1996
Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice
Leach E, Narayanan L, Havre P, Gunther E, Yeasky T, Glazer P. Other transgenic mutation assays: Tissue specificity of spontaneous point mutations in λsupF transgenic mice. Environmental And Molecular Mutagenesis 1996, 28: 459-464. PMID: 8991078, DOI: 10.1002/(sici)1098-2280(1996)28:4<459::aid-em23>3.0.co;2-d.Peer-Reviewed Original Research
1995
Induction of p53 in mouse cells decreases mutagenesis by UV radiation
Yuan J, Yeasky T, Havre P, Glazer P. Induction of p53 in mouse cells decreases mutagenesis by UV radiation. Carcinogenesis 1995, 16: 2295-2300. PMID: 7586125, DOI: 10.1093/carcin/16.10.2295.Peer-Reviewed Original ResearchConceptsInduction of p53Cell cycle blockCell linesCycle blockRole of p53Cell cycle analysisInvolvement of p53Lambda phage shuttle vectorWestern blotChromosomal damageClonogenic survivalNucleotide excision repairUV-induced mutationsCellular DNA damageP53 alleleRecent evidenceP53Recoverable lambda phage shuttle vectorFibroblast cell lineMutation reporter geneUV-induced lesionsG1 phaseP53 activityMouse fibroblast cell lineReporter geneMutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links.
Gunther E, Yeasky T, Gasparro F, Glazer P. Mutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links. Cancer Research 1995, 55: 1283-8. PMID: 7882323.Peer-Reviewed Original ResearchConceptsLambda phage shuttle vectorMutation reporter geneMammalian cellsCross-linkable sitesFibroblast cell lineMouse fibroblast cell lineReporter geneMolecular eventsShuttle vectorSpectrum of mutationsMouse fibroblastsPremutagenic lesionsSupF geneCross-link formationMutationsC transversionCell linesMutagenesisGenesApt sitePsoralen treatmentCellsSitesDNAFrequent T:A→G:C transversions in X-irradiated mouse cells
Yuan J, Yeasky T, Rhee M, Glazer P. Frequent T:A→G:C transversions in X-irradiated mouse cells. Carcinogenesis 1995, 16: 83-88. PMID: 7834808, DOI: 10.1093/carcin/16.1.83.Peer-Reviewed Original ResearchConceptsMammalian cellsMouse cellsC transversionRadiation-induced point mutationsLambda phage shuttle vectorPoint mutationsMutation reporter geneRecoverable lambda phage shuttle vectorDistinctive mutational signaturesUnique mutational spectrumPattern of mutationsReporter geneSpontaneous mutationsShuttle vectorCellular repair processesMultiple copiesMutational signaturesPolymerase betaMouse fibroblastsMutationsMutational spectrumTransversionsRepair processCellsPossible role
1989
Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice
Summers W, Glazer P, Malkevich D. Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice. Mutation Research/Fundamental And Molecular Mechanisms Of Mutagenesis 1989, 220: 263-268. PMID: 2522589, DOI: 10.1016/0165-1110(89)90030-4.Peer-Reviewed Original ResearchConceptsMammalian DNALambda phage shuttle vectorForeign DNA sequencesLambda packaging extractsAnalysis of mutationsInfectious phage particlesMammalian cellsBacteriophage genomesGene sequencesDNA sequencesChromosomal sequencesBacterial systemsMammalian mutagenesisShuttle vectorLambda phageMolecular levelPhage particlesE. coliDNATransgenic miceSequenceMutationsGenomeMutagenesisPhages
1986
Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.
Glazer P, Sarkar S, Summers W. Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1041-1044. PMID: 2937054, PMCID: PMC323006, DOI: 10.1073/pnas.83.4.1041.Peer-Reviewed Original ResearchConceptsMammalian cellsStable mouse L cell lineLambda phage shuttle vectorMouse cell DNACell DNAMammalian cell DNASupF geneMouse L cell lineDNA sequence analysisLambda phage vectorL cell lineMutant phageMouse cellsSequence analysisShuttle vectorUV-induced mutationsMultiple copiesUV mutagenesisPhage vectorEscherichia coliMutagenesisViable phagePhagesGenesT transition