2000
Neurotoxicity of Advanced Glycation End-Products for Cultured Cortical Neurons
Takeuchi M, Bucala R, Suzuki T, Ohkubo T, Yamazaki M, Koike T, Kameda Y, Makita Z. Neurotoxicity of Advanced Glycation End-Products for Cultured Cortical Neurons. Journal Of Neuropathology & Experimental Neurology 2000, 59: 1094-1105. PMID: 11138929, DOI: 10.1093/jnen/59.12.1094.Peer-Reviewed Original ResearchConceptsCortical neuronsAge 2Alzheimer's diseaseEnd-stage renal diseaseNeuronal cellsAdvanced glycation end productsDM HD patientsGreater cytopathic effectsSpecific antibodiesAnti-AGE antibodyCultured cortical neuronsDose-dependent increaseGlycation end productsBlood of individualsCultured neuronal cellsDiabetes mellitusDiabetic patientsRenal diseaseNeurodegenerative processesNormal controlsAdvanced glycationCytopathic effectEpitope 1Cytotoxic effectsMTT assay
1985
Nonenzymatic modification of lens crystallins by prednisolone induces sulfhydryl oxidation and aggregate formation: In vitro and in vivo studies
Bucala R, Manabe S, Urban R, Cerami A. Nonenzymatic modification of lens crystallins by prednisolone induces sulfhydryl oxidation and aggregate formation: In vitro and in vivo studies. Experimental Eye Research 1985, 41: 353-363. PMID: 4065253, DOI: 10.1016/s0014-4835(85)80026-9.Peer-Reviewed Original ResearchConceptsSteroid-induced cataractPossible pharmacological strategiesSteroid therapyLens crystallinsPharmacological strategiesLens opacitiesNonenzymatic modificationGlucocorticoid prednisoloneToxic manifestationsTherapeutic levelsPrednisoloneCataractogenic effectVivo studiesGlucocorticoidsCataractLens proteinsSulfhydryl oxidationGel filtration chromatographyPrevious studiesAddition of dithiothreitol