Human natural killer cells expressing the memory‐associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO− natural killer cells following stimulation with interleukin‐12
Fu X, Liu Y, Li L, Li Q, Qiao D, Wang H, Lao S, Fan Y, Wu C. Human natural killer cells expressing the memory‐associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO− natural killer cells following stimulation with interleukin‐12. Immunology 2011, 134: 41-49. PMID: 21711347, PMCID: PMC3173693, DOI: 10.1111/j.1365-2567.2011.03464.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAntigens, CDAntigens, Differentiation, T-LymphocyteCD56 AntigenCell CountCytotoxicity, ImmunologicFemaleGPI-Linked ProteinsGranzymesHumansInterferon-gammaInterleukin-12Interleukin-2 Receptor alpha SubunitK562 CellsKiller Cells, NaturalLectins, C-TypeLeukocyte Common AntigensLeukocytes, MononuclearLymphocyte ActivationLymphocyte SubsetsMaleMiddle AgedNK Cell Lectin-Like Receptor Subfamily CNK Cell Lectin-Like Receptor Subfamily KPleural EffusionReceptors, IgGReceptors, Interleukin-12Tuberculosis, PleuralYoung AdultConceptsPleural fluid cellsNatural killer cellsNK cellsKiller cellsInterleukin-12Human natural killer cellsAdaptive immune featuresCD45RO- NK cellsInnate immune lymphocytesHuman NK cellsIL-12 receptor β1Immune lymphocytesIL-12Tuberculous patientsTuberculous pleurisyImmune featuresGranzyme BMore interferonReceptor β1Mycobacterium tuberculosisCD45ROFluid cellsPresent studyCellsCD25Biological functions of Mycobacterium tuberculosis-specific CD4+T cells were impaired by tuberculosis pleural fluid
Li Q, Li L, Liu Y, Fu X, Wang H, Lao S, yang B, Wu C. Biological functions of Mycobacterium tuberculosis-specific CD4+T cells were impaired by tuberculosis pleural fluid. Immunology Letters 2011, 138: 113-121. PMID: 21549757, DOI: 10.1016/j.imlet.2011.03.008.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAmino Acid SequenceAntibodies, NeutralizingAntigens, BacterialBacterial ProteinsBody FluidsCD4-Positive T-LymphocytesCells, CulturedFemaleHumansInterferon-gammaInterleukin-10Interleukin-2LungLymphocyte ActivationMaleMiddle AgedMolecular Sequence DataMonocytesMycobacterium tuberculosisTransforming Growth Factor betaTuberculosis, PleuralTumor Necrosis Factor-alphaConceptsPleural fluid mononuclear cellsPleural fluidAntigen-specific cellular immune responsesFluid mononuclear cellsIFN-γ productionMycobacterium tuberculosis infectionCellular immune responsesProduction of IFNT cell functionDose-dependent mannerTuberculosis patientsIL-10Tuberculosis infectionTuberculosis pleurisyCytokine productionMononuclear cellsIL-2Activation moleculeImmune responseCell activation processCFSE labelingCell activationFunctional predominanceCell activityStrong inhibitory effect[Analysis of ESAT-6-specific multifunctional CD4+; T cells in pleural fluid from patients with tuberculous pleurisy].
Li L, Qiao D, Li Q, Zhang X, Lao S, Wu C. [Analysis of ESAT-6-specific multifunctional CD4+; T cells in pleural fluid from patients with tuberculous pleurisy]. Chinese Journal Of Cellular And Molecular Immunology 2011, 27: 26-8. PMID: 21208559.Peer-Reviewed Original ResearchConceptsRecombinant ESAT-6 proteinT cellsESAT-6 proteinCytokine productionMean fluorescence intensityPeptide poolsTB infectionIL-2Positive cellsTh1 cytokine productionSingle-positive cellsTriple-positive cellsTuberculous pleurisyESAT-6Cell levelSingle-cell levelFlow cytometrySimilar frequencyStimulationCytokinesIFNTNFInfectionBCGCells