2001
Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa
Eickhoff R, Wilhelm B, Renneberg H, Wennemuth G, Bacher M, Linder D, Bucala R, Seitz J, Meinhardt A. Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa. Molecular Medicine 2001, 7: 27-35. PMID: 11474125, PMCID: PMC1949991, DOI: 10.1007/bf03401836.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEpididymisImmunohistochemistryMacrophage Migration-Inhibitory FactorsMaleMicroscopy, ImmunoelectronRatsRNA, MessengerSpermatozoaConceptsOuter dense fibersSequence analysisDense fibersSecretion modeCDNA fragmentsN-terminal signal sequenceEndoplasmic reticulumMacrophage migration inhibitory factorEpithelial cellsCytokine macrophage migration inhibitory factorN-terminal amino acid sequence analysisAmino acid sequence analysisApical cell surfaceAcid sequence analysisEpididymal epithelial cellsRat epididymisSignal sequenceCDNA sequenceWestern blot experimentsPlasma membraneMigration inhibitory factorSecretory proteinsCytoskeletal elementsExpression patternsN-terminus
2000
Involvement of Macrophage Migration Inhibitory Factor (MIF) in Experimental Uric Acid Nephropathy
Kim Y, Huang X, Suga S, Mazzali M, Tang D, Metz C, Bucala R, Kivlighn S, Johnson R, Lan H. Involvement of Macrophage Migration Inhibitory Factor (MIF) in Experimental Uric Acid Nephropathy. Molecular Medicine 2000, 6: 837-848. PMID: 11126199, PMCID: PMC1949919, DOI: 10.1007/bf03401822.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsImmunohistochemistryKidney DiseasesKidney Function TestsMacrophage Migration-Inhibitory FactorsMaleRatsRats, Sprague-DawleyUp-RegulationUric AcidConceptsMacrophage migration inhibitory factorUric acid crystal depositionMigration inhibitory factorUric acid nephropathyGranuloma formationMIF mRNAMIF proteinOxonic acidInhibitory factorCrystal depositionAcute uric acid nephropathyAreas of granulomasMIF mRNA expressionProgressive tubulointerstitial injuryRoutine light microscopyUrate nephropathyWestern blot analysisMIF expressionLocal cellular responseMIF secretionTubulointerstitial damageTubulointerstitial injuryInterleukin-2RRenal diseaseDTH reactionMacrophage Migration Inhibitory Factor Release by Macrophages after Ingestion of Plasmodium chabaudi-Infected Erythrocytes: Possible Role in the Pathogenesis of Malarial Anemia
Martiney J, Sherry B, Metz C, Espinoza M, Ferrer A, Calandra T, Broxmeyer H, Bucala R. Macrophage Migration Inhibitory Factor Release by Macrophages after Ingestion of Plasmodium chabaudi-Infected Erythrocytes: Possible Role in the Pathogenesis of Malarial Anemia. Infection And Immunity 2000, 68: 2259-2267. PMID: 10722628, PMCID: PMC97412, DOI: 10.1128/iai.68.4.2259-2267.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnemiaAnimalsBone MarrowCells, CulturedDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayErythrocytesErythroid Precursor CellsErythropoiesisErythropoietinFemaleImmunohistochemistryLeukopoiesisLiverMacrophage Migration-Inhibitory FactorsMacrophagesMalariaMiceMice, Inbred BALB CMice, Inbred C3HPlasmodium chabaudiSpleenTime FactorsConceptsMacrophage migration inhibitory factorChabaudi-infected erythrocytesMalarial anemiaP. chabaudi-infected miceBALB/c miceP. chabaudi-infected erythrocytesTumor necrosis factor alphaMacrophage migration inhibitory factor releaseHuman falciparum malariaRed blood cell destructionPlasmodium falciparum infectionMigration inhibitory factorNecrosis factor alphaSuppression of erythropoiesisAntibody neutralization studiesBlood cell destructionHost-derived factorsPlasmodium chabaudi-infected erythrocytesErythropoiesis inhibitorMalaria anemiaActive diseaseCerebral malariaChabaudi infectionFalciparum malariaFalciparum infection
1999
Macrophage migration inhibitory factor in rheumatoid arthritis: Evidence of proinflammatory function and regulation by glucocorticoids
Leech M, Metz C, Hall P, Hutchinson P, Gianis K, Smith M, Weedon H, Holdsworth S, Bucala R, Morand E. Macrophage migration inhibitory factor in rheumatoid arthritis: Evidence of proinflammatory function and regulation by glucocorticoids. Arthritis & Rheumatism 1999, 42: 1601-1608. PMID: 10446857, DOI: 10.1002/1529-0131(199908)42:8<1601::aid-anr6>3.0.co;2-b.Peer-Reviewed Original ResearchMeSH KeywordsAgedArthritis, RheumatoidCells, CulturedCytokinesGlucocorticoidsHumansImmunohistochemistryInterferon-gammaLymphocyte ActivationMacrophage Migration-Inhibitory FactorsMacrophagesMonocytesReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSynovial MembraneT-LymphocytesTumor Necrosis Factor-alphaConceptsMacrophage migration inhibitory factorFibroblast-like synoviocytesRA fibroblast-like synoviocytesEnzyme-linked immunosorbent assayRheumatoid arthritisMigration inhibitory factorTNFalpha releaseExpression of MIFIntracellular macrophage migration inhibitory factorInhibitory factorCultured RA fibroblast-like synoviocytesCytokine macrophage migration inhibitory factorCultured fibroblast-like synoviocytesTumor necrosis factor-alpha synthesisHuman RA synoviumRA synovial tissueNecrosis factor-alpha synthesisHuman autoimmune diseasesPotential therapeutic targetTranscription-polymerase chain reactionMessenger RNA expressionT cell activationMIF expressionRA patientsRA synoviumAn Essential Role for Macrophage Migration Inhibitory Factor (MIF) in Angiogenesis and the Growth of a Murine Lymphoma
Chesney J, Metz C, Bacher M, Peng T, Meinhardt A, Bucala R. An Essential Role for Macrophage Migration Inhibitory Factor (MIF) in Angiogenesis and the Growth of a Murine Lymphoma. Molecular Medicine 1999, 5: 181-191. PMID: 10404515, PMCID: PMC2230298, DOI: 10.1007/bf03402061.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesCell DivisionCollagenDisease Models, AnimalDrug CombinationsEndothelium, VascularHumansImmunohistochemistryLamininLymphoma, B-CellMacrophage Migration-Inhibitory FactorsMiceMice, Inbred StrainsNeovascularization, PathologicOligonucleotides, AntisenseProteoglycansTumor Cells, CulturedConceptsMacrophage migration inhibitory factorRole of MIFAnti-MIF monoclonal antibodyMigration inhibitory factorB-cell lymphomaMonoclonal antibodiesCell lymphomaEffect of MIFBackgroundMacrophage migration inhibitory factorInhibitory factorC3H/HeN miceTumor-associated neovasculatureActivation of macrophagesAutocrine growth factorMicrovascular endothelial cellsCultured microvascular endothelial cellsAnti-neoplastic agentsNew blood vessel formationSolid tumor biologyEndothelial cell proliferationMIF expressionHeN miceSyngeneic modelMIF proteinTumor response
1998
Local Regulation of Macrophage Subsets in the Adult Rat Testis: Examination of the Roles of the Seminiferous Tubules, Testosterone, and Macrophage-Migration Inhibitory Factor1
Meinhardt A, Bacher M, Metz C, Bucala R, Wreford N, Lan H, Atkins R, Hedger M. Local Regulation of Macrophage Subsets in the Adult Rat Testis: Examination of the Roles of the Seminiferous Tubules, Testosterone, and Macrophage-Migration Inhibitory Factor1. Biology Of Reproduction 1998, 59: 371-378. PMID: 9687310, DOI: 10.1095/biolreprod59.2.371.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorSeminiferous tubule functionLeydig cell functionAdult rat testisMacrophage subsetsMacrophage numbersT implantsTubule functionAbdominal testesRat testisLeydig cellsResident macrophagesCytokine macrophage migration inhibitory factorMonocyte/macrophage subsetsCell functionMacrophage surface antigensResident macrophage numbersMacrophage migration inhibitoryMonoclonal antibody ED2MIF levelsLack of expressionSurface antigenInhibitory factorTestosterone implantsScrotal testesAdvanced Glycation Endproducts in Neurofilament Conglomeration of Motoneurons in Familial and Sporadic Amyotrophic Lateral Sclerosis
Chou S, Wang H, Taniguchi A, Bucala R. Advanced Glycation Endproducts in Neurofilament Conglomeration of Motoneurons in Familial and Sporadic Amyotrophic Lateral Sclerosis. Molecular Medicine 1998, 4: 324-332. PMID: 9642682, PMCID: PMC2230387, DOI: 10.1007/bf03401739.Peer-Reviewed Original ResearchMeSH KeywordsAmyotrophic Lateral SclerosisBrain ChemistryGlycation End Products, AdvancedHumansImmunohistochemistryMotor NeuronsNeurofilament ProteinsSpinal CordConceptsAmyotrophic lateral sclerosisSporadic amyotrophic lateral sclerosisNeurofilament proteinNeuronal inclusionsLateral sclerosisAGE formationNitric oxide-mediated responsesAnti-AGE antibodyAdvanced glycation endproductsMethodsParaffin sectionsMotor neuronsConclusionsThese dataAdvanced glycationGlycation endproductsNeuronal toxicityNitric oxideSpecific antibodiesProtein nitrationConcomitant inductionSclerosisSuperoxide dismutasePatientsAntibodiesPotent oxidantFree radicalsInvolvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis
Leech M, Metz C, Santos L, Peng T, Holdsworth S, Bucala R, Morand E. Involvement of macrophage migration inhibitory factor in the evolution of rat adjuvant arthritis. Arthritis & Rheumatism 1998, 41: 910-917. PMID: 9588744, DOI: 10.1002/1529-0131(199805)41:5<910::aid-art19>3.0.co;2-e.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalArthritis, ExperimentalBlotting, WesternCell CountCells, CulturedDose-Response Relationship, DrugEdemaEnzyme-Linked Immunosorbent AssayImmunohistochemistryMacrophage Migration-Inhibitory FactorsMacrophagesMaleNeutralization TestsRatsRats, Sprague-DawleySynovial MembraneConceptsMacrophage migration inhibitory factorRat adjuvant arthritisAdjuvant arthritisMigration inhibitory factorSynovial macrophagesRat synoviumLevels of MIFRole of MIFInhibitory factorMonoclonal antibodiesArthritis clinical scoresT cell accumulationDose-dependent inhibitionEnzyme-linked immunosorbentMIF treatmentAdjuvant injectionMAb treatmentClinical scoresED-1Leukocyte numbersSpecific monoclonal antibodiesClinical aspectsT cellsArthritisMacrophage activation
1997
Elevated AGE-Modified ApoB in Sera of Euglycemic, Normolipidemic Patients with Atherosclerosis: Relationship to Tissue AGEs
Stitt A, He C, Friedman S, Scher L, Rossi P, Ong L, Founds H, Li Y, Bucala R, Vlassara H. Elevated AGE-Modified ApoB in Sera of Euglycemic, Normolipidemic Patients with Atherosclerosis: Relationship to Tissue AGEs. Molecular Medicine 1997, 3: 617-627. PMID: 9323713, PMCID: PMC2230092, DOI: 10.1007/bf03401819.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAgingApolipoproteins BArteriosclerosisCarotid ArteriesCollagenEndothelium, VascularEnzyme-Linked Immunosorbent AssayFemaleGlycation End Products, AdvancedHumansImmunohistochemistryMacrophagesMaleMicroscopy, FluorescenceMiddle AgedReceptor for Advanced Glycation End ProductsReceptors, ImmunologicRegression AnalysisConceptsSmooth muscle cellsAGE-specific receptorsMononuclear cellsAtherosclerotic vascular diseaseOcclusive atherosclerotic diseaseDevelopment of hyperlipidemiaLipid-laden macrophagesYoung healthy personsEarly-stage lesionsCardiac bypass patientsAGE-R1Nondiabetic patientsAsymptomatic patientsAsymptomatic personsBypass patientsNormolipidemic patientsAtherosclerotic diseaseDistribution of ageVascular diseaseInflammatory responseLate-stage plaquesAtheromatous lesionsEarly lesionsFatty streaksNondiabetic etiologyInsulin secretion is regulated by the glucose-dependent production of islet β cell macrophage migration inhibitory factor
Waeber G, Calandra T, Roduit R, Haefliger J, Bonny C, Thompson N, Thorens B, Temler E, Meinhardt A, Bacher M, Metz C, Nicod P, Bucala R. Insulin secretion is regulated by the glucose-dependent production of islet β cell macrophage migration inhibitory factor. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 4782-4787. PMID: 9114069, PMCID: PMC20802, DOI: 10.1073/pnas.94.9.4782.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorInsulin releaseInsulin secretionBeta cellsRecombinant macrophage migration inhibitory factorInhibitory factorGlucose-induced insulin releasePancreatic islet beta cellsInsulin-secreting beta cellsINS-1 cell lineIslet beta cellsIsolated rat isletsInsulin secretion responseIslets of LangerhansConcentration-dependent mannerIslet cell productsImmune cellsT lymphocytesPituitary hormonesPerifusion studiesAutocrine fashionRat isletsSecretion responseGlucocorticoid stimulationRegulatory role for macrophage migration inhibitory factor in acute respiratory distress syndrome
Donnelly S, Haslett C, Reid P, Grant I, Wallace W, Metz C, Bruce L, Bucala R. Regulatory role for macrophage migration inhibitory factor in acute respiratory distress syndrome. Nature Medicine 1997, 3: 320-323. PMID: 9055860, DOI: 10.1038/nm0397-320.Peer-Reviewed Original ResearchConceptsAcute respiratory distress syndromeMigration inhibitory factorRespiratory distress syndromeDistress syndromeSignificant pro-inflammatory effectsInhibitory factorMacrophage migration inhibitory factorPro-inflammatory cytokine secretionPulmonary inflammatory responseAnti-inflammatory actionAnti-inflammatory effectsPro-inflammatory effectsIL-8 secretionConcentration-related fashionNovel therapeutic approachesInflammatory diseasesCytokine secretionInflammatory responseTherapeutic approachesAlveolar airspaceAlveolar cellsPatientsGlucocorticoidsSyndromeSecretionTNF-α Up-regulates Renal MIF Expression in Rat Crescentic Glomerulonephritis
Lan H, Yang N, Metz C, Mu W, Song Q, Nikolic-Paterson D, Bacher M, Bucala R, Atkins R. TNF-α Up-regulates Renal MIF Expression in Rat Crescentic Glomerulonephritis. Molecular Medicine 1997, 3: 136-144. PMID: 9085256, PMCID: PMC2230061, DOI: 10.1007/bf03401805.Peer-Reviewed Original ResearchConceptsRenal MIF expressionMigration inhibitory factorMIF expressionCrescentic glomerulonephritisRat crescentic glomerulonephritisMIF productionDay 1BackgroundMacrophage migration inhibitory factorSoluble TNF-α receptorTumor necrosis factor αResident kidney cellsSerum MIF levelsPotent proinflammatory mediatorPathogenesis of endotoxemiaNecrosis factor αInterstitial macrophage infiltrationTNF-α receptorExperimental crescentic glomerulonephritisMIF levelsMIF secretionRenal injuryRenal damageRenal diseaseProinflammatory mediatorsMacrophage accumulation
1996
Glycation and microglial reaction in lesions of Alzheimer's disease
Dickson D, Sinicropi S, Yen S, Ko L, Mattiace L, Bucala R, Vlassara H. Glycation and microglial reaction in lesions of Alzheimer's disease. Neurobiology Of Aging 1996, 17: 733-743. PMID: 8892346, DOI: 10.1016/0197-4580(96)00116-9.Peer-Reviewed Original ResearchConceptsAdvanced glycation end productsAGE immunoreactivityNeurofibrillary tanglesExtracellular neurofibrillary tanglesAmyloid depositsMicroglial reactionActivation of microgliaIntracellular neurofibrillary tanglesAlzheimer's disease brainPattern of immunoreactivityGlycation end productsTriple immunostainingDisease brainSenile plaquesDiffuse amyloidAlzheimer's diseaseImmunoreactivityCryostat sectionsDouble labelingAntibodiesMicrogliaLesionsPolyclonal antibodiesDiseaseGlycation
1995
Immunohistochemical detection of advanced glycosylation end products within the vascular lesions and glomeruli in diabetic nephropathy
Nishino T, Horii Y, Shiiki H, Yamamoto H, Makita Z, Bucala R, Dohi K. Immunohistochemical detection of advanced glycosylation end products within the vascular lesions and glomeruli in diabetic nephropathy. Human Pathology 1995, 26: 308-313. PMID: 7890283, DOI: 10.1016/0046-8177(95)90063-2.Peer-Reviewed Original ResearchConceptsAdvanced glycosylation end productsGlycosylation end productsSevere diffuse lesionsAnti-AGE antibodyComplications of diabetesInner elastic layerRenal complicationsDiabetic nephropathyAGE antibodyDiffuse lesionsNondiabetic individualsVascular lesionsPathogenic roleAGE accumulationHyaline depositsImmunohistochemical stainingPositive stainingImmunohistochemical detectionAdvanced glycosylationVascular intimaNormal agingSpecific antibodiesTissue distributionEnd productsAntibodies
1994
Circulating Fibrocytes Define a New Leukocyte Subpopulation That Mediates Tissue Repair
Bucala R, Spiegel L, Chesney J, Hogan M, Cerami A. Circulating Fibrocytes Define a New Leukocyte Subpopulation That Mediates Tissue Repair. Molecular Medicine 1994, 1: 71-81. PMID: 8790603, PMCID: PMC2229929, DOI: 10.1007/bf03403533.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBone MarrowBone Marrow CellsCD4 AntigensCell AdhesionCells, CulturedCentrifugationChimeraCollagenConnective TissueCytoskeletonDNA-Binding ProteinsDose-Response Relationship, RadiationFemaleFibroblastsFlow CytometryFluorescent Antibody TechniqueHumansImmunohistochemistryLeukocytesMaleMiceMice, Inbred BALB CMicroscopy, ElectronMolecular Sequence DataNuclear ProteinsPhenotypeSex-Determining Region Y ProteinTime FactorsTranscription FactorsTransplantation, HeterologousVimentinWound HealingConceptsTissue injuryLeukocyte subpopulationsScar formationLong-term remodelingFibroblast-like propertiesNormal wound repairConnective tissue scarConnective tissue elementsCell typesFibrotic responseTissue scarWound chambersPathological fibrotic responsesHost responseInjuryConnective tissueFibrocytesWound repairFibroblast propertiesTissue repairTissue elementsDistinctive phenotypeSubpopulationsTissueNovel cell types