Featured Publications
Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients
Qu R, Gupta K, Dong D, Jiang Y, Landa B, Saez C, Strickland G, Levinsohn J, Weng PL, Taketo MM, Kluger Y, Myung P. Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients. Developmental Cell 2022, 57: 1053-1067.e5. PMID: 35421372, PMCID: PMC9050909, DOI: 10.1016/j.devcel.2022.03.011.Peer-Reviewed Original ResearchConceptsMorphogen gradientsCell fate specificationWnt/β-cateninFate specificationShape tissuesOrgan formationCell fateDermal condensatesGenetic perturbationsNiche formationDifferentiation processSpatiotemporal patterningCell behaviorΒ-cateninMore intermediatesComputational approachProliferationMorphogenesisScRNAOrganogenesisShhKey componentProgenitorsCritical transitionDKK1
2021
Inference and analysis of cell-cell communication using CellChat
Jin S, Guerrero-Juarez CF, Zhang L, Chang I, Ramos R, Kuan CH, Myung P, Plikus MV, Nie Q. Inference and analysis of cell-cell communication using CellChat. Nature Communications 2021, 12: 1088. PMID: 33597522, PMCID: PMC7889871, DOI: 10.1038/s41467-021-21246-9.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencing dataCell-cell communicationEffective systems-level analysesDatabase of interactionsRNA sequencing dataIntercellular communication networksContext-specific pathwaysCellChatDiverse tissuesIntercellular communicationSystem-level analysisSignaling linkNetwork analysisPathwayCellsMolecular complexesCofactorComplexesReceptors
2015
Niche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool
Mesa KR, Rompolas P, Zito G, Myung P, Sun TY, Brown S, Gonzalez DG, Blagoev KB, Haberman AM, Greco V. Niche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature 2015, 522: 94-97. PMID: 25849774, PMCID: PMC4457634, DOI: 10.1038/nature14306.Peer-Reviewed Original Research
2011
Coordinated Activation of Wnt in Epithelial and Melanocyte Stem Cells Initiates Pigmented Hair Regeneration
Rabbani P, Takeo M, Chou W, Myung P, Bosenberg M, Chin L, Taketo MM, Ito M. Coordinated Activation of Wnt in Epithelial and Melanocyte Stem Cells Initiates Pigmented Hair Regeneration. Cell 2011, 145: 941-955. PMID: 21663796, PMCID: PMC3962257, DOI: 10.1016/j.cell.2011.05.004.Peer-Reviewed Original ResearchConceptsSecondary hair germMelanocyte stem cellsStem cellsStem cell behaviorStem cell populationHair regenerationHair follicle formationPigment-producing melanocytesHair follicle regenerationHair follicle bulgeEpithelial stem cellsGenetic mouse modelsCoordinated activationWntKey pathwaysCell behaviorWnt activationFollicle bulgeFollicle regenerationComplex organHair germFollicle formationCell populationsMcSCsCells
2004
Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function* [boxs]
Singer AL, Bunnell SC, Obstfeld AE, Jordan MS, Wu JN, Myung PS, Samelson LE, Koretzky GA. Roles of the Proline-rich Domain in SLP-76 Subcellular Localization and T Cell Function* [boxs]. Journal Of Biological Chemistry 2004, 279: 15481-15490. PMID: 14722089, DOI: 10.1074/jbc.m313339200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAlanineAntigens, CDAntigens, Differentiation, T-LymphocyteArginineBlotting, WesternCalciumCell LineCell LineageFlow CytometryGene DeletionGenes, DominantHematopoietic Stem CellsHumansJurkat CellsLectins, C-TypeLuciferasesLymphocyte ActivationLysineMembrane MicrodomainsModels, BiologicalMutationPhosphoproteinsPlasmidsPrecipitin TestsProlineProtein Structure, TertiarySignal TransductionSrc Homology DomainsSubcellular FractionsTime FactorsT-LymphocytesTransfectionConceptsSLP-76P1 domainSLP-76 functionProline-rich domainProline-rich regionDomain deletion mutantMultiple hematopoietic lineagesLeukocyte-specific phosphoproteinSrc homologyDeletion mutantsSignal transductionSubcellular localizationHematopoietic lineagesFunctional roleProtein fragmentsT cell receptorMolecular scaffoldsCell functionCell receptorLocalizationComplementary approachesDomainT cell functionMolecular associationDirect evidence
2003
Macrophage activation and Fcγ receptor-mediated signaling do not require expression of the SLP-76 and SLP-65 adaptors
Nichols KE, Haines K, Myung PS, Newbrough S, Myers E, Jumaa H, Shedlock DJ, Shen H, Koretzky GA. Macrophage activation and Fcγ receptor-mediated signaling do not require expression of the SLP-76 and SLP-65 adaptors. Journal Of Leukocyte Biology 2003, 75: 541-552. PMID: 14694181, DOI: 10.1189/jlb.0703312.Peer-Reviewed Original ResearchConceptsBone marrow-derived macrophagesSLP-76SLP-65Extracellular signaling-regulated kinase 1Sheep red blood cellsInitial host defenseLow-dose infectionNumber of liverWild-type miceSrc homology 2Functional eventsMarrow-derived macrophagesTotal body burdenReceptor-mediated signalingReceptor-induced activationRed blood cellsLeukocyte-specific phosphoproteinReactive oxygen intermediatesT lymphocytesMast cellsFc receptorsMacrophage activationListeria monocytogenesHost defenseColony-forming units
2002
Differential Requirement for LAT and SLP-76 in GPVI versus T Cell Receptor Signaling
Judd BA, Myung PS, Obergfell A, Myers EE, Cheng AM, Watson SP, Pear WS, Allman D, Shattil SJ, Koretzky GA. Differential Requirement for LAT and SLP-76 in GPVI versus T Cell Receptor Signaling. Journal Of Experimental Medicine 2002, 195: 705-717. PMID: 11901197, PMCID: PMC2193740, DOI: 10.1084/jem.20011583.Peer-Reviewed Original Research
2001
Differential Requirement for SLP-76 Domains in T Cell Development and Function
Myung P, Derimanov G, Jordan M, Punt J, Liu Q, Judd B, Meyers E, Sigmund C, Freedman B, Koretzky G. Differential Requirement for SLP-76 Domains in T Cell Development and Function. Immunity 2001, 15: 1011-1026. PMID: 11754821, DOI: 10.1016/s1074-7613(01)00253-9.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAmino Acid MotifsAmino Acid SubstitutionAnimalsBinding SitesCalcium SignalingCarrier ProteinsCD3 ComplexCell DifferentiationClonal DeletionImmunophenotypingMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicMutation, MissensePhosphoproteinsProtein Structure, TertiaryReceptors, Antigen, T-CellRecombinant Fusion ProteinsSequence DeletionSignal TransductionSpleenSrc Homology DomainsStructure-Activity RelationshipThymus GlandT-LymphocytesConceptsSLP-76T cell developmentCell developmentDifferential requirementSLP-76 functionT cell receptor signalingCell receptor signalingAdaptor proteinMolecular mechanismsNull backgroundExamination of miceReceptor signalingCell functionPeripheral T cellsNew insightsTransgenic miceStructural requirementsSpecific domainsT cell functionT cellsCellsDomainMutantsThymocytesSignalingPositive and negative regulation of t-cell activation by adaptor proteins
Koretzky G, Myung P. Positive and negative regulation of t-cell activation by adaptor proteins. Nature Reviews Immunology 2001, 1: 95-107. PMID: 11905825, DOI: 10.1038/35100523.Peer-Reviewed Original ResearchConceptsEffector protein activityPost-translational modificationsSrc family kinasesAdaptor domainsAdaptor proteinSH3 domainFamily kinasesSubcellular localizationGenetic manipulationNegative regulationProtein activityNegative regulatorAdaptorEffector moleculesT cell activationCell linesSpatial organizationRegulatorProteinIntramolecular interactionsLymphocyte activationSH2ActivationKinaseComplexesCoupling of the TCR to Integrin Activation by SLAP-130/Fyb
Peterson E, Woods M, Dmowski S, Derimanov G, Jordan M, Wu J, Myung P, Liu Q, Pribila J, Freedman B, Shimizu Y, Koretzky G. Coupling of the TCR to Integrin Activation by SLAP-130/Fyb. Science 2001, 293: 2263-2265. PMID: 11567141, DOI: 10.1126/science.1063486.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAnimalsAntigens, CDAntigens, Differentiation, T-LymphocyteCarrier ProteinsCD3 ComplexCell AdhesionCell MembraneImmunologic CappingIntercellular Adhesion Molecule-1Interleukin-2Lectins, C-TypeLymphocyte ActivationLymphocyte Function-Associated Antigen-1MicePhosphatidylinositol 3-KinasesPhosphoproteinsProtein-Tyrosine KinasesReceptors, Antigen, T-CellReceptors, Interleukin-2Signal TransductionT-LymphocytesSeparation of Notch1 Promoted Lineage Commitment and Expansion/Transformation in Developing T Cells
Allman D, Karnell F, Punt J, Bakkour S, Xu L, Myung P, Koretzky G, Pui J, Aster J, Pear W. Separation of Notch1 Promoted Lineage Commitment and Expansion/Transformation in Developing T Cells. Journal Of Experimental Medicine 2001, 194: 99-106. PMID: 11435476, PMCID: PMC2193437, DOI: 10.1084/jem.194.1.99.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowCell LineageDNA-Binding ProteinsHematopoietic Stem CellsHyaluronan ReceptorsLeukemia, T-CellMembrane ProteinsMiceMice, TransgenicReceptor, Notch1Receptors, Antigen, T-Cell, alpha-betaReceptors, Cell SurfaceReceptors, Interleukin-2Signal TransductionThymus GlandT-LymphocytesTranscription FactorsConceptsT lineage commitmentT cell developmentHematopoietic stem cellsLineage commitmentCell developmentSrc homology 2 domainPre-T cell receptorBone marrowT cell-specific signalsBM transferT cellsCell-specific signalsMultipotent progenitor cellsDouble-positive T cellsTCR-beta transgeneLeukocyte proteinBM cell populationsFunction of Notch1T-cell leukemiaLater time pointsCD3 epsilonActive Notch1Beta transgeneBM cellsCell leukemiaNotch1 Regulates Maturation of CD4+ and CD8+ Thymocytes by Modulating TCR Signal Strength
Izon D, Punt J, Xu L, Karnell F, Allman D, Myung P, Boerth N, Pui J, Koretzky G, Pear W. Notch1 Regulates Maturation of CD4+ and CD8+ Thymocytes by Modulating TCR Signal Strength. Immunity 2001, 14: 253-264. PMID: 11290335, DOI: 10.1016/s1074-7613(01)00107-8.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDAntigens, Differentiation, T-LymphocyteCD4-Positive T-LymphocytesCD5 AntigensCD8-Positive T-LymphocytesCell DifferentiationDNA-Binding ProteinsFlow CytometryGene Expression RegulationHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansJurkat CellsLectins, C-TypeLiverMembrane ProteinsMiceMice, TransgenicNFATC Transcription FactorsNuclear ProteinsPromoter Regions, GeneticReceptor, Notch1Receptors, Antigen, T-CellReceptors, Cell SurfaceResponse ElementsSignal TransductionThymus GlandTranscription Factor AP-1Transcription FactorsConceptsTCR signal strengthCell fate decisionsJurkat T cellsTCR-mediated signalingT cell developmentFate decisionsMultiple lineagesNotch signalingDevelopmental arrestCell developmentNotch expressionThymocyte developmentTCR stimulationRetroviral expressionT cellsPhysiological regulationSingle-positive T cellsTCR transgenic thymocytesDifferentiation of immatureTransgenic thymocytesNotch1Maturation of CD4SignalingMouse thymocytesThymocytes
2000
Hematopoietic reconstitution of SLP-76 corrects hemostasis and platelet signaling through αIIbβ3 and collagen receptors
Judd B, Myung P, Leng L, Obergfell A, Pear W, Shattil S, Koretzky G. Hematopoietic reconstitution of SLP-76 corrects hemostasis and platelet signaling through αIIbβ3 and collagen receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 12056-12061. PMID: 11050236, PMCID: PMC17293, DOI: 10.1073/pnas.97.22.12056.Peer-Reviewed Original ResearchConceptsSLP-76Alpha IIbAdapter protein SLP-76SLP-76 functionSLP-76-deficient miceRapid tyrosine phosphorylationSLP-76-deficient plateletsTyrosine phosphorylated proteinsCollagen receptorCollagen receptor signalingT cell developmentPlatelet fibrinogen receptorPhosphorylated proteinsActin rearrangementCell peripheryLamellipodial extensionTyrosine phosphorylationCell developmentMurine plateletsRetroviral transductionReceptor signalingBind fibrinogenHematopoietic cellsFibrinogen receptorPlatelet-bound fibrinogenAdapter proteins in lymphocyte antigen-receptor signaling
Myung P, Boerthe N, Koretzky G. Adapter proteins in lymphocyte antigen-receptor signaling. Current Opinion In Immunology 2000, 12: 256-266. PMID: 10781404, DOI: 10.1016/s0952-7915(00)00085-6.Peer-Reviewed Original Research