2020
Lymphotoxin targeted to salivary and lacrimal glands induces tertiary lymphoid organs and cervical lymphadenopathy and reduces tear production
Truman LA, Bentley KL, Ruddle NH. Lymphotoxin targeted to salivary and lacrimal glands induces tertiary lymphoid organs and cervical lymphadenopathy and reduces tear production. European Journal Of Immunology 2020, 50: 418-425. PMID: 32012252, DOI: 10.1002/eji.201948300.Peer-Reviewed Original ResearchConceptsTertiary lymphoid organsLacrimal glandCervical lymphadenopathySjögren's syndromeLymphoid organsTear productionRole of lymphotoxinTLO formationAutoantibody titresMALT lymphomaLymphoid tissueTransgenic miceLymphotoxinMiceLymphadenopathyGlandSyndromeOrgansAutoimmunityMucosalLymphomaLTαTitresSalivaryLTβ
2014
Pillars article: Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin. Science. 1994. 264: 703-707.
De Togni P, Goellner J, Ruddle NH, Streeter PR, Andrea F, Mariathasan S, Smith SC, Carlson R, Shornick LP, Strauss-Schoenberger J, Russell JH, Karr R, Chaplin DD. Pillars article: Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin. Science. 1994. 264: 703-707. The Journal Of Immunology 2014, 192: 2010-4. PMID: 24563504.Peer-Reviewed Original Research
2013
Lymphatic Vessel Function in Head and Neck Inflammation
Truman LA, A-Gonzalez N, Bentley KL, Ruddle NH. Lymphatic Vessel Function in Head and Neck Inflammation. Lymphatic Research And Biology 2013, 11: 187-192. PMID: 24044758, PMCID: PMC3780307, DOI: 10.1089/lrb.2013.0013.Peer-Reviewed Original ResearchConceptsIndividual lymphatic endothelial cellsLymphatic endothelial cellsRed fluorescent reporterEndothelial cellsLymphatic vesselsTranscription factorsRegulatory elementsFaithful expressionProx1 expressionLymphatic vessel functionSingle cellsReporter miceLymphangiogenesisTd-TomatoJackson LaboratoryCellsVivoTdTomatoExpressionProx1TransgeneReporterImmune responseVessel functionMice
2012
ProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets
Truman LA, Bentley KL, Smith EC, Massaro SA, Gonzalez DG, Haberman AM, Hill M, Jones D, Min W, Krause DS, Ruddle NH. ProxTom Lymphatic Vessel Reporter Mice Reveal Prox1 Expression in the Adrenal Medulla, Megakaryocytes, and Platelets. American Journal Of Pathology 2012, 180: 1715-1725. PMID: 22310467, PMCID: PMC3349900, DOI: 10.1016/j.ajpath.2011.12.026.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal MedullaAnimalsBlood PlateletsCells, CulturedCytoplasmEndothelial CellsGene Expression RegulationGenotypeGlycoproteinsHomeodomain ProteinsLuminescent ProteinsLymph NodesLymphatic VesselsMegakaryocytesMembrane Transport ProteinsMiceMice, Inbred C57BLMice, TransgenicMicroscopy, FluorescenceTumor Cells, CulturedTumor Suppressor ProteinsConceptsLymph nodesLymphatic vesselsAdrenal medullaExpression of Prox1Tumor metastasisHigh endothelial venulesProx1 expressionTwo-photon laser scanning microscopyTransplant rejectionDentate gyrusEndothelial venulesAntigen presentationC57BL/6 backgroundTransgenic miceLipid metabolismMiceNeuroendocrine cellsAdult liverNovel siteMetastasisMedullaStudy of diseasesLiving mouseUnknown rolePotential utility
2011
Resident B cells regulate thymic expression of myelin oligodendrocyte glycoprotein
Akirav EM, Xu Y, Ruddle NH. Resident B cells regulate thymic expression of myelin oligodendrocyte glycoprotein. Journal Of Neuroimmunology 2011, 235: 33-39. PMID: 21550671, PMCID: PMC3157307, DOI: 10.1016/j.jneuroim.2011.03.013.Peer-Reviewed Original ResearchConceptsB cellsB cell-deficient μMT miceMyelin oligodendrocyte glycoproteinResident B cellsThymic B cellsCortico-medullary junctionMinor cell populationProduction of LTInsulin mRNA expressionΜMT miceEpithelial cell numberOligodendrocyte glycoproteinThymic expressionAntigen expressionMRNA expressionNormal tissuesCell populationsCell numberCellsExpressionLtUnexpected roleBiological roleLymphotoxinMice
2009
Depletion of CD4+CD25+ T cells exacerbates experimental autoimmune encephalomyelitis induced by mouse, but not rat, antigens
Akirav EM, Bergman CM, Hill M, Ruddle NH. Depletion of CD4+CD25+ T cells exacerbates experimental autoimmune encephalomyelitis induced by mouse, but not rat, antigens. Journal Of Neuroscience Research 2009, 87: 3511-3519. PMID: 19125411, PMCID: PMC4429897, DOI: 10.1002/jnr.21981.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigensAutoantigensAutoimmunityBiomarkersCD4 AntigensCD4-Positive T-LymphocytesCells, CulturedCentral Nervous SystemChemotaxis, LeukocyteDisease Models, AnimalEncephalomyelitis, Autoimmune, ExperimentalFemaleInterferon-gammaInterleukin-10Interleukin-17Interleukin-2 Receptor alpha SubunitLymphocyte ActivationMiceMice, Inbred C57BLMultiple SclerosisMyelin ProteinsMyelin-Associated GlycoproteinMyelin-Oligodendrocyte GlycoproteinRatsT-Lymphocytes, RegulatoryConceptsExperimental autoimmune encephalomyelitisMyelin oligodendrocyte glycoproteinAutoimmune encephalomyelitisT cellsIL-10-producing cellsRegulatory T cellsTissue-restricted antigensCentral nervous systemField of autoimmunityT cell activationTreg depletionEAE severitySelf antigensOligodendrocyte glycoproteinForeign antigensExperimental diseaseNervous systemRelated antigensMiceSelf-antigen specificityAntigenTregsEncephalomyelitisAutoimmunityRats
2007
Regulatory T cells selectively protect against experimental autoimmune encephalomyelitis induced with self but not foreign antigens (B79)
Akirav E, Xu Y, Bergman C, Hill M, Ruddle N. Regulatory T cells selectively protect against experimental autoimmune encephalomyelitis induced with self but not foreign antigens (B79). The Journal Of Immunology 2007, 178: lb16-lb17. DOI: 10.4049/jimmunol.178.supp.b79.Peer-Reviewed Original ResearchMouse myelin oligodendrocyte glycoproteinExperimental autoimmune encephalomyelitisMyelin oligodendrocyte glycoproteinRegulatory T cellsTreg depletionT cellsAutoimmune encephalomyelitisMultiple sclerosisHuman myelin oligodendrocyte glycoproteinRatio of IFNγT cell responsesT cell proliferationCentral nervous systemEAE severityMOG35-55IL17 productionAutoimmune diseasesOligodendrocyte glycoproteinForeign antigensMurine modelNervous systemRelated antigensDisease severityCell responsesMice
2005
Chronic Lymphocytic Inflammation Specifies the Organ Tropism of Prions
Heikenwalder M, Zeller N, Seeger H, Prinz M, Klöhn P, Schwarz P, Ruddle NH, Weissmann C, Aguzzi A. Chronic Lymphocytic Inflammation Specifies the Organ Tropism of Prions. Science 2005, 307: 1107-1110. PMID: 15661974, DOI: 10.1126/science.1106460.Peer-Reviewed Original ResearchConceptsInflammatory conditionsNormal cellular prion protein PrPCCellular prion protein PrPCChronic lymphocytic inflammationIatrogenic prion transmissionChronic inflammatory conditionsPrion protein PrPCFDC-M1Lymphocytic inflammationEctopic inductionProinflammatory cytokinesInflamed organsImmune cellsInflammatory diseasesInflammatory fociLymphoid tissuePrion accumulationPrion inoculationOrgan tropismPrion pathogenesisPrion replicationTissue distributionPrion transmissionPrionsMice
2004
Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates Relationship to the MECA-79 Epitope
Bistrup A, Tsay D, Shenoy P, Singer MS, Bangia N, Luther SA, Cyster JG, Ruddle NH, Rosen SD. Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates Relationship to the MECA-79 Epitope. American Journal Of Pathology 2004, 164: 1635-1644. PMID: 15111310, PMCID: PMC1615668, DOI: 10.1016/s0002-9440(10)63722-4.Peer-Reviewed Original ResearchConceptsHigh endothelial venulesMECA-79 epitopeLymph nodesMECA-79Endothelial venulesHEC-GlcNAc6STHigh Endothelial Venule-Like VesselsLuminal stainingL-selectinMECA-79 monoclonal antibodyNull miceNonobese diabetic (NOD) miceHEV-like vesselsLymph nodes resultsHigh endothelial cellsLymphoid neogenesisDiabetic miceLymphocyte rollingLymphocyte homingMonoclonal antibodiesEndothelial cellsMiceConcomitant expressionEpitopesVenules
2003
Rat and Human Myelin Oligodendrocyte Glycoproteins Induce Experimental Autoimmune Encephalomyelitis by Different Mechanisms in C57BL/6 Mice
Oliver A, Lyon G, Ruddle N. Rat and Human Myelin Oligodendrocyte Glycoproteins Induce Experimental Autoimmune Encephalomyelitis by Different Mechanisms in C57BL/6 Mice. The Journal Of Immunology 2003, 171: 4934-4934. DOI: 10.4049/jimmunol.171.9.4934.Peer-Reviewed Original Research
2001
Lymphotoxin-alpha deficiency completely protects C57BL/6 mice from developing clinical experimental autoimmune myasthenia gravis
Goluszko E, Hjelmström P, Deng C, Poussin M, Ruddle N, Christadoss P. Lymphotoxin-alpha deficiency completely protects C57BL/6 mice from developing clinical experimental autoimmune myasthenia gravis. Journal Of Neuroimmunology 2001, 113: 109-118. PMID: 11137582, DOI: 10.1016/s0165-5728(00)00420-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDAutoantibodiesB7-2 AntigenGene ExpressionImmunodominant EpitopesImmunoglobulin GImmunoglobulin MLymphotoxin-alphaMembrane GlycoproteinsMiceMice, Inbred C57BLMice, KnockoutMyasthenia Gravis, Autoimmune, ExperimentalReceptors, CholinergicReceptors, Tumor Necrosis FactorSpleenConceptsExperimental autoimmune myasthenia gravisClinical experimental autoimmune myasthenia gravisAutoimmune myasthenia gravisMyasthenia gravisMean titersPrimary humoral immune responseAlpha-deficient miceAnti-AChR antibodiesHumoral immune responseLower mean titersC57BL/6 miceImmunized miceTotal IgGDeficient miceIgG isotypeImmune responseAcetylcholine receptorsPartial preventionGravisMiceComplete preventionTitersLtPreventionPathogenesis
1999
Lymphoid neoorganogenesis
Ruddle N. Lymphoid neoorganogenesis. Immunologic Research 1999, 19: 119-125. PMID: 10493167, DOI: 10.1007/bf02786481.Peer-Reviewed Original ResearchConceptsVascular cell adhesion moleculeE-selectin adhesion moleculesIntercellular adhesion moleculePeripheral node addressinAdhesion moleculesKnockout miceTertiary lymphoid organsCellular adhesion moleculesNecrosis factor familyLymphoid organ developmentEndothelial cell lineAutoimmune diseasesChronic inflammationLymphoid organsLymphoid tissueCell adhesion moleculeLymphotoxinInflammationMultiple receptorsMicrobial infectionsCell linesMiceOrgan developmentFactor familyAddressin
1997
Distinct Roles in Lymphoid Organogenesis for Lymphotoxins α and β Revealed in Lymphotoxin β–Deficient Mice
Koni P, Sacca R, Lawton P, Browning J, Ruddle N, Flavell R. Distinct Roles in Lymphoid Organogenesis for Lymphotoxins α and β Revealed in Lymphotoxin β–Deficient Mice. Immunity 1997, 6: 491-500. PMID: 9133428, DOI: 10.1016/s1074-7613(00)80292-7.Peer-Reviewed Original ResearchConceptsMesenteric lymph nodesLT alpha-deficient miceAlpha-deficient miceFollicular dendritic cellsBeta-deficient miceLymph nodesDendritic cellsDeficient miceLymphoid organogenesisTumor necrosis factor receptor type ILTbeta-deficient micePeripheral lymph nodesReceptor type ISplenic germinal centersLymphotoxin βPeyer's patchesGerminal centersLymphotoxin alphaLT-alphaLT alpha3Lymphotoxin αMiceUnidentified receptorType IAlpha
1996
The Contribution of Insulitis to Diabetes Development in Tumor Necrosis Factor Transgenic Mice
Flavell RA, Kratz A, Ruddle NH. The Contribution of Insulitis to Diabetes Development in Tumor Necrosis Factor Transgenic Mice. Current Topics In Microbiology And Immunology 1996, 206: 33-50. PMID: 8608724, DOI: 10.1007/978-3-642-85208-4_3.Peer-Reviewed Original ResearchConceptsInsulin-dependent diabetes mellitusTumor necrosis factor-transgenic (TNF-Tg) miceDevelopment of diabetesHLA susceptibility allelesIslets of LangerhansHLA-DQβDiabetes mellitusFrank diabetesTransgenic miceStrong associationSusceptibility allelesDiabetesIdentical twinsDiseaseInsulinIsletsInsulitisSite of synthesisMellitusNumber of yearsPatientsMiceEnvironmental factors
1994
10 Studies of Tolerance, Inflammation, and Autoimmunity in Transgenic Mice
Antonia S, Elliott E, Guerder S, Picarella D, Ruddle N, Flavell R. 10 Studies of Tolerance, Inflammation, and Autoimmunity in Transgenic Mice. 1994, 155-174. DOI: 10.1016/b978-0-12-105760-2.50014-5.Peer-Reviewed Original ResearchAutoreactive T cellsT cellsTransgenic miceT antigen transgenic miceLocal antigen presentationSpecific T cellsInfluence of cytokinesAutoreactive cellsAntigen presentationTissue destructionEffective antigenAutoimmunityClonal expansionMiceTissue remodelingStudy of toleranceAntigenIsletsCellsTissueActivationInflammationHyperplasiaCytokinesExpression
1992
Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas.
Picarella DE, Kratz A, Li CB, Ruddle NH, Flavell RA. Insulitis in transgenic mice expressing tumor necrosis factor beta (lymphotoxin) in the pancreas. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 10036-10040. PMID: 1279667, PMCID: PMC50272, DOI: 10.1073/pnas.89.21.10036.Peer-Reviewed Original ResearchConceptsNecrosis factor betaTransgenic miceFactor betaInsulin-dependent diabetes mellitusRat insulin II promoterTumor necrosis factor betaType 1 diabetesRegulation of inflammationTNF-beta geneDiabetes mellitusInflammatory infiltrateInflammatory diseasesT cellsImmune responseB cellsInsulitisDiabetesMicePancreasImportant early stepBetaEarly stagesCD8InfiltratesMellitus
1977
Role of Endogenous Murine Leukemia Virus in Immunologically Triggered Lymphoreticular Tumors. I. Development and Use of Oncogenic Cellfree Preparations Serially Passaged In Vivo2
Armstrong M, Ruddle N, Lipman M, Pierce S, Richards F. Role of Endogenous Murine Leukemia Virus in Immunologically Triggered Lymphoreticular Tumors. I. Development and Use of Oncogenic Cellfree Preparations Serially Passaged In Vivo2. Journal Of The National Cancer Institute 1977, 58: 67-72. PMID: 13227, DOI: 10.1093/jnci/58.1.67.Peer-Reviewed Original ResearchConceptsLymphoreticular tumorsB-tropic MuLVSyngeneic miceBALB/Reticular tissueMurine leukemia virusYoung adult recipientsLatent periodNormal control miceLeukemia virusEndogenous murine leukemia virusMean latent periodImmunologic disturbancesAdult recipientsControl miceMuLV replicationHost reactionGVHRTumorsMiceB-tropic murine leukemia virusSerial passageCellfree extractsMuLVVirus