2014
Complementation for an essential ancillary non-structural protein function across parvovirus genera
Mihaylov IS, Cotmore SF, Tattersall P. Complementation for an essential ancillary non-structural protein function across parvovirus genera. Virology 2014, 468: 226-237. PMID: 25194919, PMCID: PMC4254310, DOI: 10.1016/j.virol.2014.07.043.Peer-Reviewed Original ResearchConceptsCell cycle progressionAncillary proteinsProtein functionDNA replicationReplication centersNP1 proteinPrimary sequenceFunctional overlapProtein NS2Cycle progressionGenus BocaparvovirusGenus ProtoparvovirusLate defectsNP1 inductionParvovirus genusVirion productionMinute virusSpecific defectsCell populationsUninfected cellsGenusCell viabilityProteinHuman bocavirus 1NP1 expression
1990
Alternate splicing in a parvoviral nonstructural gene links a common amino-terminal sequence to downstream domains which confer radically different localization and turnover characteristics
Cotmore S, Tattersall P. Alternate splicing in a parvoviral nonstructural gene links a common amino-terminal sequence to downstream domains which confer radically different localization and turnover characteristics. Virology 1990, 177: 477-487. PMID: 2142555, DOI: 10.1016/0042-6822(90)90512-p.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAphidicolinBase SequenceCapsidCell DivisionChromosome MappingDiterpenesFluorescent Antibody TechniqueGenes, ViralL CellsMiceMinute virus of miceMolecular Sequence DataMolecular WeightParvoviridaeRNA SplicingRNA, ViralSequence Homology, Nucleic AcidViral Core ProteinsViral Nonstructural ProteinsViral Structural ProteinsConceptsCommon amino-terminal domainAmino-terminal domainNS-1 moleculesCommon amino-terminal sequenceNS-1 polypeptideAmino-terminal sequenceSodium dodecyl sulfate gel electrophoresisNS-1Dodecyl sulfate gel electrophoresisUnphosphorylated formInternal exonsAlternate splicingGene productsSulfate gel electrophoresisA9 cellsNonstructural genesSpliced formsPhosphorylated formDownstream domainContiguous sequencesNonstructural proteinsSpecies migratePeptide-specific antibodiesMinute virusTurnover characteristicsSusceptibility of human cells to killing by the parvoviruses H-1 and minute virus of mice correlates with viral transcription
Cornelis J, Chen Y, Spruyt N, Duponchel N, Cotmore S, Tattersall P, Rommelaere J. Susceptibility of human cells to killing by the parvoviruses H-1 and minute virus of mice correlates with viral transcription. Journal Of Virology 1990, 64: 2537-2544. PMID: 2139892, PMCID: PMC249429, DOI: 10.1128/jvi.64.6.2537-2544.1990.Peer-Reviewed Original ResearchConceptsViral mRNAsHuman cellsLevel of transcriptionMinute virusMajor viral transcriptViral DNA amplificationNonstructural polypeptidesGene productsOncogenic transformationGene expressionIntracellular localizationNonstructural proteinsViral transcriptionViral transcriptsTranscriptionViral genomeParvovirus HCell susceptibilityHuman fibroblastsVirus uptakeEpithelial cellsDNA amplificationResistant derivativesKeratinocyte lineDifferential susceptibility
1989
A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles
Cotmore S, Tattersall P. A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles. Journal Of Virology 1989, 63: 3902-3911. PMID: 2527311, PMCID: PMC250986, DOI: 10.1128/jvi.63.9.3902-3911.1989.Peer-Reviewed Original ResearchConceptsNS-1 moleculesHost cellsSingle-strand DNA genomeNonstructural protein NS-1Amino-terminal domainNew host cellsNS-1 polypeptideAutonomous parvovirus minute virusParvovirus minute virusNS-1Normal culture conditionsDNA genomeS2 formA9 cellsLimited proteolysisIncoming virusMinute virusProteolytic digestionDNADNA coreMost virionsCulture conditionsVirionsPolypeptideParvovirus particlesLimitations to the expression of parvoviral nonstructural proteins may determine the extent of sensitization of EJ-ras-transformed rat cells to minute virus of mice
Van Hille B, Duponchel N, Salomé N, Spruyt N, Cotmore S, Tattersall P, Cornelis J, Rommelaere J. Limitations to the expression of parvoviral nonstructural proteins may determine the extent of sensitization of EJ-ras-transformed rat cells to minute virus of mice. Virology 1989, 171: 89-97. PMID: 2525841, DOI: 10.1016/0042-6822(89)90514-x.Peer-Reviewed Original ResearchConceptsNonstructural proteinsNonstructural protein NS-1Parvoviral DNA replicationRat cellsParvoviral life cycleNonstructural viral proteinsDNA replicationRat cell linesSensitivity of cellsRas transformationP21ras proteinsNormal rat cellsGene expressionMVMp infectionParvovirus MVMpNRK cellsViral proteinsEarly blockProteinCell linesViral DNALife cycleNS-1MVMpExpressionEvidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice
Cotmore S, Gunther M, Tattersall P. Evidence for a ligation step in the DNA replication of the autonomous parvovirus minute virus of mice. Journal Of Virology 1989, 63: 1002-1006. PMID: 2911112, PMCID: PMC247784, DOI: 10.1128/jvi.63.2.1002-1006.1989.Peer-Reviewed Original Research
1988
The terminal protein of minute virus of mice is an 83 kilodalton polypeptide linked to specific forms of double‐stranded and single‐stranded viral DNA
Gunther M, Tattersall P. The terminal protein of minute virus of mice is an 83 kilodalton polypeptide linked to specific forms of double‐stranded and single‐stranded viral DNA. FEBS Letters 1988, 242: 22-26. PMID: 3203742, DOI: 10.1016/0014-5793(88)80977-3.Peer-Reviewed Original ResearchMapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice
Gardiner E, Tattersall P. Mapping of the fibrotropic and lymphotropic host range determinants of the parvovirus minute virus of mice. Journal Of Virology 1988, 62: 2605-2613. PMID: 3392768, PMCID: PMC253690, DOI: 10.1128/jvi.62.8.2605-2613.1988.Peer-Reviewed Original ResearchConceptsFibroblast host cellsLymphotropic strainHost cell typesLymphocyte culturesMinute virusPlaque formationTarget cell specificityChimeric viral genomesFibroblast monolayersViral growthParvovirus minute virusViral progenyVirusHost range determinantsMiceHost cellsCell specificityCell typesRecombinant virusesViral sequencesViral genomeViral promotersInfectious genomic cloneVirus structural genesRange determinantsEvidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated
Gardiner E, Tattersall P. Evidence that developmentally regulated control of gene expression by a parvoviral allotropic determinant is particle mediated. Journal Of Virology 1988, 62: 1713-1722. PMID: 3357208, PMCID: PMC253210, DOI: 10.1128/jvi.62.5.1713-1722.1988.Peer-Reviewed Original ResearchConceptsProgeny virusIncoming viral capsidInfectious molecular cloneViral transcriptionInfectious centersAllotropic determinantHost cell susceptibilityImmunosuppressive strainAutonomous parvovirus minute virusMolecular cloneCell susceptibilityViral RNASame low levelVirusParvovirus minute virusCell linesRescue of virusPhenotype characteristicViral DNAIntracellular factorsDifferent infectivitySimilar numberFibroblastsMinute virusMouse cell linesThe NS-1 polypeptide of minute virus of mice is covalently attached to the 5' termini of duplex replicative-form DNA and progeny single strands
Cotmore S, Tattersall P. The NS-1 polypeptide of minute virus of mice is covalently attached to the 5' termini of duplex replicative-form DNA and progeny single strands. Journal Of Virology 1988, 62: 851-860. PMID: 3339715, PMCID: PMC253642, DOI: 10.1128/jvi.62.3.851-860.1988.Peer-Reviewed Original Research
1987
Human Parvovirus B19 Infection During Pregnancy
Woernle C, Anderson L, Tattersall P, Davison J. Human Parvovirus B19 Infection During Pregnancy. The Journal Of Infectious Diseases 1987, 156: 17-20. PMID: 3036955, DOI: 10.1093/infdis/156.1.17.Peer-Reviewed Original ResearchConceptsIgM-positive womenB19 infectionFetal deathFifth diseaseIgM antibodies to B19Outbreak of fifth diseaseAssociated with fetal deathHuman parvovirus B19 infectionLow riskHigh riskAntibodies to B19Identified pregnant womenParvovirus B19 infectionHuman parvovirus B19No adverse effectsHydropic fetusesFetal infectionB19 DNACord serumParvovirus B19Pregnant womenPrenatal careHigh risk of exposureInfectionB19The Autonomously Replicating Parvoviruses of Vertebrates
Cotmore S, Tattersall P. The Autonomously Replicating Parvoviruses of Vertebrates. Advances In Virus Research 1987, 33: 91-174. PMID: 3296697, DOI: 10.1016/s0065-3527(08)60317-6.Peer-Reviewed Original ResearchConceptsHost cellsProductive replicationHost cell typesSpecific cell surface receptorsHost cell factorsCell surface receptorsDifferentiated stateAutonomous parvovirusesIntracellular interactionsCell typesCell cyclingSurface receptorsCellular levelHelper virusCell factorWhole animalParvovirus strainsReplicationViral particlesPathogenic processesVertebratesCellsVirusParvovirusParvovirus group
1986
Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments
Cotmore S, McKie V, Anderson L, Astell C, Tattersall P. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments. Journal Of Virology 1986, 60: 548-557. PMID: 3021988, PMCID: PMC288924, DOI: 10.1128/jvi.60.2.548-557.1986.Peer-Reviewed Original ResearchConceptsProtein sequencesViral capsid polypeptidesCapsid polypeptidesSucrose velocity gradientsB19 genomeBacterial plasmid vectorRestriction endonuclease fragmentsGenomic fragmentProtein speciesApparent molecular weightNonstructural polypeptidesExpression constructsSimilar proteinsBAL-31GenomeExpression vectorNonstructural proteinsEndonuclease fragmentsPlasmid vectorViral genomeViral polypeptidesPolypeptide fragmentsProcaryotic expressionPolypeptideProteinDetection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay
Anderson L, Tsou C, Parker R, Chorba T, Wulff H, Tattersall P, Mortimer P. Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay. Journal Of Clinical Microbiology 1986, 24: 522-526. PMID: 3021807, PMCID: PMC268963, DOI: 10.1128/jcm.24.4.522-526.1986.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayB19 IgG antibodiesYears of ageHuman parvovirus B19Aplastic crisisAntibody detection enzyme-linked immunosorbent assayB19 antibodiesIgG antibodiesParvovirus B19IgM enzyme-linked immunosorbent assayDays of onsetAntibody enzyme-linked immunosorbent assayAcute-phase seraDetection of antibodiesEnzyme-linked immunosorbentDNA hybridizationElectron microscopyB19 infectionB19 IgMFifth diseaseB19 DNAImmunoglobulin MSerum specimensLow titersHigh titersThe Role of Parvovirus B19 in Aplastic Crisis and Erythema Infectiosum (Fifth Disease)
Chorba T, Coccia P, Holman R, Tattersall P, Anderson L, Sudman J, Young N, Kurczynski E, Saarinen U, Moir R, Lawrence D, Jason J, Evatt B. The Role of Parvovirus B19 in Aplastic Crisis and Erythema Infectiosum (Fifth Disease). The Journal Of Infectious Diseases 1986, 154: 383-393. PMID: 3016109, DOI: 10.1093/infdis/154.3.383.Peer-Reviewed Original ResearchConceptsErythema infectiosumAplastic crisisParvovirus B19B19-specific IgMSecondary attack rateSimultaneous outbreaksThroat garglesB19 infectionClinical entityRecent infectionAsymptomatic infectionSusceptible contactsPatientsInfectionB19Attack rateSerumComparison groupIgMHousehold membersOutbreakGargleEpidemiologyUrineOrganization of nonstructural genes of the autonomous parvovirus minute virus of mice
Cotmore S, Tattersall P. Organization of nonstructural genes of the autonomous parvovirus minute virus of mice. Journal Of Virology 1986, 58: 724-732. PMID: 2939261, PMCID: PMC252977, DOI: 10.1128/jvi.58.3.724-732.1986.Peer-Reviewed Original ResearchConceptsOpen reading frameAutonomous parvovirus minute virusParvovirus minute virusSimilar proteinsFusion proteinCommon amino-terminal sequenceSingle open reading frameNonstructural protein NS-1Rabbit reticulocyte lysate translation systemViral genomeReticulocyte lysate translation systemAlternative open reading framesNS-2 proteinsCarboxy-terminal halfNS-1 proteinNS-1Amino acid sequenceBacterial fusion proteinLysate translation systemMinute virusAmino-terminal sequenceProcaryotic expression vectorR2 transcriptsReading frameAcid sequenceNucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis
Shade R, Blundell M, Cotmore S, Tattersall P, Astell C. Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis. Journal Of Virology 1986, 58: 921-936. PMID: 3701931, PMCID: PMC253001, DOI: 10.1128/jvi.58.3.921-936.1986.Peer-Reviewed Original ResearchConceptsNucleotide sequenceLarge open reading frameMajor nonstructural proteinOpen reading frameFull-length cloneMajor structural polypeptidesGenome organizationGenomic clonesPutative polypeptideTranscription unitEntire genomeReading frameDNA sequencesFourth promoterParvovirus genomeSequence informationNonstructural proteinsGenomeTerminal repeatDependovirus genusStructural polypeptidesViral DNAB19 genomePolypeptideSequenceThe NS-1 polypeptide of the autonomous parvovirus MVM is a nuclear phosphoprotein
Cotmore S, Tattersall P. The NS-1 polypeptide of the autonomous parvovirus MVM is a nuclear phosphoprotein. Virus Research 1986, 4: 243-250. PMID: 3739422, DOI: 10.1016/0168-1702(86)90003-1.Peer-Reviewed Original ResearchConceptsParvovirus MVMNS-1 proteinVitro translation productsMessenger RNA speciesNS-1 polypeptideSame primary sequencePeptide map analysisNon-structural proteinsRNA speciesNuclear phosphoproteinReplication complexTranslation productsKb transcriptPrimary sequenceVivo productNS-1SpeciesProteinPredominant formPhosphoproteinMVMTranscriptsPolypeptideNucleusMap analysisHuman parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia.
Saarinen U, Chorba T, Tattersall P, Young N, Anderson L, Palmer E, Coccia P. Human parvovirus B19-induced epidemic acute red cell aplasia in patients with hereditary hemolytic anemia. Blood 1986, 67: 1411-7. PMID: 3008891, DOI: 10.1182/blood.v67.5.1411.bloodjournal6751411.Peer-Reviewed Original ResearchConceptsRed cell aplasiaAcute-phase seraHuman parvovirus B19Cell aplasiaHemolytic anemiaHereditary hemolytic anemiaParvovirus B19B19-specific IgMHemoglobin SC diseaseDays of presentationB19-specific IgGParvovirus B19 DNASickle cell anemiaHuman parvovirus B19 DNAErythroid colony formationAbdominal symptomsSevere reticulocytopeniaAcute onsetAdditional patientsProdromal symptomsSC diseaseB19 DNAViremic seraPatientsCell anemiaDNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain
Astell C, Gardiner E, Tattersall P. DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain. Journal Of Virology 1986, 57: 656-669. PMID: 3502703, PMCID: PMC252781, DOI: 10.1128/jvi.57.2.656-669.1986.Peer-Reviewed Original Research