2000
Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides
DiGiovanna M, Roussel R, Stern D. Production of Antibodies That Recognize Specific Tyrosine‐Phosphorylated Peptides. Current Protocols In Molecular Biology 2000, 50: 18.6.1-18.6.19. PMID: 18265171, DOI: 10.1002/0471142727.mb1806s50.BooksConceptsAnti-phosphopeptide antibodiesIndividual phosphorylation sitesAnalysis of phosphoproteinsAnti-phosphoamino acid antibodiesPhosphorylation sitesUnphosphorylated proteinTyrosine phosphorylated peptidesCognate proteinPhosphorylated stateParticular proteinAffinity matrixProteinUnique specificityPhosphoproteinFunctional stateDifferential isolationSuch reagentsPhosphotyrosinePeptidesSupport protocolSpeciesAbundanceProduction of antibodiesConventional antibodies
1992
Differentiation stage specificity of tyrosine phosphorylation in response to granulocyte macrophage colony stimulating factor (GM-CSF)
DiGiovanna M, Sartorelli A. Differentiation stage specificity of tyrosine phosphorylation in response to granulocyte macrophage colony stimulating factor (GM-CSF). Cancer Letters 1992, 63: 15-22. PMID: 1555204, DOI: 10.1016/0304-3835(92)90084-9.Peer-Reviewed Original ResearchConceptsTyrosine protein kinase activityProtein kinase activityTyrosine phosphorylationKinase activityWEHI-3B cellsMyeloid differentiationUndifferentiated cellsFunctional roleTransduction of signalsDifferentiation stage specificityEndogenous cellular proteinsTyrosine protein kinaseGM-CSF receptorGranulocyte-macrophage colonyMacrophage colonyCellular proteinsMyeloid cellsProtein kinaseMature myeloid cellsStage specificityGM-CSFDifferentiation processWEHI-3BMature cellsPhosphorylation