2022
Significance of KRAS mutation testing in biliary brushing cytology specimens: A 10‐year retrospective review
Sun T, Zuo T, Hui P, Cai G. Significance of KRAS mutation testing in biliary brushing cytology specimens: A 10‐year retrospective review. Cancer Cytopathology 2022, 130: 558-565. PMID: 35417072, DOI: 10.1002/cncy.22579.Peer-Reviewed Original ResearchConceptsExtrahepatic biliary stenosisKRAS mutation testingKRAS mutational analysisKRAS mutationsBiliary stenosisBiliary ductsInstitutional review board approvalResults of cytologyPancreatic duct adenocarcinomaBiliary brushing cytologyReview board approvalCytological diagnostic categoriesRetrospective reviewBiliary stricturesDuct adenocarcinomaAbsolute riskCytology examinationDuct brushingsBiliary brushingsBrushing cytologyMalignant conditionsCytological diagnosisBoard approvalClinical practiceCytology specimensSaliVISION: a rapid saliva-based COVID-19 screening and diagnostic test with high sensitivity and specificity
DeFina SM, Wang J, Yang L, Zhou H, Adams J, Cushing W, Tuohy B, Hui P, Liu C, Pham K. SaliVISION: a rapid saliva-based COVID-19 screening and diagnostic test with high sensitivity and specificity. Scientific Reports 2022, 12: 5729. PMID: 35388102, PMCID: PMC8986854, DOI: 10.1038/s41598-022-09718-4.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Severe acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Diagnostic testingRespiratory syndrome coronavirus 2Reverse transcription loop-mediated isothermal amplificationTranscription loop-mediated isothermal amplificationTranscription-quantitative polymerase chain reactionSyndrome coronavirus 2COVID-19 diagnostic testingCoronavirus disease 2019Public health challengeRapid diagnostic testingCOVID-19 screeningQuantitative polymerase chain reactionLoop-mediated isothermal amplificationCoronavirus 2Polymerase chain reactionLaboratory testingRapid diagnostic assaysDisease 2019RT-LAMPInvasive sample collectionDiagnostic testsChain reaction
2021
Increased Detection of Mycobacterium tuberculosis Disease Using a Tissue-Based Laboratory-Developed Polymerase Chain Reaction Assay Compared to Standard Diagnostics.
Mackow NA, Abi-Raad R, Kerantzas CA, Hui P, Malinis M, Azar MM. Increased Detection of Mycobacterium tuberculosis Disease Using a Tissue-Based Laboratory-Developed Polymerase Chain Reaction Assay Compared to Standard Diagnostics. American Journal Of Tropical Medicine And Hygiene 2021, 105: 1657-1661. PMID: 34544041, PMCID: PMC8641361, DOI: 10.4269/ajtmh.21-0104.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedCulture TechniquesFemaleHumansLungLymph NodesMaleMiddle AgedMycobacterium tuberculosisPleuraReal-Time Polymerase Chain ReactionReference StandardsRetrospective StudiesSensitivity and SpecificitySputumTuberculosisTuberculosis, Lymph NodeTuberculosis, Multidrug-ResistantTuberculosis, PleuralTuberculosis, PulmonaryConceptsComposite reference standardMTB PCRAFB cultureMycobacterium tuberculosisPolymerase chain reactionAcid-fast bacilli smearMycobacterium tuberculosis diseasePositive AFB cultureChain reactionReal-time polymerase chain reactionStandard diagnosticsBacilli smearMTB casesTuberculosis diseaseClinical sensitivityLong turnaround timeXpertClinical performanceReference standardPCRVariable sensitivityTurnaround timeLymphPatientsTuberculosis
2020
Does Specimen Type Have an Impact on HER2 Status in Endometrial Serous Carcinoma? Discordant HER2 Status of Paired Endometrial Biopsy and Hysterectomy Specimens in the Presence of Frequent Intratumoral Heterogeneity
Rottmann D, Assem H, Matsumoto N, Wong S, Hui P, Buza N. Does Specimen Type Have an Impact on HER2 Status in Endometrial Serous Carcinoma? Discordant HER2 Status of Paired Endometrial Biopsy and Hysterectomy Specimens in the Presence of Frequent Intratumoral Heterogeneity. International Journal Of Gynecological Pathology 2020, 40: 263-271. PMID: 32897955, DOI: 10.1097/pgp.0000000000000690.Peer-Reviewed Original ResearchMeSH KeywordsAgedAged, 80 and overAlgorithmsBiomarkers, TumorBiopsyEndometrial NeoplasmsEndometriumFemaleHumansHysterectomyImmunohistochemistryMiddle AgedReceptor, ErbB-2Sensitivity and SpecificityConceptsEndometrial serous carcinomaDiscordant HER2 statusHER2 immunohistochemical scoresHER2 protein expressionSerous carcinomaHER2 statusEndometrial biopsies/curettingsImmunohistochemical scoreProtein expressionHER2 testingIntratumoral heterogeneityEndometrial biopsy/curettageProlonged progression-free survivalHuman epidermal growth factor receptor 2Epidermal growth factor receptor 2HER2 testing algorithmProgression-free survivalGrowth factor receptor 2HER2-negative tumorsFinal study cohortHER2-positive tumorsRecent clinical trialsSpecimen typesFactor receptor 2Optimal specimen types
2015
Grading of atypia in genital skin lesions: routine microscopic evaluation and use of p16 immunostaining
Ezaldein H, Lott JP, McNiff JM, Hui P, Buza N, Ko CJ. Grading of atypia in genital skin lesions: routine microscopic evaluation and use of p16 immunostaining. Journal Of Cutaneous Pathology 2015, 42: 519-526. PMID: 25951050, DOI: 10.1111/cup.12525.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overBiomarkers, TumorCross-Sectional StudiesCyclin-Dependent Kinase Inhibitor p16FemaleGenitalia, FemaleHumansImmunohistochemistryMaleMiddle AgedNeoplasm ProteinsObserver VariationPapillomavirus InfectionsSensitivity and SpecificitySkin DiseasesSquamous Intraepithelial Lesions of the CervixUterine Cervical NeoplasmsConceptsGenital skin lesionsHigh-grade squamous intraepithelial lesionsLow-grade squamous intraepithelial lesionsSquamous intraepithelial lesionsP16 immunostainingSkin lesionsDiagnostic agreementIntraepithelial lesionsUseful adjunctive markerHigh-grade dysplasiaCross-sectional studyRoutine microscopic evaluationDegree of atypiaAdjunctive markerInter-observer agreementPrimary outcomeCervical lesionsHistopathologic assessmentHistopathologic evaluationConsensus diagnosisReactive atypiaEosin stainingOriginal diagnosisAtypiaLesions
2012
Comparing Two Methods of Detection for Chlamydia trachomatis in Liquid-Based Papanicolaou Tests
Levi AW, Beckman D, Hui P, Schofield K, Harigopal M, Chhieng DC. Comparing Two Methods of Detection for Chlamydia trachomatis in Liquid-Based Papanicolaou Tests. American Journal Of Clinical Pathology 2012, 138: 236-240. PMID: 22904135, DOI: 10.1309/ajcp2b7xqtcnamjp.Peer-Reviewed Original ResearchConceptsHC2 assayBD Viper SystemXTR TechnologyPositive predictive valueDNA assaysLiquid-based Papanicolaou testsNucleic acidsC trachomatisChlamydia trachomatisPredictive valueHybrid Capture 2Reverse transcriptase-polymerase chain reactionChlamydia trachomatis testingTranscriptase-polymerase chain reactionViper SystemComparable specificityCapture 2Papanicolaou testChlamydial infectionCytology samplesMethod of detectionThinPrep specimensTrachomatisAssaysSensitivity rateDiagnostic value of K‐ras mutation analysis for pancreaticobiliary cytology specimens with indeterminate diagnosis
Cai G, Mahooti S, Lipata FM, Chhieng D, Hui P. Diagnostic value of K‐ras mutation analysis for pancreaticobiliary cytology specimens with indeterminate diagnosis. Cancer Cytopathology 2012, 120: 313-318. PMID: 22367918, DOI: 10.1002/cncy.21188.Peer-Reviewed Original Research
2011
Use of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions
Levi AW, Harigopal M, Hui P, Schofield K, Chhieng DC. Use of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions. Cancer Cytopathology 2011, 119: 228-234. PMID: 21732551, DOI: 10.1002/cncy.20172.Peer-Reviewed Original ResearchConceptsLow-grade squamous intraepithelial lesionsHR-HPV testingHPV testingCIN 2Colposcopy rateHR-HPVRelative light units/cutoffCervical intraepithelial neoplasia 2High-risk human papillomavirusHR-HPV statusRLU/COIntraepithelial neoplasia 2Hybrid Capture 2Squamous intraepithelial lesionsCIN 1 lesionsLarge teaching hospitalNegative predictive valueLSIL cytologyHPV statusIntraepithelial lesionsCapture 2Human papillomavirusTeaching hospitalCytologic diagnosisSevere lesions
2010
Molecular diagnosis of gestational trophoblastic disease
Hui P. Molecular diagnosis of gestational trophoblastic disease. Expert Review Of Molecular Diagnostics 2010, 10: 1023-1034. PMID: 21080819, DOI: 10.1586/erm.10.93.Peer-Reviewed Original Research
2003
Real-time Quantitative RT-PCR of Cyclin D1 mRNA in Mantle Cell Lymphoma: Comparison with FISH and Immunohistochemistry
Hui* P, Howe* J, Crouch J, Nimmakayalu M, Qumsiyeh MB, Tallini G, Flynn SD, Smith BR. Real-time Quantitative RT-PCR of Cyclin D1 mRNA in Mantle Cell Lymphoma: Comparison with FISH and Immunohistochemistry. Leukemia & Lymphoma 2003, 44: 1385-1394. PMID: 12952233, DOI: 10.1080/1042819031000079168.Peer-Reviewed Original ResearchCyclin D1Diagnosis, DifferentialFalse Negative ReactionsHumansImmunohistochemistryIn Situ Hybridization, FluorescenceLymphoma, B-CellLymphoma, Mantle-CellReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, NeoplasmSensitivity and SpecificityTranslocation, Genetic