Methylation-induced silencing of ASC/TMS1, a pro-apoptotic gene, is a late-stage event in colorectal cancer
Riojas MA, Guo M, Glöckner SC, Machida EO, Baylin SB, Ahuja N. Methylation-induced silencing of ASC/TMS1, a pro-apoptotic gene, is a late-stage event in colorectal cancer. Cancer Biology & Therapy 2007, 6: 1710-1716. PMID: 17986858, DOI: 10.4161/cbt.6.11.4829.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedCARD Signaling Adaptor ProteinsCell Line, TumorColorectal NeoplasmsCytoskeletal ProteinsDNA MethylationFemaleGene SilencingHumansMaleMiddle AgedNeoplasm StagingReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTumor Necrosis Factor-alphaConceptsASC/TMS1Normal colorectal tissue samplesCRC cell linesColorectal tissue samplesColorectal cancerColorectal adenomasMethylation-specific PCRCell linesMRNA expressionPro-apoptotic genesPrimary colorectal cancer specimensStage 1 cancerStage 2 cancerStage 3 cancerRight-sided tumorsPrimary colorectal cancerStage 4 cancerColorectal cancer specimensTissue samplesColorectal cancer cell linesNormal colorectal tissuesUnmethylated cell linesDifferent human neoplasmsCancer cell linesLate-stage eventComparing the DNA Hypermethylome with Gene Mutations in Human Colorectal Cancer
Schuebel KE, Chen W, Cope L, Glöckner SC, Suzuki H, Yi JM, Chan TA, Van Neste L, Van Criekinge W, van den Bosch S, van Engeland M, Ting AH, Jair K, Yu W, Toyota M, Imai K, Ahuja N, Herman JG, Baylin SB. Comparing the DNA Hypermethylome with Gene Mutations in Human Colorectal Cancer. PLOS Genetics 2007, 3: e157. PMID: 17892325, PMCID: PMC1988850, DOI: 10.1371/journal.pgen.0030157.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceColorectal NeoplasmsCpG IslandsDNA MethylationDNA PrimersHumansMutationPromoter Regions, GeneticReverse Transcriptase Polymerase Chain ReactionConceptsTranscriptome-wide approachCpG island DNA hypermethylationHuman colorectal cancer samplesHuman cancer genomesTumor-specific hypermethylationEpigenetic screensTranscriptional silencingIndividual genesCancer genomesEpigenetic changesDNA hypermethylationGene mutationsGenesHypermethylationCell linesIndividual tumorsHuman colorectal cancerColorectal cancer samplesCancer samplesMutationsColorectal cancerCancer biomarkersGenomeSilencingPromoter