1993
Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas
Wikstrand C, Stanley S, Humphrey P, Pegram C, Archer G, Kurpad S, Shibuya M, Bigner D. Investigation of a synthetic peptide as immunogen for a variant epidermal growth factor receptor associated with gliomas. Journal Of Neuroimmunology 1993, 46: 165-173. PMID: 8360327, DOI: 10.1016/0165-5728(93)90246-u.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorGrowth factor receptorVariant receptorHuman epidermal growth factor receptorFactor receptorVariant epidermal growth factor receptorResponse of miceHuman tumor samplesAdditional immunizationsPeptide immunizationAntibody activityAntibody productionSynthetic peptidesTumor samplesImmunizationReceptorsRabbitsSingle goatGliomasTitersReceptor proteinVariant formImmunogenPeptides
1992
Monoclonal antibodies to malignant human gliomas
Wikstrand C, Fredman P, Svennerholm L, Humphrey P, Bigner S, Bigner D. Monoclonal antibodies to malignant human gliomas. Journal Of Molecular Neuroscience 1992, 17: 137-146. PMID: 1384525, DOI: 10.1007/bf03159988.Peer-Reviewed Original ResearchConceptsPrimary CNS neoplasmsCNS neoplasmsHuman gliomasMonoclonal antibodiesMalignant brain tumorsHuman glioma xenograftsMalignant human gliomasAdult human brainNormal adult tissuesMAb localizationSpecific monoclonal antibodiesGlioma xenograftsBrain tumorsCytologic specimensFrozen sectionsEpitopic specificityGlioma biopsiesEGFR geneNeoplasmsGliomasVivo diagnosisMutant EGFREGFR proteinAntibodiesTumors
1991
Deletion-mutant epidermal growth factor receptor in human gliomas: Effect of type II mutation on receptor function
Humphrey P, Gangarosa L, Wong A, Archer G, Lund-Johansen M, Bjerkvig R, Laerum O, Friedman H, Bigner D. Deletion-mutant epidermal growth factor receptor in human gliomas: Effect of type II mutation on receptor function. Biochemical And Biophysical Research Communications 1991, 178: 1413-1420. PMID: 1678600, DOI: 10.1016/0006-291x(91)91051-d.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceCell LineChromosome DeletionEpidermal Growth FactorErbB ReceptorsGene AmplificationGene RearrangementGliomaHumansKineticsMolecular Sequence DataPhosphorylationProtein-Tyrosine KinasesProto-Oncogene MasProto-Oncogene ProteinsProto-OncogenesReceptor, ErbB-2Transforming Growth Factor alphaConceptsEGFR kinase activityMutant EGFRDeletion-mutant epidermal growth factor receptorGrowth factor receptor geneMutant EGFR proteinEpidermal growth factor receptorGrowth factorFactor receptor geneEpidermal growth factor receptor (EGFR) geneGrowth factor receptorKinase activityExtracellular domainGlioma cell proliferationDomain IVFrame deletionType II mutationAmino acidsEGFR proteinFactor receptorReceptor geneThree-dimensional spheroid modelCell proliferationReceptor functionEGFHuman gliomas
1988
Comparative localization of murine monoclonal antibody Me1-14 F(ab')2 fragment and whole IgG2a in human glioma xenografts.
Colapinto E, Humphrey P, Zalutsky M, Groothuis D, Friedman H, de Tribolet N, Carrel S, Bigner D. Comparative localization of murine monoclonal antibody Me1-14 F(ab')2 fragment and whole IgG2a in human glioma xenografts. Cancer Research 1988, 48: 5701-7. PMID: 3167830.Peer-Reviewed Original ResearchConceptsHuman glioma cell line DNormal tissuesSpecific tumor localizationHuman glioma xenograftsXenograft-bearing miceNormal tissue ratiosHigh radiation dosesImmunoglobulin G2aAthymic miceGlioma xenograftsBrain tumorsBrain tumor diagnosisTumor localizationHigh tumorTumor uptakeIntracranial xenograftsComparative localizationTherapeutic agentsMonoclonal antibodiesMAb fragmentsTissue ratioTumor diagnosisPotential agentRadiation dosesSelective deliveryThe Localisation of Radiolabeled Murine Monoclonal Antibody 81C6 and its Fab Fragment in Human Glioma Xenografts in Athymic Mice
Colapinto E, Lee Y, Humphrey P, Zalutsky M, Friedman H, Bullard D, Bigner D. The Localisation of Radiolabeled Murine Monoclonal Antibody 81C6 and its Fab Fragment in Human Glioma Xenografts in Athymic Mice. British Journal Of Neurosurgery 1988, 2: 179-191. PMID: 3267302, DOI: 10.3109/02688698808992668.Peer-Reviewed Original ResearchConceptsMab 81C6Monoclonal antibody 81C6Dose/Athymic miceFab fragmentsHuman glioma xenograftsMG glioma cellsGlioma xenograftsHigh immunoreactivityIntracranial xenograftsGlioma cellsExtracellular matrix antigensNormal tissuesTissue ratioMarked lossRadiation dosesXenograftsImmunoreactivityTumorsMatrix antigensAntigenMiceTheoretical benefitsFab levelsGliomas