Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments
Cotmore S, McKie V, Anderson L, Astell C, Tattersall P. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments. Journal Of Virology 1986, 60: 548-557. PMID: 3021988, PMCID: PMC288924, DOI: 10.1128/jvi.60.2.548-557.1986.Peer-Reviewed Original ResearchConceptsProtein sequencesViral capsid polypeptidesCapsid polypeptidesSucrose velocity gradientsB19 genomeBacterial plasmid vectorRestriction endonuclease fragmentsGenomic fragmentProtein speciesApparent molecular weightNonstructural polypeptidesExpression constructsSimilar proteinsBAL-31GenomeExpression vectorNonstructural proteinsEndonuclease fragmentsPlasmid vectorViral genomeViral polypeptidesPolypeptide fragmentsProcaryotic expressionPolypeptideProteinThe Role of Parvovirus B19 in Aplastic Crisis and Erythema Infectiosum (Fifth Disease)
Chorba T, Coccia P, Holman R, Tattersall P, Anderson L, Sudman J, Young N, Kurczynski E, Saarinen U, Moir R, Lawrence D, Jason J, Evatt B. The Role of Parvovirus B19 in Aplastic Crisis and Erythema Infectiosum (Fifth Disease). The Journal Of Infectious Diseases 1986, 154: 383-393. PMID: 3016109, DOI: 10.1093/infdis/154.3.383.Peer-Reviewed Original ResearchConceptsErythema infectiosumAplastic crisisParvovirus B19B19-specific IgMSecondary attack rateSimultaneous outbreaksThroat garglesB19 infectionClinical entityRecent infectionAsymptomatic infectionSusceptible contactsPatientsInfectionB19Attack rateSerumComparison groupIgMHousehold membersOutbreakGargleEpidemiologyUrineOrganization of nonstructural genes of the autonomous parvovirus minute virus of mice
Cotmore S, Tattersall P. Organization of nonstructural genes of the autonomous parvovirus minute virus of mice. Journal Of Virology 1986, 58: 724-732. PMID: 2939261, PMCID: PMC252977, DOI: 10.1128/jvi.58.3.724-732.1986.Peer-Reviewed Original ResearchConceptsOpen reading frameAutonomous parvovirus minute virusParvovirus minute virusSimilar proteinsFusion proteinCommon amino-terminal sequenceSingle open reading frameNonstructural protein NS-1Rabbit reticulocyte lysate translation systemViral genomeReticulocyte lysate translation systemAlternative open reading framesNS-2 proteinsCarboxy-terminal halfNS-1 proteinNS-1Amino acid sequenceBacterial fusion proteinLysate translation systemMinute virusAmino-terminal sequenceProcaryotic expression vectorR2 transcriptsReading frameAcid sequenceHuman parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia.
Saarinen U, Chorba T, Tattersall P, Young N, Anderson L, Palmer E, Coccia P. Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. Blood 1986, 67: 1411-7. PMID: 3008891, DOI: 10.1182/blood.v67.5.1411.bloodjournal6751411.Peer-Reviewed Original ResearchConceptsRed cell aplasiaAcute-phase seraHuman parvovirus B19Cell aplasiaHemolytic anemiaHereditary hemolytic anemiaParvovirus B19B19-specific IgMHemoglobin SC diseaseDays of presentationB19-specific IgGParvovirus B19 DNASickle cell anemiaHuman parvovirus B19 DNAErythroid colony formationAbdominal symptomsSevere reticulocytopeniaAcute onsetAdditional patientsProdromal symptomsSC diseaseB19 DNAViremic seraPatientsCell anemia