2023
A pref-1-controlled non-inflammatory mechanism of insulin resistance
Huang Y, Cui D, Chen L, Tong H, Wu H, Muller G, Qi Y, Wang S, Xu J, Gao X, Fifield K, Wang L, Xia Z, Vanderluit J, Liu S, Leng L, Sun G, McGuire J, Young L, Bucala R, Qi D. A pref-1-controlled non-inflammatory mechanism of insulin resistance. IScience 2023, 26: 106923. PMID: 37283810, PMCID: PMC10239698, DOI: 10.1016/j.isci.2023.106923.Peer-Reviewed Original ResearchInsulin resistanceMIF secretionPref-1 expressionPref-1Fat-induced insulin resistancePlasma MIF levelsNon-inflammatory mechanismsActivation of PAR2Palmitic acid dietHigh fat-induced insulin resistanceWhite adipose tissueSubsequent insulin resistanceMIF levelsPAR2 expressionM2 macrophagesAcid dietAdipose tissueAMPK-dependent mannerEndothelial cellsSecretionIntegrin β1CellsExpressionHigh levelsInflammation
2021
CD74 is a regulator of hematopoietic stem cell maintenance
Becker-Herman S, Rozenberg M, Hillel-Karniel C, Gil-Yarom N, Kramer M, Barak A, Sever L, David K, Radomir L, Lewinsky H, Levi M, Friedlander G, Bucala R, Peled A, Shachar I. CD74 is a regulator of hematopoietic stem cell maintenance. PLOS Biology 2021, 19: e3001121. PMID: 33661886, PMCID: PMC7963458, DOI: 10.1371/journal.pbio.3001121.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntigens, Differentiation, B-LymphocyteBone Marrow CellsBone Marrow TransplantationCell LineageFemaleHealthy VolunteersHematopoietic Stem CellsHistocompatibility Antigens Class IIHumansIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMaleMiceMice, Inbred C57BLSignal TransductionConceptsMacrophage migration inhibitory factorHematopoietic stem cellsBone marrowCytokine macrophage migration inhibitory factorMigration inhibitory factorNumber of HSPCsTransplant protocolsCD18 expressionClinical transplantationInduced SurvivalCD74Inhibitory factorBM nicheCell surface receptorsSelf-renewal propertiesClinical insightsProgenitor cellsBlood cell lineagesSurface receptorsStem cellsHematopoietic stemCell lineagesCellsUndifferentiated cellsHematopoietic stem cell maintenance
2006
Immunosuppressive Factors in Cancer
Moss J, Metz C, Bucala R. Immunosuppressive Factors in Cancer. 2006 DOI: 10.1002/3527600906.mcb.200400129.Peer-Reviewed Original ResearchImmunosuppressive factorsTumor cellsHost's antitumor defenseHost immune responseHost immune systemSuccessful cancer therapeuticsCancer patientsAntitumor defenseImmune responseEffective treatmentEvasive mechanismsImmune systemCell surface receptorsCancer therapeuticsSurface receptorsCancerCellsPatientsCytokinesProstaglandinsAntigenMajor obstacleReceptors
2003
Chapter 45 Macrophage migration inhibitory factor (MIF)
Arenberg D, Bucala R. Chapter 45 Macrophage migration inhibitory factor (MIF). 2003, II: 1037-xxxiii. DOI: 10.1016/b978-012689663-3/50049-1.Peer-Reviewed Original ResearchMacrophage migration inhibitory factorMigration inhibitory factorRegulation of MIFCritical pro-inflammatory mediatorInhibitory factorPro-inflammatory mediatorsMIF receptorImmune cellsImmunosuppressive propertiesCellular proliferationWidespread expressionMediatorsFuture investigationsCellsInflammationCytokinesGlucocorticoidsPathogenesisSteroidsSecretionActivityImportant roleReceptors
2000
Macaque blood‐derived antigen‐presenting cells elicit SIV‐specific immune responses
Zhu Y, Koo K, Bradshaw J, Sutton W, Kuller L, Bucala R, Anderson D, Mossman S, Villinger F, Haigwood N. Macaque blood‐derived antigen‐presenting cells elicit SIV‐specific immune responses. Journal Of Medical Primatology 2000, 29: 182-192. PMID: 11085581, DOI: 10.1034/j.1600-0684.2000.290312.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationAntigen-Presenting CellsAntigens, CDAntigens, CD34Antigens, ViralCells, CulturedDendritic CellsGenes, ReporterGreen Fluorescent ProteinsHumansImmunity, CellularImmunoglobulinsLuminescent ProteinsMacaca fascicularisMembrane GlycoproteinsMonocytesSAIDS VaccinesSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransfectionTransplantation, AutologousConceptsMonocyte-derived dendritic cellsDendritic cellsAntigen-presenting cellsInterleukin-4Autologous monocyte-derived dendritic cellsSIV-specific immune responsesAutologous dendritic cellsDC marker CD83Macaque dendritic cellsGranulocyte-macrophage colonyMurine fibrocytesSIV vaccinesSIV antigensHumoral responseAntigen presentationImmune responseCharacteristic cellular morphologyDNA expression vectorsFibrocytesMacaca fascicularisSIVProtein expression plasmidsMacaquesExpression plasmidCells
1999
Detoxification of Methylglyoxal by the Nucleophilic Bidentate, Phenylacylthiazolium Bromide
Ferguson G, VanPatten S, Bucala R, Al-Abed Y. Detoxification of Methylglyoxal by the Nucleophilic Bidentate, Phenylacylthiazolium Bromide. Chemical Research In Toxicology 1999, 12: 617-622. PMID: 10409401, DOI: 10.1021/tx990007y.Peer-Reviewed Original ResearchConceptsEscherichia coli double mutantRapid cell deathE. coli cellsDetoxification of methylglyoxalExponential-phase cellsDouble mutantSensitive bacterial assayColi cellsCell deathPhase cellsE. coliLong-term complicationsPotassium channelsReactive dicarbonylsBacterial assaysDetoxificationEndogenous nucleophilesCellsMethylglyoxalTissue damage
1998
Role of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes
Sherry B, Zybarth G, Alfano M, Dubrovsky L, Mitchell R, Rich D, Ulrich P, Bucala R, Cerami A, Bukrinsky M. Role of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 1758-1763. PMID: 9465090, PMCID: PMC19180, DOI: 10.1073/pnas.95.4.1758.Peer-Reviewed Original ResearchConceptsHIV-1 infectionT lymphocytesPrimary peripheral blood mononuclear cellsPeripheral blood mononuclear cellsBlood mononuclear cellsHIV-1 virionsCyclophilin ACytokine-like mannerEarly eventsMononuclear cellsHIV therapyT cellsHIV-1Viral infectionViral infectivityInfectionCsA analoguesCellular receptorsLymphocytesCypAReceptorsCellsCell surfaceCD4Peptidyl-prolyl cis-trans isomerase activity
1997
Role of Macrophage Migration Inhibitory Factor in the Regulation of the Immune Response
Metz C, Bucala R. Role of Macrophage Migration Inhibitory Factor in the Regulation of the Immune Response. Advances In Immunology 1997, 66: 197-223. PMID: 9328642, DOI: 10.1016/s0065-2776(08)60598-2.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorT cellsImmune responseInhibitory factorAnti-inflammatory effectsInflammatory disease statesAnterior pituitary glandSeptic shockMIF actionMIF proteinInflammatory responseGlucocorticoid actionImmune systemPituitary glandCorticotrophic cellsGlucocorticoidsInhibitory effectDisease statesBaseline statePotential roleMacrophagesCellsResponseArthritis
1984
The reaction of 16α‐hydroxyestrone with erythrocytes in vitro and in vivo
BUCALA R, FISHMAN J, CERAMI A. The reaction of 16α‐hydroxyestrone with erythrocytes in vitro and in vivo. The FEBS Journal 1984, 140: 593-598. PMID: 6723653, DOI: 10.1111/j.1432-1033.1984.tb08143.x.Peer-Reviewed Original ResearchConceptsMembrane proteinsCellular proteinsCell lifeRed cell lifeLysine residuesProteinCovalent adductsHydrophobic chromatographyAcid-precipitable radioactivityRate of incorporationStable covalent adductCellsReverse-phase high-pressure liquid chromatographyElevated levelsResiduesAdduct formationRed cellsElectrophoresisVitro