1999
Controlled Conformational Transitions in the MVM Virion Expose the VP1 N-Terminus and Viral Genome without Particle Disassembly
Cotmore S, D'Abramo A, Ticknor C, Tattersall P. Controlled Conformational Transitions in the MVM Virion Expose the VP1 N-Terminus and Viral Genome without Particle Disassembly. Virology 1999, 254: 169-181. PMID: 9927584, DOI: 10.1006/viro.1998.9520.Peer-Reviewed Original ResearchConceptsViral particlesPrior treatmentAntigenic determinantsCapsid protein VP1Empty viral particlesParvovirus minute virusBrief exposureInfectious virionsIndividual polypeptide speciesSerumProtein VP1Proportion of virionsVirusVirionsEpitope accessibilityMinute virusApparent integrityViral genomeGreat majorityVP1ProportionMice
1995
Sequence Motifs in the Replicator Protein of Parvovirus MVM Essential for Nicking and Covalent Attachment to the Viral Origin: Identification of the Linking Tyrosine
Nüesch J, Cotmore S, Tattersall P. Sequence Motifs in the Replicator Protein of Parvovirus MVM Essential for Nicking and Covalent Attachment to the Viral Origin: Identification of the Linking Tyrosine. Virology 1995, 209: 122-135. PMID: 7747462, DOI: 10.1006/viro.1995.1236.Peer-Reviewed Original ResearchConceptsMutant proteinsRolling-circle replicationTyrosine motifOrigin-containing plasmidParvoviral DNA replicationViral originParvovirus minute virusSingle-strand nicksInitiator proteinSequence motifsDNA replicationSite-specific bindingSequence comparisonCyanogen bromide cleavageOrigin sequencesDe novo synthesisSubstrate DNAY210Circle replicationLatter residueStrand nicksHeLa cellsLow salt conditionsCommon motifMetal coordination sites
1993
Nuclear Targeting of the Parvoviral Replicator Molecule NS1: Evidence for Self-Association Prior to Nuclear Transport
Nüesch J, Tattersall P. Nuclear Targeting of the Parvoviral Replicator Molecule NS1: Evidence for Self-Association Prior to Nuclear Transport. Virology 1993, 196: 637-651. PMID: 8372437, DOI: 10.1006/viro.1993.1520.Peer-Reviewed Original ResearchConceptsNuclear localizationMutant NS1 proteinsNS1 polypeptideMutant NS1Nuclear localization signalNS1 proteinMajor non-structural proteinsAmino acid substitutionsParvovirus minute virusNon-structural proteinsLocalization signalPutative NTPNuclear transportLysine motifNuclear targetingMammalian cellsSubstitution mutantsExpression systemAcid substitutionsFrame deletionDouble lysineProteinMinute virusSubcellular fractionsViral DNA
1992
Two amino acid substitutions within the capsid are coordinately required for acquisition of fibrotropism by the lymphotropic strain of minute virus of mice
Ball-Goodrich L, Tattersall P. Two amino acid substitutions within the capsid are coordinately required for acquisition of fibrotropism by the lymphotropic strain of minute virus of mice. Journal Of Virology 1992, 66: 3415-3423. PMID: 1316457, PMCID: PMC241122, DOI: 10.1128/jvi.66.6.3415-3423.1992.Peer-Reviewed Original ResearchConceptsRestrictive infectionDouble mutant virusLymphotropic strainViral life cycleMinute virusInfectionImmunosuppressive strainFibroblast infectionVirusParvovirus minute virusMutant virusA9 fibroblastsCodon 317MiceCodon 321Amino acid substitutionsFibroblastsSame cellsCapsid geneCellsGene expressionAcid substitutionsSynergistic interactionNucleotide changesA9 cells
1989
A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles
Cotmore S, Tattersall P. A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles. Journal Of Virology 1989, 63: 3902-3911. PMID: 2527311, PMCID: PMC250986, DOI: 10.1128/jvi.63.9.3902-3911.1989.Peer-Reviewed Original ResearchConceptsNS-1 moleculesHost cellsSingle-strand DNA genomeNonstructural protein NS-1Amino-terminal domainNew host cellsNS-1 polypeptideAutonomous parvovirus minute virusParvovirus minute virusNS-1Normal culture conditionsDNA genomeS2 formA9 cellsLimited proteolysisIncoming virusMinute virusProteolytic digestionDNADNA coreMost virionsCulture conditionsVirionsPolypeptideParvovirus particlesEvidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice
Cotmore S, Gunther M, Tattersall P. Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice. Journal Of Virology 1989, 63: 1002-1006. PMID: 2911112, PMCID: PMC247784, DOI: 10.1128/jvi.63.2.1002-1006.1989.Peer-Reviewed Original Research
1988
Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice
Gardiner E, Tattersall P. Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice. Journal Of Virology 1988, 62: 2605-2613. PMID: 3392768, PMCID: PMC253690, DOI: 10.1128/jvi.62.8.2605-2613.1988.Peer-Reviewed Original ResearchConceptsFibroblast host cellsLymphotropic strainHost cell typesLymphocyte culturesMinute virusPlaque formationTarget cell specificityChimeric viral genomesFibroblast monolayersViral growthParvovirus minute virusViral progenyVirusHost range determinantsMiceHost cellsCell specificityCell typesRecombinant virusesViral sequencesViral genomeViral promotersInfectious genomic cloneVirus structural genesRange determinantsEvidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated
Gardiner E, Tattersall P. Evidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated. Journal Of Virology 1988, 62: 1713-1722. PMID: 3357208, PMCID: PMC253210, DOI: 10.1128/jvi.62.5.1713-1722.1988.Peer-Reviewed Original ResearchConceptsProgeny virusIncoming viral capsidInfectious molecular cloneViral transcriptionInfectious centersAllotropic determinantHost cell susceptibilityImmunosuppressive strainAutonomous parvovirus minute virusMolecular cloneCell susceptibilityViral RNASame low levelVirusParvovirus minute virusCell linesRescue of virusPhenotype characteristicViral DNAIntracellular factorsDifferent infectivitySimilar numberFibroblastsMinute virusMouse cell lines
1986
Organization of nonstructural genes of the autonomous parvovirus minute virus of mice
Cotmore S, Tattersall P. Organization of nonstructural genes of the autonomous parvovirus minute virus of mice. Journal Of Virology 1986, 58: 724-732. PMID: 2939261, PMCID: PMC252977, DOI: 10.1128/jvi.58.3.724-732.1986.Peer-Reviewed Original ResearchConceptsOpen reading frameAutonomous parvovirus minute virusParvovirus minute virusSimilar proteinsFusion proteinCommon amino-terminal sequenceSingle open reading frameNonstructural protein NS-1Rabbit reticulocyte lysate translation systemViral genomeReticulocyte lysate translation systemAlternative open reading framesNS-2 proteinsCarboxy-terminal halfNS-1 proteinNS-1Amino acid sequenceBacterial fusion proteinLysate translation systemMinute virusAmino-terminal sequenceProcaryotic expression vectorR2 transcriptsReading frameAcid sequence
1983
Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice
Merchlinsky M, Tattersall P, Leary J, Cotmore S, Gardiner E, Ward D. Construction of an infectious molecular clone of the autonomous parvovirus minute virus of mice. Journal Of Virology 1983, 47: 227-232. PMID: 6345805, PMCID: PMC255236, DOI: 10.1128/jvi.47.1.227-232.1983.Peer-Reviewed Original Research