1998
Elucidation of the genetic basis of the antigen presentation defects in the mutant cell line .220 reveals polymorphism and alternative splicing of the tapasin gene
Copeman J, Bangia N, Cross J, Cresswell P. Elucidation of the genetic basis of the antigen presentation defects in the mutant cell line .220 reveals polymorphism and alternative splicing of the tapasin gene. European Journal Of Immunology 1998, 28: 3783-3791. PMID: 9842921, DOI: 10.1002/(sici)1521-4141(199811)28:11<3783::aid-immu3783>3.0.co;2-9.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAntigen PresentationAntiportersATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersB-LymphocytesCell LineDNA, ComplementaryEndoplasmic ReticulumExonsHumansImmunoglobulinsMembrane Transport ProteinsMutationPolymorphism, GeneticReverse Transcriptase Polymerase Chain ReactionConceptsMutant cell linesEndoplasmic reticulumAlternative splicingN-terminal 49 amino acidsGenetic basisTapasin geneExon twoWild-type cellsFull-length transcriptsCell linesSingle nucleotide substitutionSignal peptideSecond intronNucleotide substitutionsPhysical associationSplice siteGlycoprotein tapasinPosition 240Amino acidsClass I moleculesSplicingOptimal bindingGenesI moleculesHeterodimersSoluble Tapasin Restores MHC Class I Expression and Function in the Tapasin-Negative Cell Line .220
Lehner P, Surman M, Cresswell P. Soluble Tapasin Restores MHC Class I Expression and Function in the Tapasin-Negative Cell Line .220. Immunity 1998, 8: 221-231. PMID: 9492003, DOI: 10.1016/s1074-7613(00)80474-4.Peer-Reviewed Original ResearchAntigen PresentationAntiportersATP Binding Cassette Transporter, Subfamily B, Member 2ATP Binding Cassette Transporter, Subfamily B, Member 3ATP-Binding Cassette TransportersBiological TransportEndoplasmic ReticulumHLA-B8 AntigenHumansImmunoglobulinsMembrane Transport ProteinsMutationPeptidesProtein BindingSolubilityT-Lymphocytes, Cytotoxic
1996
Roles for Calreticulin and a Novel Glycoprotein, Tapasin, in the Interaction of MHC Class I Molecules with TAP
Sadasivan B, Lehner P, Ortmann B, Spies T, Cresswell P. Roles for Calreticulin and a Novel Glycoprotein, Tapasin, in the Interaction of MHC Class I Molecules with TAP. Immunity 1996, 5: 103-114. PMID: 8769474, DOI: 10.1016/s1074-7613(00)80487-2.Peer-Reviewed Original ResearchATP Binding Cassette Transporter, Subfamily B, Member 2ATP-Binding Cassette TransportersBeta 2-MicroglobulinCalcium-Binding ProteinsCalreticulinCell LineGlycoproteinsHistocompatibility Antigens Class IHLA-A AntigensHLA-B AntigensHLA-C AntigensHumansIndolizinesMolecular ChaperonesMutationPeptidesRibonucleoproteins
1994
In vivo and in vitro formation and dissociation of HLA-DR complexes with invariant chain-derived peptides
Avva R, Cresswell P. In vivo and in vitro formation and dissociation of HLA-DR complexes with invariant chain-derived peptides. Immunity 1994, 1: 763-774. PMID: 7895165, DOI: 10.1016/s1074-7613(94)80018-9.Peer-Reviewed Original ResearchConceptsHLA-DM expressionDR alpha beta dimersHLA-DR moleculesCLIP dissociationClass II moleculesHLA-DR complexInvariant chain peptideII-peptide complexesPrimary aliphatic aminesResidual fragmentsChain-derived peptideClass IIHLA-DMAliphatic aminesCarboxylic acidsSuch complexesUnbranched hydrocarbonsAlpha beta dimersChain peptideInvariant chain-derived peptidesComplexesVivoCLIP complexBeta dimersDissociationAssembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells
Denzin L, Robbins N, Carboy-Newcomb C, Cresswell P. Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells. Immunity 1994, 1: 595-606. PMID: 7600288, DOI: 10.1016/1074-7613(94)90049-3.Peer-Reviewed Original ResearchAntigen PresentationAntigens, Differentiation, B-LymphocyteBase SequenceBiological TransportCell LineCell MembraneFlow CytometryHistocompatibility Antigens Class IIHLA-D AntigensHLA-DR3 AntigenHumansHybrid CellsKineticsMolecular Sequence DataMutationNeoplasm ProteinsPrecipitin TestsSodium Dodecyl SulfateTime FactorsTransfectionAntigen Presentation: Getting peptides into MHC class II molecules
Cresswell P. Antigen Presentation: Getting peptides into MHC class II molecules. Current Biology 1994, 4: 541-543. PMID: 7922377, DOI: 10.1016/s0960-9822(00)00119-6.Peer-Reviewed Original Research
1992
HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides
Riberdy J, Newcomb J, Surman M, Barbosat J, Cresswell P. HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides. Nature 1992, 360: 474-477. PMID: 1448172, DOI: 10.1038/360474a0.Peer-Reviewed Original ResearchConceptsClass II moleculesInvariant chainMajor histocompatibility complex (MHC) class II moleculesCell linesHLA-DR moleculesClass II binding siteInvariant chain peptideHLA-DR3 moleculesMore MHCClass IIAntigen processingT2 transfectantsAntigenic peptidesHuman cell linesChain peptideVivo conditionsPeptidesLarge proportionProteolytic cleavageMutant cell linesCertain human cell linesEarly stagesTransfectantsHLA-A2 molecules in an antigen-processing mutant cell contain signal sequence-derived peptides
Wei M, Cresswell P. HLA-A2 molecules in an antigen-processing mutant cell contain signal sequence-derived peptides. Nature 1992, 356: 443-446. PMID: 1557127, DOI: 10.1038/356443a0.Peer-Reviewed Original Research
1980
HLA-A2 as a target for cell-mediated lympholysis: Evidence from immunoselected HLA-A2 negative mutant cell lines
Andreotti P, Apgar J, Cresswell P. HLA-A2 as a target for cell-mediated lympholysis: Evidence from immunoselected HLA-A2 negative mutant cell lines. Human Immunology 1980, 1: 77-86. PMID: 7263311, DOI: 10.1016/0198-8859(80)90011-7.Peer-Reviewed Original ResearchConceptsCytotoxic T lymphocytesCell-mediated lympholysisHLA-A2T lymphocytesMixed lymphocyte responseHLA-A2 mutantsCell linesLymphocyte responsesHLA typingImmune precipitation analysisLymphocytesTarget cellsMolecular targetsSensitized cellsMutant cell linesComparable susceptibilityLympholysisHLAStaphylococcus aureus proteinPutative targetsAureus proteinNegative selectionCellsTargetSusceptibility