingCardiac hypertrophySUN2Human cardiomyopathyHypertrophy markersContractile apparatus
2015
Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions
Stewart RM, Zubek AE, Rosowski KA, Schreiner SM, Horsley V, King MC. Nuclear–cytoskeletal linkages facilitate cross talk between the nucleus and intercellular adhesions. Journal Of Cell Biology 2015, 209: 403-418. PMID: 25963820, PMCID: PMC4427780, DOI: 10.1083/jcb.201502024.Peer-Reviewed Original ResearchConceptsIntercellular adhesionNuclear positionEpidermal tissue integrityLinker of nucleoskeletonCross talkPrimary mouse keratinocytesCytoskeleton (LINC) complexCytoplasmic cytoskeletonAdhesion functionMicrotubule networkDefective adhesionCytoskeletonSUN2Mouse keratinocytesTissue integrityFollicle structureEpidermal keratinocytesAdhesionNucleoskeletonCellsKeratinocytesAdhesion formationNucleusIntegrityComplexes
2008
A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules
King MC, Drivas TG, Blobel G. A Network of Nuclear Envelope Membrane Proteins Linking Centromeres to Microtubules. Cell 2008, 134: 427-438. PMID: 18692466, PMCID: PMC2617791, DOI: 10.1016/j.cell.2008.06.022.Peer-Reviewed Original ResearchConceptsSUN-KASH complexesNuclear membrane proteinsMembrane proteinsIntegral inner nuclear membrane proteinsSUN domain protein Sad1Nuclear envelopeSpindle pole body componentInner nuclear membrane proteinFission yeast S. pombeOuter nuclear membrane proteinsCytoplasmic microtubulesYeast S. pombeMicrotubule-dependent forcesNuclear envelope membranesCentromeric DNAS. pombeCentromeric heterochromatinHeterochromatic regionsEnvelope membraneIMA1MicrotubulesSad1HeterochromatinProteinNuclear heterochromatin
2006
Karyopherin-mediated import of integral inner nuclear membrane proteins
King MC, Lusk C, Blobel G. Karyopherin-mediated import of integral inner nuclear membrane proteins. Nature 2006, 442: 1003-1007. PMID: 16929305, DOI: 10.1038/nature05075.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsInner nuclear membraneINM proteinsMembrane proteinsIntegral inner nuclear membrane proteinsInner nuclear membrane proteinNuclear pore complex proteinsNuclear membrane proteinsRan GTPase cycleBasic sequence motifsNuclear localization signalNuclear pore complexPore complex proteinsAppropriate cellular compartmentDiscrete sequence elementsINM targetingLocalization signalPore complexGTPase cycleNuclear transportSequence motifsCellular compartmentsComplex proteinsSequence elementsKaryopherin β1