2023
Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment
Chiorazzi M, Martinek J, Krasnick B, Zheng Y, Robbins K, Qu R, Kaufmann G, Skidmore Z, Juric M, Henze L, Brösecke F, Adonyi A, Zhao J, Shan L, Sefik E, Mudd J, Bi Y, Goedegebuure S, Griffith M, Griffith O, Oyedeji A, Fertuzinhos S, Garcia-Milian R, Boffa D, Detterbeck F, Dhanasopon A, Blasberg J, Judson B, Gettinger S, Politi K, Kluger Y, Palucka K, Fields R, Flavell R. Autologous humanized PDX modeling for immuno-oncology recapitulates features of the human tumor microenvironment. Journal For ImmunoTherapy Of Cancer 2023, 11: e006921. PMID: 37487666, PMCID: PMC10373695, DOI: 10.1136/jitc-2023-006921.Peer-Reviewed Original ResearchConceptsHuman tumor microenvironmentTumor microenvironmentTumor-immune interactionsSolid tumorsAdaptive immune activationAdaptive immune populationsIndividual tumor microenvironmentsPatient's hematopoietic systemPatient-derived xenograft tissuesVascular endothelial growth factorBone marrow hematopoietic stemBone marrow aspiratePreclinical drug testingEndothelial growth factorHematopoietic systemAutologous tumorPDX modelingPDX miceImmune activationImmune populationsMarrow aspiratesAutologous systemIndividual patientsLittermate controlsPreclinical predictions
2022
Platelet-derived TLT-1 promotes tumor progression by suppressing CD8+ T cells
Tyagi T, Jain K, Yarovinsky TO, Chiorazzi M, Du J, Castro C, Griffin J, Korde A, Martin KA, Takyar SS, Flavell RA, Patel AA, Hwa J. Platelet-derived TLT-1 promotes tumor progression by suppressing CD8+ T cells. Journal Of Experimental Medicine 2022, 220: e20212218. PMID: 36305874, PMCID: PMC9814191, DOI: 10.1084/jem.20212218.Peer-Reviewed Original ResearchConceptsCD8 T cellsT cellsTLT-1Non-small cell lung cancer patientsCell lung cancer patientsTREM-like transcript-1Tumor immunosuppressive mechanismsT cell suppressionLung cancer patientsPatient T cellsNF-κB pathwayPatient-derived tumorsDistinct activation phenotypesNSCLC patientsImmunosuppressive mechanismsSyngeneic tumorsHumanized miceImmunoregulatory rolePrognostic significanceImmunocompetent miceCancer patientsCell suppressionActivation phenotypeReduced tumorTumor growth
2013
Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma
Chiorazzi M, Rui L, Yang Y, Ceribelli M, Tishbi N, Maurer CW, Ranuncolo SM, Zhao H, Xu W, Chan WC, Jaffe ES, Gascoyne RD, Campo E, Rosenwald A, Ott G, Delabie J, Rimsza LM, Shaham S, Staudt LM. Related F-box proteins control cell death in Caenorhabditis elegans and human lymphoma. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 3943-3948. PMID: 23431138, PMCID: PMC3593917, DOI: 10.1073/pnas.1217271110.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApoptosisBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsCaspasesCell Line, TumorEnzyme ActivationF-Box ProteinsHEK293 CellsHumansLymphomaLymphoma, Large B-Cell, DiffuseMolecular Sequence DataMutation, MissenseProto-Oncogene Proteins c-bcl-2Repressor ProteinsRNA, MessengerRNA, NeoplasmSequence Homology, Amino AcidConceptsCED-9Cell deathCaenorhabditis elegansDRE-1Caspase CED-3Control cell deathApoptotic cell deathRegulators of apoptosisEGL-1CED-3Cell fateHuman diffuse large B-cell lymphomaHuman proteinsCaspase activationFBXO10BCL2 proteinElegansProteinHuman malignanciesHuman lymphomasAlternative mechanismApoptosisActivationBH3Regulator
2007
Timing of the onset of a developmental cell death is controlled by transcriptional induction of the C. elegans ced-3 caspase-encoding gene
Maurer CW, Chiorazzi M, Shaham S. Timing of the onset of a developmental cell death is controlled by transcriptional induction of the C. elegans ced-3 caspase-encoding gene. Development 2007, 134: 1357-1368. PMID: 17329362, DOI: 10.1242/dev.02818.Peer-Reviewed Original ResearchConceptsCED-3 caspaseCell deathEGL-1Animal developmentTranscriptional inductionCed-9/BclCED-4/ApafTemporal controlPAL-1Such temporal regulationC. elegans homologCell death onsetDevelopmental expression patternsDevelopmental cell deathCell-specific deathEgl-1 expressionCED-4CED-3CED-9Transcriptional regulationSomatic cellsTemporal regulationPromoter sitesExpression patternsTranscription